Apolipoprotein A-IV/ApoA4 Antibody (CL0468) - BSA Free

Immunohistochemistry-Paraffin: Apolipoprotein A-IV/ApoA4 Antibody (CL0468) [NBP2-52932] - Analysis in human small intestine and pancreas tissues using NBP2-52932 antibody. Corresponding APOA4 RNA-seq data are presented for the same tissues.

Western Blot: Apolipoprotein A-IV/ApoA4 Antibody (CL0468) [NBP2-52932] - Lane 1: Marker [kDa], Lane 2: Human plasma

Immunohistochemistry-Paraffin: Apolipoprotein A-IV/ApoA4 Antibody (CL0468) [NBP2-52932] -Staining of human small intestine shows strong cytoplasmic positivity in glandular cells.

Immunohistochemistry-Paraffin: Apolipoprotein A-IV/ApoA4 Antibody (CL0468) [NBP2-52932] -Staining of human duodenum shows strong cytoplasmic positivity in glandular cells.

Immunohistochemistry-Paraffin: Apolipoprotein A-IV/ApoA4 Antibody (CL0468) [NBP2-52932] -Staining of human colon shows moderate positivity in plasma in blood vessels.

Immunohistochemistry-Paraffin: Apolipoprotein A-IV/ApoA4 Antibody (CL0468) [NBP2-52932] -Staining of human pancreas shows no positivity in exocrine glandular cells as expected.

• Reactivity: Hu
• Applications: WB, IHC
• Clone: CL0468
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free

Apolipoprotein A-IV/ApoA4 Antibody (CL0468) - BSA Free Summary

• Immunogen: This antibody was developed using a recombinant protein derived from P06727, with the exact immunogen sequence remaining proprietary.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: APOA4
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Western Blot 1 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended.
• Control Peptide:

  Apolipoprotein A-IV/ApoA4 Recombinant Protein Antigen (NBP2-52932PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Protein A purified

Alternate Names for Apolipoprotein A-IV/ApoA4 Antibody (CL0468) - BSA Free

• apo-AIV
• apoA-IV
• Apolipoprotein A4
• Apolipoprotein AIV
• Apolipoprotein A-IV
• MGC142154
• MGC142156

Background

Apoliprotein (apo) A-IV gene contains 3 exons separated by two introns. A sequence polymorphism has been identified in the 3'UTR of the third exon. The primary translation product is a 396-residue preprotein which after proteolytic processing is secreted its primary site of synthesis, the intestine, in association with chylomicron particles. Although its precise function is not known, apo A-IV is a potent activator of lecithin-cholesterol acyltransferase in vitro.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Review for Apolipoprotein A-IV/ApoA4 Antibody (NBP2-52932) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer IHC-P Human 01/05/2022

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: Colon tissue
• Species: Human
• Lot: CL0468

FAQs for Apolipoprotein A-IV/ApoA4 Antibody (NBP2-52932). (Showing 1 - 1 of 1 FAQ).

1. I would like to buy antibodies APOA4 (apolipoprotein AIV) for the cows (to western blot). I know that You don't have the antibodies for this species. Could You propose the best atibodies which I could test (use) in my experiment?
   • All eight of our APOA4 antibodies were raised against the human protein, and all of them have currently only been tested against human samples. I ran an alignment for you and found that there is a 77% sequence homology between human and bovine APOA4, so it is possible that the antibodies raised against human APOA4 may recognise the bovine protein. Should you wish to try any of our antibodies with your samples, you may be interested in our Innovator's Reward Program, which allows you to try our primary antibodies in an untested species or application without the financial risk of failure. To participate you simply complete an online review with an image, detailing the positive or negative results of your study, and in return you receive a discount voucher for 100% of the purchase price of the reviewed product.

Bioinformatics

• Gene Symbol: APOA4