Fibrinogen gamma chain Antibody (HL1395) - Azide and BSA Free

Western Blot: Fibrinogen gamma chain Antibody (HL1395) - Azide and BSA Free [NBP3-25467] - Human plasma (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Fibrinogen gamma antibody [HL1395] (NBP3-25467) diluted at 1:100000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunocytochemistry/Immunofluorescence: Fibrinogen gamma chain Antibody (HL1395) - Azide and BSA Free [NBP3-25467] - Fibrinogen gamma antibody [HL1395] detects Fibrinogen gamma protein at cytoplasm by immunofluorescent analysis. Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Fibrinogen gamma stained by Fibrinogen gamma antibody [HL1395] (NBP3-25467) diluted at 1:50. Blue: Fluoroshield with DAPI . Scale bar= 10um.

• Reactivity: Hu
• Applications: WB, ICC/IF
• Clone: HL1395
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

Fibrinogen gamma chain Antibody (HL1395) - Azide and BSA Free Summary

• Description: Novus Biologicals Rabbit Fibrinogen gamma chain Antibody (HL1395) - Azide and BSA Free (NBP3-25467) is a recombinant monoclonal antibody validated for use in WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Recombinant protein encompassing a sequence within the center region of human Fibrinogen gamma chain. The exact sequence is proprietary.
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: FGG
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence Assay dependent
  • Western Blot 1:500-1:100000
• Theoretical MW: 52 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Bovine (82%), Porcine (84%).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Protein A purified

Alternate Names for Fibrinogen gamma chain Antibody (HL1395) - Azide and BSA Free

• fibrinogen gamma chain
• fibrinogen, gamma polypeptide

Background

The protein encoded by the Fibrinogen gamma chain gene is the gamma component of fibrinogen, a blood-borne glycoprotein comprised of three pairs of nonidentical polypeptide chains. Following vascular injury, fibrinogen is cleaved by thrombin to form fibrin which is the most abundant component of blood clots. In addition, various cleavage products of fibrinogen and fibrin regulate cell adhesion and spreading, display vasoconstrictor and chemotactic activities, and are mitogens for several cell types. Mutations in this gene lead to several disorders, including dysfibrinogenemia, hypofibrinogenemia and thrombophilia. Alternative splicing results in two transcript variants encoding different isoforms.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: FGG