FGFR1 [p Tyr653] Antibody - BSA Free

Western Blot: FGFR1 [p Tyr653] Antibody [NBP3-05382] - Western blot analysis of extracts of 293T cells, using FGFR1 pAb (NBP3-05382) at 1:1000 dilution.293T cells were treated by EGF (25 ug/mL) at 37? for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 30s.

• Reactivity: Hu, Mu, Rt
• Applications: WB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

FGFR1 [p Tyr653] Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit FGFR1 [p Tyr653] Antibody - BSA Free (NBP3-05382) is a polyclonal antibody validated for use in WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: A synthetic phosphorylated peptide around Y653 of human FGFR1 (NP_075598.2). IDYYK
• Modification: p Tyr653
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: FGFR1
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for FGFR1 [p Tyr653] Antibody - BSA Free

• basic fibroblast growth factor receptor 1
• BFGFR
• bFGF-R-1
• CD331 antigen
• CD331
• CEK
• EC 2.7.10
• EC 2.7.10.1
• FGF R1
• FGFBR
• FGFR1
• FGFR-1
• fibroblast growth factor receptor 1
• FLGH3
• FLJ99988
• Flt-2
• FLT2H4
• Fms-like tyrosine kinase 2
• fms-related tyrosine kinase 2
• H2
• H5
• HBGFR
• heparin-binding growth factor receptor
• hydroxyaryl-protein kinase
• KAL 2
• KAL2
• N-SAM
• OGD
• Proto-oncogene c-Fgr
• soluble FGFR1 variant 1
• soluble FGFR1 variant 2

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for FGFR1 Antibody (NBP3-05382). (Showing 1 - 2 of 2 FAQ).

1. I would like to inquire whether you have samples of the following antibodies: NBP1-61338, NB600-1287, NBP1-19481, NBP1-19864; The datasheets for these antibodies show different molecular weights of the detected proteins so its no altogether clear to me which one detects the real protein. To sort this out, we would be using FGFR1-deficient cells and would very much appreciate if you could supply us with small aliquots of these antibodies.
   • FGFR1 has multiple isoforms and is subject to multiple PTM's. The molecular weight can vary greatly depending on experimental conditions and samples used. We fully guarantee all of our products for the listed applications and species. If you cannot get a product to work in an application or species stated on our datasheet, our technical service team will troubleshoot with you to get it to work. If a product still does not work after troubleshooting, you can receive a free of charge replacement product or a full refund. Due to our 100% guarantee, we do not offer free of charge samples of our products. If a smaller sample size is available, it will be listed on our product page.
2. I am working in Multiple sclerosis group and mainly working with mice model and cell culture study. I need FGFR1 antibody against Mice for FACS analysis. If it is available can I get the sample to check with my cells? And any Oligodendrocyte markers against mice for FACS too.
   • We do have an antibody that has been tested in FLOW, NB600-1287, however it has not been tested in mouse. If you would like to test this antibody in mouse samples we have an Innovators Reward Program where we reward you for trying our antibody in species and applications that have not been previously tested.

Bioinformatics

• Gene Symbol: FGFR1