FGFR1 Antibody (M17D10)

Images

 
Western Blot: FGF R1 Antibody (M17D10) [NB100-2080] - Total protein from MOLT-4 (lane 1) and Ntera-2 (lane 2) was separated on a 7.5% gel by SDS-PAGE. Protein was transferred to PVDF membrane, blocked and probed with 1 ...read more
Immunocytochemistry/ Immunofluorescence: FGF R1 Antibody (M17D10) [NB100-2080] - FGFR1 (M17D10) antibody was tested in NTERA2 cells at 1:100 with DyLight 488 (Green) using a 40X objective. Actin and nuclei were ...read more
Immunohistochemistry-Paraffin: FGF R1 Antibody (M17D10) [NB100-2080] - IHC analysis of formalin-fixed paraffin-embedded tissue section of human esophageal squamous cell carcinoma (SCC) using mouse monoclonal FGF R1 ...read more
Flow Cytometry: FGF R1 Antibody (M17D10) [NB100-2080] - Analysis of FGF R1 in MCF-7 cells (1x10^6 cells/mL) were stained with FGF R1 antibody (NB100-2080, red) at 1:1000. Detected with FITC conjugated goat anti-mouse ...read more
Immunohistochemistry-Paraffin: FGF R1 Antibody (M17D10) [NB100-2080] - Analysis of FFPE tissue section of human normal skin using mouse monoclonal FGF R1 antibody (clone M17D10) at 5 ug/mL. Cells of the epidermal layer ...read more
Immunohistochemistry-Paraffin: FGF R1 Antibody (M17D10) [NB100-2080] - Analysis of FFPE tissue section of human liver using mouse monoclonal FGF R1 antibody (clone M17D10) at 5 ug/mL. The hepatocytes depicted an ...read more
Flow Cytometry: FGF R1 Antibody (M17D10) [NB100-2080] - Analysis of FGF R1 in HEK293 cells (2x10^6 cells/ml) were stained with FGF R1 antibody (NB100-2080, red) at 1:1000 dilution. Detected with FITC conjugated goat ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-P, IP, CyTOF-ready
Clone
M17D10
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

FGFR1 Antibody (M17D10) Summary

Immunogen
Recombinant human ectodomain of FGFr1b expressed in E. coli from pro23 to his415; antigen contained NH2-terminal gly-ser-pro-gly-ile and COOH-terminal glu-phe sequences.
Epitope
Epitope is within the sequence pro179 to lys195 in Ig loop II.
Localization
Cell Membrane
Specificity
Reacts with both alpha and beta isoforms.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
FGFR1
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
  • Flow Cytometry 1:500 - 1:1000
  • Immunocytochemistry/Immunofluorescence 10 ug/mL
  • Immunohistochemistry 5 ug/mL
  • Immunohistochemistry-Paraffin 5 ug/mL
  • Immunoprecipitation 1:10 - 1:500
  • CyTOF-ready
Application Notes
This antibody is CyTOF ready.
Publications
Read Publication using
NB100-2080 in the following applications:

  • WB
    1 publication

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 8575296). Not yet tested in other species.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for FGFR1 Antibody (M17D10)

  • basic fibroblast growth factor receptor 1
  • BFGFR
  • bFGF-R-1
  • CD331 antigen
  • CD331
  • CEK
  • EC 2.7.10
  • EC 2.7.10.1
  • FGF R1
  • FGFBR
  • FGFR1
  • FGFR-1
  • fibroblast growth factor receptor 1
  • FLGH3
  • FLJ99988
  • Flt-2
  • FLT2H4
  • Fms-like tyrosine kinase 2
  • fms-related tyrosine kinase 2
  • H2
  • H5
  • HBGFR
  • heparin-binding growth factor receptor
  • hydroxyaryl-protein kinase
  • KAL 2
  • KAL2
  • N-SAM
  • OGD
  • Proto-oncogene c-Fgr
  • soluble FGFR1 variant 1
  • soluble FGFR1 variant 2

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for FGFR1 Antibody (NB100-2080)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: WB.


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WB
(1)
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(1)
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Product General Protocols

View specific protocols for FGFR1 Antibody (NB100-2080): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for FGFR1 Antibody (NB100-2080). (Showing 1 - 2 of 2 FAQ).

  1. I would like to inquire whether you have samples of the following antibodies: NBP1-61338, NB600-1287, NBP1-19481, NBP1-19864; The datasheets for these antibodies show different molecular weights of the detected proteins so its no altogether clear to me which one detects the real protein. To sort this out, we would be using FGFR1-deficient cells and would very much appreciate if you could supply us with small aliquots of these antibodies.
    • FGFR1 has multiple isoforms and is subject to multiple PTM's. The molecular weight can vary greatly depending on experimental conditions and samples used. We fully guarantee all of our products for the listed applications and species. If you cannot get a product to work in an application or species stated on our datasheet, our technical service team will troubleshoot with you to get it to work. If a product still does not work after troubleshooting, you can receive a free of charge replacement product or a full refund. Due to our 100% guarantee, we do not offer free of charge samples of our products. If a smaller sample size is available, it will be listed on our product page.
  2. I am working in Multiple sclerosis group and mainly working with mice model and cell culture study. I need FGFR1 antibody against Mice for FACS analysis. If it is available can I get the sample to check with my cells? And any Oligodendrocyte markers against mice for FACS too.
    • We do have an antibody that has been tested in FLOW, NB600-1287, however it has not been tested in mouse. If you would like to test this antibody in mouse samples we have an Innovators Reward Program where we reward you for trying our antibody in species and applications that have not been previously tested.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB100-2080AF405
Alexa Fluor 488 Labeled NB100-2080AF488
Alexa Fluor 647 Labeled NB100-2080AF647
Alexa Fluor 700 Labeled NB100-2080AF700
Allophycocyanin Labeled NB100-2080APC
Biotin Labeled NB100-2080B
DyLight 350 Labeled NB100-2080UV
DyLight 405 Labeled NB100-2080V
DyLight 488 Labeled NB100-2080G
DyLight 550 Labeled NB100-2080R
DyLight 650 Labeled NB100-2080C
DyLight 680 Labeled NB100-2080FR
DyLight 755 Labeled NB100-2080IR
HRP Labeled NB100-2080H
PE Labeled NB100-2080PE
PerCP Labeled NB100-2080PCP

Additional Array Products

Bioinformatics Tool for FGFR1 Antibody (NB100-2080)

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PTMs for FGFR1 Antibody (NB100-2080)

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Research Areas for FGFR1 Antibody (NB100-2080)

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Blogs on FGFR1.

FGFR1 - regulating cell growth and proliferation in development and disease
The vertebrate fibroblast growth factor receptor (FGFR) family is an important group of proteins involved in embryonic development and the growth and proliferation of adult cells. Mutations in FGFR proteins can lead to pathologies including bone o...  Read full blog post.

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Bioinformatics

Gene Symbol FGFR1
Entrez
Uniprot