Human FGF basic/FGF2 Protein

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SDS-Page: Human FGF basic/FGF2 Protein [NBP2-34921]

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Func, PAGE
Concentration
LYOPH

Order Details

Human FGF basic/FGF2 Protein Summary

Description
A biologically active protein to FGF2.

Source: E. coli

Amino Acid Sequence: MPALPEDGGS GAFPPGHFKD PKRLYCKNGG FFLRIHPDGR VDGVREKSDPHIKLQLQAEE RGVVSIKGVC ANRYLAMKED GRLLASKCVT DECFFFERLESNNYNTYRSR KYTSWYVALK RTGQYKLGSK TGPGQKAILF LPMSAKS
Preparation
Method
Novus' biologically active proteins are stringently purified to provide only the safest and most highly effective proteins available. This protein was expressed in E. coli, purified by HPLC, QC tested by SDS-PAGE and Western Blot and validated on appropriate cell lines for bioactivity. All HPLC and bioactivity data is provided for your assurance.
Details of Functionality
FGF basic/FGF2 protein is fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine balb/c 3T3 cells is less than 0.05 ng/ml, corresponding to a specific activity of >2.0 x 10^7 IU/mg
Source
E. coli
Protein/Peptide Type
Biologically Active Protein
Gene
FGF2
Purity
>95% pure by SDS-PAGE
Endotoxin Note
Less than 1 EU/ug of endotoxin as determined by LAL method.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Concentration
LYOPH
Purity
>95% pure by SDS-PAGE
Reconstitution Instructions
Reconstitute with sterilized distilled water or 0.1% BSA aqueous buffer to a final concentration of 0.1 - 1.0 mg/ml.

Applications/Dilutions

Theoretical MW
16.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 2 Reviews rated 5
using
NBP2-34921 in the following application:

Publications
Read Publication using NBP2-34921.

Notes

This lyophilized preparation is stable at 2-8 degrees C, but should be kept at -20 degrees C for long term storage, preferably desiccated. Upon reconstitution, the preparation is most stable at -20 to -80 degrees C, and can be stored for one week at 2-8 degrees C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 degrees C to -80 degrees C. Avoid repeated freeze/thaw cycles.

Alternate Names for Human FGF basic/FGF2 Protein

  • basic fibroblast growth factor bFGF
  • Basic fibroblast growth factor
  • bFGF
  • FGF basic
  • FGF2
  • FGF-2
  • FGF2AS
  • FGFBprostatropin
  • fibroblast growth factor 2 (basic)
  • GFG1
  • HBGF-2
  • HBGH-2
  • heparin-binding growth factor 2
  • NUDT6
  • Prostatropin

Background

Human FGF-2 (bFGF), encoded by the FGF2 gene, is a member of the fibroblast growth factor (FGF) family. Fibroblast growth factor was found in pituitary extracts in 1973 and then tested in a bioassay that caused fibroblasts to proliferate. After further fractionating the extract using acidic and basic pH, two different forms have isolated that named "acidic fibroblast growth factor" (FGF-1) and "basic fibroblast growth factor" (FGF-2). Human FGF-2 (bFGF) shares 54 % amino acid sequence identity with aFGF. Affinity between FGF-2 (bFGF) and its receptors can be increased by heparin or heparan sulfate proteoglycan. FGF-2 (bFGF) plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. FGF-2 (bFGF) are also involved in a variety of biological processes, including embryonic development, morphogenesis, tissue repair, tumor growth and invasion. Additionally, FGF-2 (bFGF) is frequently used for a critical component of cell culture medium, e.g., human embryonic stem cell culture medium, serum-free culture systems.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 2 years from date of receipt.

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Publications for FGF basic/FGF2 Biologically Active Protein (NBP2-34921)(1)

Reviews for FGF basic/FGF2 Biologically Active Protein (NBP2-34921) (2) 52

Average Rating: 5
(Based on 2 reviews)

Reviews using NBP2-34921:
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Images Ratings Applications Species Date Details
 FGF basic/FGF2 NBP2-34921
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reviewed by:
Anonymous
01/04/2017
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Summary

CommentsI ranked this protein with 5 stars because the cell differentiation was successfully performed.

Comments

CommentsSDS PAGE gel with Coomassie blue staining showing detection of Human FGF basic/FGF 2 Protein ( NBP2-34921) (0.5 ug) at about 17 kDa
 FGF basic/FGF2 NBP2-34921
Enlarge
reviewed by:
Anonymous
01/04/2017
View

Summary

CommentsI ranked this protein with 5 stars because the cell differentiation was successfully performed.

Comments

CommentsSDS PAGE gel with Coomassie blue staining showing detection of 0.5 ug Human FGF basic protein (NBP2-34921-10 ug)

FAQs for FGF basic/FGF2 Biologically Active Protein (NBP2-34921). (Showing 1 - 1 of 1 FAQ).

  1. I am looking for an ovine antibody for bFGF and PDGF-AB and ELISA Kits for both. It is for a research work on meniscus healing in sheep. Do you have anything suitable? How is the homology with sheep? Is it ever tested and/or published in papers?
    • Unfortunately, most of the products you are interested in have not yet been tested in sheep, but this should not be a problem, due to sequence homology, and our Innovators Reward Program. In terms of an antibody for bFGF, also called FGF-2, we have two antibodies that are validated to detect the bovine protein, which is almost identical to that from sheep (99% homology). Here is a link to those FGF-2 antibodies. We only have FGF2 ELISA kits that have been tested with human or mouse. Mouse FGF-2 has 94% homology with sheep; human has 98% homology for most of its sequence, but also has an additional stretch of amino acids that is not present in the sheep protein. I therefore think that the mouse kit would be most suitable, just in case the human kit targets the region of the human protein not present in mouse. We sell antibodies to PDGF-A or PDGF-B rather than PDGF-AB. We don't have any PDGF-A antibodies that have been tested in sheep but there are several options you may find of interest. There isn't a sheep PDGFA sequence in UniProt, so I can't comment on how similar it is to the PDGFA from other species. Nevertheless, our NBP1-52533 PDGFB antibody has been shown to work in sheep. We sell two PDGF-AB ELISA kits, but these are for human and rat. I'm not sure how well these would work with sheep. Note that if you test a product with a species (or application) that it has not yet been validated in, you are eligible for our Innovator's Reward: Novus would provide you a 50% refund on the purchased product as well as a 50% discount on a future product of equal or lesser value. You would email innovators@novusbio.com to apply for your award, and we

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Recent Reviews

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2
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1
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Anonymous
01/04/2017
Application:
Species:

Anonymous
01/04/2017
Application:
Species:

Bioinformatics

Gene Symbol FGF2
Entrez
Uniprot

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