FGF-21 Antibody [Unconjugated]

Western blot shows lysates of mouse spleen tissue and rat spleen tissue. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse FGF‑21 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3057) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for FGF‑21 at approximately 26 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Mu
• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

FGF-21 Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant mouse FGF-21
  Tyr30-Ser210
  Accession # Q9JJN1
• Specificity: Detects mouse FGF-21 in direct ELISAs and Western blots. In these formats, approximately 15% cross‑reactivity with recombinant human (rh) FGF-21 is observed and less than 1% cross-reactivity with rhFGF-19 and rhFGF-23 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: FGF21
• Purity Statement: Antigen Affinity-purified
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Western Blot 0.25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for FGF-21 Antibody [Unconjugated]

• FGF21
• FGF-21
• fibroblast growth factor 21

Background

Mouse FGF-21 is a 20 kDa, secreted, nonglycosylated polypeptide that is a member of the FGF family. It is synthesized as a 210 amino acid (aa) precursor that contains a 28 aa signal sequence and a 182 aa mature region. Mature mouse FGF-21 shares 81%, 80%, 79% and 92% aa sequence identity with human, bovine, canine and rat FGF-21, respectively. FGF-21 seems to be produced by the liver and exhibits unique antidiabetogenic effects in fat.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for FGF-21 Antibody (AF3057)(6)

Publications using AF3057

• SM Turpin-Nol, P Hammerschm, W Chen, A Jais, K Timper, M Awazawa, S Brodesser, JC Brüning CerS1-Derived C18:0 Ceramide in Skeletal Muscle Promotes Obesity-Induced Insulin Resistance Cell Rep, 2019;26(1):1-10.e7. 2019 [PMID: 30605666] (Neutralization, Mouse)
• Chronic Over-expression of Fibroblast Growth Factor 21 Increases Bile Acid Biosynthesis by Opposing FGF15/19 Action EBioMedicine, 2016;0(0):. 2016 [PMID: 28041926] (ELISA Development (Capture), Mouse)
• Ost M, Werner F, Dokas J, Klaus S, Voigt A Activation of AMPKalpha2 is not crucial for mitochondrial uncoupling-induced metabolic effects but required to maintain skeletal muscle integrity. PLoS ONE, 2014;9(4):e94689. 2014 [PMID: 24732703] (Western Blot, Mouse)
• Johnson , Charis L, Mehmood , Rashid, Laing , Scott W, Stepniak , Camilla, Kharitonenkov , Alexei, Pin , Christop Silencing of the Fibroblast growth factor 21 gene is an underlying cause of acinar cell injury in mice lacking MIST1. Am J Physiol Endocrinol Metab, 2014;306(8):E916-28. 2014 [PMID: 24549397] (Western Blot, Human)
• Cyphert HA, Ge X, Kohan AB, Salati LM, Zhang Y, Hillgartner FB Activation of the farnesoid x receptor induces hepatic expression and secretion of fibroblast growth factor 21. J. Biol. Chem., 2012;287(30):25123-38. 2012 [PMID: 22661717] (Western Blot, Rat)
• Ogawa Y, Kurosu H, Yamamoto M, Nandi A, Rosenblatt KP, Goetz R, Eliseenkova AV, Mohammadi M, Kuro-o M BetaKlotho is required for metabolic activity of fibroblast growth factor 21. Proc. Natl. Acad. Sci. U.S.A., 2007;104(18):7432-7. 2007 [PMID: 17452648] (Western Blot, Human)

Bioinformatics

• Gene Symbol: FGF21
• Uniprot:
  • Q9JJN1()