FGF-21 Antibody [Unconjugated]

Images

 
Western blot shows lysates of mouse spleen tissue and rat spleen tissue. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse FGF‑21 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3057) ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

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FGF-21 Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant mouse FGF-21
Tyr30-Ser210
Accession # Q9JJN1
Specificity
Detects mouse FGF-21 in direct ELISAs and Western blots. In these formats, approximately 15% cross‑reactivity with recombinant human (rh) FGF-21 is observed and less than 1% cross-reactivity with rhFGF-19 and rhFGF-23 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
FGF21
Purity Statement
Antigen Affinity-purified
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.25 ug/mL
Publications
Read Publications using
AF3057 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for FGF-21 Antibody [Unconjugated]

  • FGF21
  • FGF-21
  • fibroblast growth factor 21

Background

Mouse FGF-21 is a 20 kDa, secreted, nonglycosylated polypeptide that is a member of the FGF family. It is synthesized as a 210 amino acid (aa) precursor that contains a 28 aa signal sequence and a 182 aa mature region. Mature mouse FGF-21 shares 81%, 80%, 79% and 92% aa sequence identity with human, bovine, canine and rat FGF-21, respectively. FGF-21 seems to be produced by the liver and exhibits unique antidiabetogenic effects in fat.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for FGF-21 Antibody (AF3057)(6)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 3 applications: ELISA Development (Capture), Neutralization, Western Blot.


Filter By Application
ELISA Development (Capture)
(1)
Neutralization
(1)
Western Blot
(4)
All Applications
Filter By Species
Human
(2)
Mouse
(3)
Rat
(1)
All Species
Showing Publications 1 - 6 of 6.
Publications using AF3057 Applications Species
SM Turpin-Nol, P Hammerschm, W Chen, A Jais, K Timper, M Awazawa, S Brodesser, JC Brüning CerS1-Derived C18:0 Ceramide in Skeletal Muscle Promotes Obesity-Induced Insulin Resistance Cell Rep, 2019;26(1):1-10.e7. 2019 [PMID: 30605666] (Neutralization, Mouse) Neutralization Mouse
Chronic Over-expression of Fibroblast Growth Factor 21 Increases Bile Acid Biosynthesis by Opposing FGF15/19 Action EBioMedicine, 2016;0(0):. 2016 [PMID: 28041926] (ELISA Development (Capture), Mouse) ELISA Development (Capture) Mouse
Ost M, Werner F, Dokas J, Klaus S, Voigt A Activation of AMPKalpha2 is not crucial for mitochondrial uncoupling-induced metabolic effects but required to maintain skeletal muscle integrity. PLoS ONE, 2014;9(4):e94689. 2014 [PMID: 24732703] (Western Blot, Mouse) Western Blot Mouse
Johnson , Charis L, Mehmood , Rashid, Laing , Scott W, Stepniak , Camilla, Kharitonenkov , Alexei, Pin , Christop Silencing of the Fibroblast growth factor 21 gene is an underlying cause of acinar cell injury in mice lacking MIST1. Am J Physiol Endocrinol Metab, 2014;306(8):E916-28. 2014 [PMID: 24549397] (Western Blot, Human) Western Blot Human
Cyphert HA, Ge X, Kohan AB, Salati LM, Zhang Y, Hillgartner FB Activation of the farnesoid x receptor induces hepatic expression and secretion of fibroblast growth factor 21. J. Biol. Chem., 2012;287(30):25123-38. 2012 [PMID: 22661717] (Western Blot, Rat) Western Blot Rat
Ogawa Y, Kurosu H, Yamamoto M, Nandi A, Rosenblatt KP, Goetz R, Eliseenkova AV, Mohammadi M, Kuro-o M BetaKlotho is required for metabolic activity of fibroblast growth factor 21. Proc. Natl. Acad. Sci. U.S.A., 2007;104(18):7432-7. 2007 [PMID: 17452648] (Western Blot, Human) Western Blot Human

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Bioinformatics

Gene Symbol FGF21
Uniprot