FGF-21 Antibody (461804) [Alexa Fluor® 750]

Images

 
There are currently no images for FGF-21 Antibody (IC25372S).

Every product we sell is backed by Novus' 100% Guarantee. If you have used this product, please submit your images and reviews to earn reward points.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow
Clone
461804
Clonality
Monoclonal
Host
Mouse
Conjugate
Alexa Fluor 750
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.

FGF-21 Antibody (461804) [Alexa Fluor® 750] Summary

Immunogen
E. coli-derived recombinant human FGF-21
His29-Ser209
Accession # Q9NSA1
Specificity
Detects human FGF-21 in direct ELISAs. In direct ELISAs, less than 10% cross-reactivity with recombinant mouse FGF-21, recombinant human (rh) FGF-3, -4, -6 or -10 is observed and no cross-reactivity with rhFGF-5 or rhFGF-9 is observed.
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
FGF21
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Intracellular Staining by Flow Cytometry 0.25-1 ug/10^6 cells
Application Notes
Flow Cytometry: Please use 0.25-1 ug of conjugated antibody per 10e6 cells.

Packaging, Storage & Formulations

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Buffer
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Preservative
0.09% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for FGF-21 Antibody (461804) [Alexa Fluor® 750]

  • FGF21
  • FGF-21
  • fibroblast growth factor 21

Background

Fibroblast growth factor 21 (FGF-21) is a member of the FGF gene family, which currently contains 22 human members. Based on its structure, it is further classified as an FGF-19 subfamily member. This subfamily includes FGF-19, -21, and -23. Like all other FGF subfamilies, FGF-19 subfamily members contain a 120 amino acid (aa) core FGF domain that exhibits a beta -trefoil structure (1, 2). Unlike other FGF subfamilies, FGF-19 subfamily members apparently exhibit poor binding to ECM, resulting in highly diffusible molecules (3). The cDNA for FGF-21 predicts a 209 aa polypeptide that contains a 28 aa signal sequence and a 181 aa mature region (4). Notably, FGF-21, as well as FGF-19 show limited binding to heparin (4). One potential alternate splice form has been reported. It shows a 43 aa substitution for the C‑terminal 12 aa of the standard form (5). Mature human FGF-21 shows 81% aa identity to mouse FGF-21, and is known to be active on mouse cells (4, 6). The FGF‑19 subfamily is considered endocrine in nature. All three subfamily members impact some aspect of metabolism, all three are induced by a nuclear receptor heterodimer that includes RXR, and all three utilize Klotho family members for signal transduction (7, 8, 9). FGF-21 is produced by hepatocytes in response to free fatty acid (FFA) stimulation of a PPARa/RXR dimeric complex (3, 7, 10, 11). This situation occurs clinically during starvation, or following the ingestion of a high-fat/low-carbohydrate diet. Upon FGF-21 secretion, white adipose tissue is induced to release FFAs from triglyceride stores. Once FFAs reach hepatocytes, they are oxidized and reduced to acetyl-CoA. The acetyl-CoA is recombined into 4-carbon ketone bodies (acetoacetate and beta -hydroxybutyrate), released, and transported to peripheral tissues for TCA processing and energy generation (11, 12).

  1. Itoh, N. and D.M. Ornitz (2004) Trends Genet. 20:563.
  2. Mohammadi, M. et al. (2005) Cytokine Growth Factor Rev. 16:107.
  3. Huang, X. et al. (2006) Mol. Carcinog. 45:934.
  4. Nishimura, T. et al. (2000) Biochim. Biophys. Acta 1492:203.
  5. GenBank Accession #: EAW52401 (2006).
  6. Ford, A.M. et al. (2005) J. Clin. Invest. 115:1627.
  7. Moore, D. D. (2007) Science 316:1436.
  8. Ogawa, Y. et al. (2007) Proc. Natl. Acad. Sci. USA 104:7432.
  9. Kurosu, H. et. al. (2007) J. Biol. Chem. 282:26687.
  10. Lundasen, T. et al. (2007) Biochem. Biophys. Res. Commun. 360:437.
  11. Badman, M.K. et al. (2007) Cell Metab. 5:426.
  12. Inagaki, T. et al. (2007) Cell Metab. 5:415.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP2-34260
Species: Hu, Mu, Po, Bv, Rb
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
NB300-537
Species: Hu, Mu, Rt, Bv, Rb
Applications: WB, ChIP, Flow, GS, ICC/IF, IHC, IHC-P, IP
NBP2-22106
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P, IP
NB600-1287
Species: Hu, Mu, Rt, Rb
Applications: WB, Flow, IHC, IP, CyTOF-ready
2629-FG
Species: Mu
NBP2-00932
Species: Hu
Applications: WB, Flow, ICC/IF, IHC, IHC-P
AF2619
Species: Mu
Applications: WB
NBP1-32822
Species: Hu, Mu, Rt, Al, Av, Bv, Ce, Pl
Applications: WB, ChIP, ICC/IF, IHC, IHC-P, IP, KD
MAB1249
Species: Hu, Mu
Applications: CyTOF-ready, ICC, ICFlow
AF887
Species: Hu, Mu, Rt
Applications: WB, Simple Western, IHC, CyTOF-ready, ICFlow
NBP2-34294
Species: Hu, Mu, Rt
Applications: WB, Flow, IHC, IHC-P
NBP2-22203
Species: Hu, Mu, Rt, Pm
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P
NB110-39113
Species: Hu, Mu, Rt, Rb
Applications: WB, ChIP, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, In vitro, Flow-IC
AF2265
Species: Mu
Applications: WB, IHC
IC25372S
Species: Hu
Applications: Flow

Publications for FGF-21 Antibody (IC25372S) (0)

There are no publications for FGF-21 Antibody (IC25372S).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for FGF-21 Antibody (IC25372S) (0)

There are no reviews for FGF-21 Antibody (IC25372S). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

FAQs for FGF-21 Antibody (IC25372S) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 IC25372U
Alexa Fluor 405 IC25372V
Alexa Fluor 488 IC25372G
Alexa Fluor 594 IC25372T
Alexa Fluor 647 IC25372R
Alexa Fluor 700 IC25372N
Alexa Fluor 750 IC25372S

Additional FGF-21 Products

Bioinformatics Tool for FGF-21 Antibody (IC25372S)

Discover related pathways, diseases and genes to FGF-21 Antibody (IC25372S). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for FGF-21 Antibody (IC25372S)

Discover more about diseases related to FGF-21 Antibody (IC25372S).
 

Pathways for FGF-21 Antibody (IC25372S)

View related products by pathway.

PTMs for FGF-21 Antibody (IC25372S)

Learn more about PTMs related to FGF-21 Antibody (IC25372S).

Blogs on FGF-21

There are no specific blogs for FGF-21, but you can read our latest blog posts.
Coronavirus Brochure

Customers Who Bought This Also Bought

Contact Information

Learn the difference between western blot and simple western

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our FGF-21 Antibody (461804) [Alexa Fluor® 750] and receive a gift card or discount.

Bioinformatics

Gene Symbol FGF21
Uniprot