Recombinant Human FGF-13 Protein

SDS-Page: Human FGF-13 Protein [NBP2-35009]

• Reactivity: Hu
• Applications: WB, PAGE
• Format: Carrier-Free
• Concentration: LYOPH

Recombinant Human FGF-13 Protein Summary

• Description: A single non-glycosylated polypeptide chain containing 245 amino acids corresponding to FGF-13 Source: E. coli

  Uniprot ID: Q92913

  Amino Acid Sequence: MAAAIASSLI RQKRQARERE KSNACKCVSS PSKGKTSCDK NKLNVFSRVK LFGSKKRRRR RPEPQLKGIV TKLYSRQGYH LQLQADGTID GTKDEDSTYT LFNLIPVGLR VVAIQGVQTK LYLAMNSEGY LYTSELFTPE CKFKESVFEN YYVTYSSMIY RQQQSGRGWY LGLNKEGEIM KGNHVKKNKP AAHFLPKPLK VAMYKEPSLH DLTEFSRSGS GTPTKSRSVS GVLNGGKSMS HNEST

• Preparation Method: Novus' biologically active proteins are stringently purified to provide only the safest and most highly effective proteins available. This protein was expressed in E. coli, purified by HPLC, QC tested by SDS-PAGE and Western Blot and validated on appropriate cell lines for bioactivity. All HPLC and bioactivity data is provided for your assurance.
• Details of Functionality: Test in process.
• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: FGF13
• Purity: >95%, by SDS-PAGE and HPLC
• Endotoxin Note: Less than 1 EU/ug of FGF-13 as determined by LAL method.

Applications/Dilutions

• Dilutions:
  • SDS-Page
  • Western Blot
• Theoretical MW: 27.6 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered concentrated solution in 20 mM Tris, pH 8.5, 500 mM NaCl.
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: >95%, by SDS-PAGE and HPLC
• Reconstitution Instructions: Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at <-20C.

Notes

This lyophilized preparation is stable at 2-8 degrees C, but should be kept at -20 degrees C for long term storage, preferably desiccated. Upon reconstitution, the preparation is most stable at -20 to -80 degrees C, and can be stored for one week at 2-8 degrees C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 degrees C to -80 degrees C. Avoid repeated freeze/thaw cycles.

Alternate Names for Recombinant Human FGF-13 Protein

• FGF13
• FGF-13
• FGF2Fibroblast growth factor homologous factor 2
• FHF2FHF-2
• fibroblast growth factor 13

Background

Human FGF-13 encoded by the FGF13 gene, belongs to the FGF-11 subfamily which has four members FGF-11 to FGF-14. These four members were initially referred to as fibroblast growth factor homologous factors (FHFs) because they have high sequence identity with the other FGFs but did not activate FGF receptors (FGFRs) and were not generally considered members of the FGF family. FGF-13 plays a crucial role in neuron polarization and migration in the cerebral cortex. In murine FGF-13 RNA was detected in developing central nervous system in cells, and was also found throughout the peripheral nervous system.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

Publications for FGF-13 Recombinant Protein (NBP2-35009)(2)

Publications using NBP2-35009

• Cheng Yalin, Gao Bin, Wang Haitao et al. Evolution of beak morphology in the Ground Tit revealed by comparative transcriptomics. Front Zool 2017-01-01 [PMID: 29299037] (Func, Human)
• Yu L, Toriseva M, Tuomala M et al. Increased expression of fibroblast growth factor 13 (FGF13) in prostate cancer is associated with shortened time to biochemical recurrence after radical prostatectomy. Int. J. Cancer. 2016-02-17 [PMID: 26891277] (WB)

FAQs for FGF-13 Recombinant Protein (NBP2-35009). (Showing 1 - 1 of 1 FAQs).

1. Have you tested your FGF13 protein with any cells, is it functional?
   • Our protein NBP2-35009, is a recent addition to our catalog and is fully covered by our guarantee for biologcal activity. This protein was prepared in a proven system for production of biologically active proteins. Activity assays are currently in progress.

Bioinformatics

• Gene Symbol: FGF13
• Uniprot:
  • Q92913(Human)