Recombinant Human FGF-10 Animal-Free Protein

SDS-Page: Recombinant Human FGF-10 Animal-Free Protein [NBP2-76198] - 1 ug in each lane (-) non-reducing conditions and (+) reducing conditions in a 4-20% Tris-Glycine gel.

Bioactivity: Recombinant Human FGF-10 Animal-Free Protein [NBP2-76198] - FGF-10 induced proliferation of 4MBR5 cells.

• Reactivity: Hu
• Applications: PAGE, Bioactivity
• Format: Animal Free
• Concentration: LYOPH

Recombinant Human FGF-10 Animal-Free Protein Summary

• Description: An un-tagged bioactive recombinant protein corresponding to Human FGF-10
  
  

  Source: E. coli

  Amino Acid Sequence: MLGQDMVSPE ATNSSSSSFS SPSSAGRHVR SYNHLQGDVR WRKLFSFTKY FLKIEKNGKV SGTKKENCPY SILEITSVEI GVVAVKAINS NYYLAMNKKG KLYGSKEFNNDCKLKERIEE NGYNTYASFN WQHNGRQMYV ALNGKGAPRR GQKTRRKNTS AHFLPMVVHS

  Produced with no materials of animal or human origin using ANIMAL-FREE purification processes.

  Production and Purification

  • No animal-derived ingredients, No FBS
  • E. coli based production system
  • Animal-Free facility, fermentor, columns, filters, labware, lyophilization, and storage
  
  

  Quality Assurance

  • Endotoxin levels at <1 EUs/ug
  • Purity levels at 95% or greater
• Specificity: FGF-10 is a monomer, 19.3 kDa (170 aa)
• Preparation Method: Protein is derived from genetically modified E. coli.
• Details of Functionality: 4MBr-5 cell proliferation, >5.0 x 10^3 units/mg (ED50 acceptance criteria are <200 ng/mL).
• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: FGF10
• Purity: >95%, by SDS-PAGE
• Endotoxin Note: Acceptance criteria for endotoxin levels are <1 EUs/ug protein (determined by Kinetic LAL Method).

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • SDS-Page
• Theoretical MW: 19.3 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.
• Buffer: Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 10 mM sodium phosphate, pH 7.5
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: >95%, by SDS-PAGE
• Reconstitution Instructions: Sterile water at 0.1 mg/mL

Notes

Lot Specific Information:
Given endotoxin and ED50 values are acceptance criteria standards. For lot specific values, please contact technical support.

Detailed Reconstitution:
Centrifuge vial before opening. Suspend the product by gently pipetting the recommended solution down the sides of the vial. DO NOT VORTEX. Allow several minutes for complete reconstitution. For prolonged storage, dilute to working aliquots.

Expiration Date:
12 months from date of receipt when stored at -20C to-80C as supplied. 1 month when stored at 4C after reconstituting as directed. 3 months when stored at -20C to -80C after reconstituting as directed.

Alternate Names for Recombinant Human FGF-10 Animal-Free Protein

• FGF10
• FGF-10
• fibroblast growth factor 10
• Keratinocyte growth factor 2
• KGF2
• KGF-2
• produced by fibroblasts of urinary bladder lamina propria

Background

The FGF10 gene encodes a 207 amino acid long, 23 kDA fibroblast growth factor 10 protein that is active in embryonic development, cell proliferation, and cell differentiation as it is a member of the fibroblast growth factor family (FGF). Additionally, the FGF10 gene functions in wound healing and is necessary for normal branching morphogenesis. FGF10 participates in regulation of actin cytoskeleton, mitochondrial apoptosis, paxillin interactions, nuclear receptor activation by vitamin-A, downstream signal transduction, and FGFR2 and FGFR ligand binding and activation. It is known to interact with genes FGF1, FGFR2, POMC, FGF18, and FGFBP1. FGF10 is linked to ladd syndrome, intestinal atresia, ectodermal dysplasia, craniosynostosis, cleft lip/palate, clear cell acanthoma, and gilles de la tourette syndrome.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: FGF10