Recombinant Human FGF-10 Protein

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications PAGE, Bioactivity
Concentration
Lyoph

Order Details

Recombinant Human FGF-10 Protein Summary

Description
Recombinant bioactive protein containing 170 amino acids for Human FGF-10

Source:E. coli
Amino Acid Sequence:(O15520)

Preparation
Method
Determined to be >98% pure by SDS-PAGE
Details of Functionality
The ED50 as determined by the dose-dependent stimulation of thymidine uptake by BaF3 cells expressing FGF receptors is <1 ng/ml, corresponding to a specific activity of >1 x 106 units/mg.
Source
E. coli
Protein/Peptide Type
Recombinant Protein
Gene
FGF10
Purity
>95%, by SDS-PAGE
Endotoxin Note
<0.1 ng/ug

Applications/Dilutions

Application Notes
This protein may appear as a haze or film, which may not be visible at the bottom of the vial.
Theoretical MW
19.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20C.
Buffer
Sterile filtered and lyophilized with no additives.
Concentration
Lyoph
Purity
>95%, by SDS-PAGE
Reconstitution Instructions
Centrifuge the vial prior to opening. Add sterile PBS, 50 ug sterile BSA per ug of FGF-10 to the vial to prepare a working stock solution of no less than 100 g/mL of FGF-10.

Alternate Names for Recombinant Human FGF-10 Protein

  • FGF10
  • FGF-10
  • fibroblast growth factor 10
  • Keratinocyte growth factor 2
  • KGF-2
  • produced by fibroblasts of urinary bladder lamina propria

Background

Fibroblast Growth Factor-10 (also called KGF-2) is a heparin binding growth factor that stimulates the proliferation and activation of cells that express FGF receptors. FGF-10 is mostly related to FGF-7/KGF and is expressed during development and preferentially in adult lungs. The human FGF-10 is a 19.3 kDa protein containing 170 amino acid residues.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Bioinformatics

Gene Symbol FGF10
Entrez