Recombinant Human FGF-10 Protein

Product Discontinued

Recombinant Human FGF-10 Protein Summary

• Description: Recombinant bioactive protein containing 170 amino acids for Human FGF-10
  
  

  Source:E. coli
  Amino Acid Sequence:(O15520)

• Preparation Method: Determined to be >98% pure by SDS-PAGE
• Details of Functionality: The ED50 as determined by the dose-dependent stimulation of thymidine uptake by BaF3 cells expressing FGF receptors is <1 ng/ml, corresponding to a specific activity of >1 x 106 units/mg.
• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: FGF10
• Purity: >98%, by SDS-PAGE
• Endotoxin Note: <0.1 ng/ug

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • SDS-Page
• Application Notes: This protein may appear as a haze or film, which may not be visible at the bottom of the vial.
• Theoretical MW: 19.3 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C.
• Buffer: Sterile filtered and lyophilized with no additives.
• Concentration: Lyoph
• Purity: >98%, by SDS-PAGE
• Reconstitution Instructions: Centrifuge the vial prior to opening. Add sterile PBS, 50 ug sterile BSA per ug of FGF-10 to the vial to prepare a working stock solution of no less than 100 g/mL of FGF-10.

Alternate Names for Recombinant Human FGF-10 Protein

• FGF10
• FGF-10
• fibroblast growth factor 10
• Keratinocyte growth factor 2
• KGF2
• KGF-2
• produced by fibroblasts of urinary bladder lamina propria

Background

Fibroblast Growth Factor-10 (also called KGF-2) is a heparin binding growth factor that stimulates the proliferation and activation of cells that express FGF receptors. FGF-10 is mostly related to FGF-7/KGF and is expressed during development and preferentially in adult lungs. The human FGF-10 is a 19.3 kDa protein containing 170 amino acid residues.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: FGF10