Fetuin B Antibody [Unconjugated]

Western blot shows human serum, human placenta tissue lysate, mouse serum, and rat placenta tissue lysate. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Human/Mouse/Rat Fetuin B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1725) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF017). A specific band was detected for Fetuin B at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western lane view shows lysates of human placenta tissue and rat placenta tissue, loaded at 0.2 mg/mL. A specific band was detected for Fetuin B at approximately 64 kDa (as indicated) using 2.5 µg/mL of Goat Anti-Human/Mouse/Rat Fetuin B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1725) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Western blot shows WT and Fetuin B KO serum from mouse. Nitrocellulose membrane was probed with 0.2 µg/mL of Goat Anti-Human/Mouse/Rat Fetuin B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1725) followed by HRP-conjugated donkey anti-goat IgG Secondary Antibody. A specific band was detected for Fetuin B at approximately 60 kDa (as indicated), but is not detectable in knockout sera. The Ponceau stained transfer of the blot is shown. This experiment was conducted under reducing conditions. Image, protocol, and testing courtesy of YCharOS Inc. See ycharos.com for additional details.

• Reactivity: Hu, Mu, Rt
• Applications: WB, Simple Western, KO
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Fetuin B Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Fetuin B
  Met19-Pro382
  Accession # Q9UGM5
• Specificity: Detects human Fetuin B in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 5% cross-reactivity with recombinant human (rh) Fetuin A, rhCystatin A, rhCystatin B, rhCystatin C, rhCystatin D, rhCystatin E/M, rhCystatin S, rhCystatin SA, and rhCystatin SN is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: FETUB
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Knockout Validated
  • Simple Western 2.5 ug/mL
  • Western Blot 0.25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
  *Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Fetuin B Antibody [Unconjugated]

• FETUB
• Fetuin B
• fetuin beta;fetuin phosphoprotein
• fetuin-B
• Fetuin-like protein IRL685,16G2
• Gugu
• IRL685

Background

Fetuins are members of the cystatin superfamily of cysteine protease inhibitors (1‑3). Additional members of this superfamily are kininogen and histidine-rich glycoprotein. Fetuin A and B are two known members of the fetuin family. Hepatocytes are believed to be the principal cellular source, but other cell types also express it (4, 5). Fetuin A, also known as alpha 2-Heremans-Schmid glycoprotein, is an inhibitor of basic calcium phosphate precipitation and a negative acute-phase protein (6, 7). Normal circulating levels of Fetuin A in adults (300‑600 ug/mL) fall significantly (30‑50%) during injury and infection (7). Fetuin B is a newer member whose function is not fully characterized (1, 2). Fetuin A and B display similarities and differences in their characteristics. Fetuin B exhibits reduction of calcification, while both mRNA levels were down‑regulated during the acute phase in inflammation-induced rats (4). However, they share only 20% amino acid sequence identity (2). The amounts of Fetuin B in human serum, unlike Fetuin A, vary with gender and are higher in females than in males (4).

1. Oliver, E. et al. (1999) Genomics. 57:352.
2. Oliver, E. et al. (2000) Biochem. J. 350:589.
3. Kellemann, J. et al. 1989, J. Biol. Chem. 264:14121.
4. Denecke, B. et al. (2003) Biochem. J. 376:135.
5. Schäfer, C. et al. (2003) J. Clin. Invest. 112:357.
6. Dziegielewska, K. M. et al. (1996) Histochem. Cell Biol. 106:319.
7. Gangneux, C. et al. (2003) Nucleic Acids Res. 31:5957.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Fetuin B Antibody (AF1725)(1)

Publication using AF1725

• Xu C, Yamamoto T, Zhou T, Aldrich CE, Frank K, Cullen JM, Jilbert AR, Mason WS The liver of woodchucks chronically infected with the woodchuck hepatitis virus contains foci of virus core antigen-negative hepatocytes with both altered and normal morphology. Virology, 2006-10-31;359(2):283-94. 2006-10-31 [PMID: 17078989] (ICC, IHC-P, Western Blot, Human, Woodchuck)

Bioinformatics

• Gene Symbol: FETUB
• Uniprot:
  • Q9UGM5()