Fetuin A/AHSG Antibody [Biotin]

• Reactivity: Hu
• Applications: WB, ELISA(Det)
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Biotin
• Concentration: LYOPH

Fetuin A/AHSG Antibody [Biotin] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Fetuin A
• Specificity: Detects human Fetuin A in ELISAs and Western blots. In sandwich immunoassays, less than 0.1% cross‑reactivity is observed with rmFetuin A.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: AHSG
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Western Blot 0.1 ug/mL
• Application Notes: ELISA Capture: Human Fetuin A/AHSG Antibody (Catalog # MAB11841)
  ELISA Detection: Human Fetuin A/AHSG Biotinylated Antibody (Catalog # BAF1184)
  Standard: Recombinant Human Fetuin A/AHSG (Catalog # 1184-PI)
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Fetuin A/AHSG Antibody [Biotin]

• A2HS
• AHS
• AHSG
• alpha-2-HS-glycoprotein
• alpha-2-Z-globulin
• Ba-alpha-2-glycoprotein
• FETUAba-alpha-2-glycoprotein
• Fetuin A
• fetuin-A
• HSGA

Background

Human Fetuin A, also known as alpha ₂-Heremans-Schmid glycoprotein, is encoded by the AHSG gene. It is a major plasma protein and a member of the cystatin superfamily of protease inhibitors (1, 2). It is expressed by hepatocytes, the principal cell source, and by monocyte/macrophages (3). The major form of plasma Fetuin A corresponds to a disulfide bond-linked two chains derived from the single chain (4). Human Fetuin A has a number of functions. It is a negative acute-phase protein with normal circulating levels in adults (300 - 600 μg/mL), which fall significantly (30 - 50%) during injury and infection (5). It enhances entry of cationic inhibitors into macrophages (6). It inhibits both insulin receptor autophosphorylation and undesirable calcification (6, 7). The purified rhFetuin A corresponds to the single chain, which can be converted to the two-chain form by rhFurin (R&D Systems, Catalog # 1503-SE) in vitro. However, the conversion does not enhance its inhibitory activity against rhCathpesin V, a cysteine protease.

1. Kellemann, J. et al. (1989) J. Biol. Chem. 264:14121.
2. Dziegielewska, K.M. et al. (1990) J. Biol. Chem. 265:4354.
3. Dziegielewska, K.M. et al. (1996) Histochem. Cell Biol. 106:319.
4. Gejyo, F. and K. Schmid (1981) Biochim. Biophys. Acta. 671:78.
5. Wang, H. et al. (1998) Proc. Natl. Acad. Sci. USA 95:14429.
6. Mathews, S.T. et al. (2000) Mol. Cell Endocrinol. 164:87.
7. Schäfer, C. et al. (2003) J. Clin. Invest. 112:357.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Fetuin A/AHSG Antibody (BAF1184)(2)

Publications using BAF1184

• Sarah Jesse, Stefan Lehnert, Olaf Jahn, Lucilla Parnetti, Hilkka Soininen, Sanna-Kaisa Herukka et al. Differential Sialylation of Serpin A1 in the Early Diagnosis of Parkinson’s Disease Dementia PLoS ONE 11/8/2012 [PMID: 23144969]
• Raoult D, Drancourt M, Azza S, Nappez C, Guieu R, Rolain JM, Fourquet P, Campagna B, La Scola B, Mege JL, Mansuelle P, Lechevalier E, Berland Y, Gorvel JP, Renesto P Nanobacteria are mineralo fetuin complexes. PLoS Pathog., 2008-02-08;4(2):e41. 2008-02-08 [PMID: 18282102] (ELISA Development, Human)

Bioinformatics

• Gene Symbol: AHSG