Fetuin A/AHSG Antibody [Unconjugated]

• Reactivity: Hu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Fetuin A/AHSG Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Fetuin A/AHSG
• Specificity: Detects human Fetuin A in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant mouse Fetuin A and recombinant rat Fetuin A is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: AHSG
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.1 ug/mL
  • Immunohistochemistry 5-15 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Fetuin A/AHSG Antibody [Unconjugated]

• A2HS
• AHS
• AHSG
• alpha-2-HS-glycoprotein
• alpha-2-Z-globulin
• Ba-alpha-2-glycoprotein
• FETUAba-alpha-2-glycoprotein
• Fetuin A
• fetuin-A
• HSGA

Background

Human Fetuin A, also known as alpha ₂-Heremans-Schmid glycoprotein, is encoded by the AHSG gene. It is a major plasma protein and a member of the cystatin superfamily of protease inhibitors (1, 2). It is expressed by hepatocytes, the principal cell source, and by monocyte/macrophages (3). The major form of plasma Fetuin A corresponds to a disulfide bond-linked two chains derived from the single chain (4). Human Fetuin A has a number of functions. It is a negative acute-phase protein with normal circulating levels in adults (300-600 μg/mL), which fall significantly (30-50%) during injury and infection (5). It enhances entry of cationic inhibitors into macrophages (6). It inhibits both insulin receptor autophosphorylation and undesirable calcification (6, 7). The purified rhFetuin A corresponds to the single chain, which can be converted to the two-chain form by rhFurin (R&D Systems, Catalog # 1503-SE) in vitro. However, the conversion does not enhance its inhibitory activity against rhCathpesin V, a cysteine protease.

1. Kellemann, J. et al. (1989) J. Biol. Chem. 264:14121.
2. Dziegielewska, K.M. et al. (1990) J. Biol. Chem. 265:4354.
3. Dziegielewska, K.M. et al. (1996) Histochem. Cell Biol. 106:319.
4. Gejyo, F. and K. Schmid (1981) Biochim. Biophys. Acta. 671:78.
5. Wang, H. et al. (1998) Proc. Natl. Acad. Sci. USA 95:14429.
6. Mathews, S.T. et al. (2000) Mol. Cell Endocrinol. 164:87.
7. Schäfer, C. et al. (2003) J. Clin. Invest. 112:357.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Fetuin A/AHSG Antibody (AF1184)(3)

Publications using AF1184

• C Sujana, C Huth, A Zierer, S Meesters, J Sudduth-Kl, W Koenig, C Herder, A Peters, B Thorand Association of fetuin-A with incident type 2 diabetes: Results from the MONICA/KORA Augsburg study and a systematic meta-analysis Eur. J. Endocrinol., 2018;0(0):. 2018 [PMID: 29439057] (Single Molecule Capture - Dete, Human)
• Li, Qiaoli, Brodsky, Jill L, Conlin, Laura K, Pawel, Bruce, Glatz, Andrew C, Gafni, Rachel I, Schurgers, Leon, Uitto, Jouni, Hakonarson, Hakon, Deardorff, Matthew, Levine, Michael Mutations in the ABCC6 gene as a cause of generalized arterial calcification of infancy: genotypic overlap with pseudoxanthoma elasticum. J Invest Dermatol, 2014;134(3):658-65. 2014 [PMID: 24008425] (IHC-P, Human)
• Li Q, Grange DK, Armstrong NL, Whelan AJ, Hurley MY, Rishavy MA, Hallgren KW, Berkner KL, Schurgers LJ, Jiang Q, Uitto J Mutations in the GGCX and ABCC6 genes in a family with pseudoxanthoma elasticum-like phenotypes. J. Invest. Dermatol., 2009;129(3):553-63. 2009 [PMID: 18800149] (IHC-P, Human)

Bioinformatics

• Gene Symbol: AHSG