Fetuin A/AHSG Antibody [Unconjugated]

Western blot shows human plasma. PVDF membrane was probed with 0.1 µg/mL of Goat Anti-Human Fetuin A/AHSG Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1184) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Fetuin A/AHSG at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Fetuin A/AHSG was detected in immersion fixed paraffin-embedded sections of human liver array using Goat Anti-Human Fetuin A/AHSG Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1184) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

• Reactivity: Hu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Fetuin A/AHSG Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Fetuin A/AHSG
• Specificity: Detects human Fetuin A in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant mouse Fetuin A and recombinant rat Fetuin A is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: AHSG

Applications/Dilutions

• Dilutions:
  • Western Blot 0.1 ug/mL
  • Immunohistochemistry 5-15 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Fetuin A/AHSG Antibody [Unconjugated]

• A2HS
• AHS
• AHSG
• alpha-2-HS-glycoprotein
• alpha-2-Z-globulin
• Ba-alpha-2-glycoprotein
• FETUAba-alpha-2-glycoprotein
• Fetuin A
• fetuin-A
• HSGA

Background

Human Fetuin A, also known as alpha ₂-Heremans-Schmid glycoprotein, is encoded by the AHSG gene. It is a major plasma protein and a member of the cystatin superfamily of protease inhibitors (1, 2). It is expressed by hepatocytes, the principal cell source, and by monocyte/macrophages (3). The major form of plasma Fetuin A corresponds to a disulfide bond-linked two chains derived from the single chain (4). Human Fetuin A has a number of functions. It is a negative acute-phase protein with normal circulating levels in adults (300-600 μg/mL), which fall significantly (30-50%) during injury and infection (5). It enhances entry of cationic inhibitors into macrophages (6). It inhibits both insulin receptor autophosphorylation and undesirable calcification (6, 7). The purified rhFetuin A corresponds to the single chain, which can be converted to the two-chain form by rhFurin (R&D Systems, Catalog # 1503-SE) in vitro. However, the conversion does not enhance its inhibitory activity against rhCathpesin V, a cysteine protease.

1. Kellemann, J. et al. (1989) J. Biol. Chem. 264:14121.
2. Dziegielewska, K.M. et al. (1990) J. Biol. Chem. 265:4354.
3. Dziegielewska, K.M. et al. (1996) Histochem. Cell Biol. 106:319.
4. Gejyo, F. and K. Schmid (1981) Biochim. Biophys. Acta. 671:78.
5. Wang, H. et al. (1998) Proc. Natl. Acad. Sci. USA 95:14429.
6. Mathews, S.T. et al. (2000) Mol. Cell Endocrinol. 164:87.
7. Schäfer, C. et al. (2003) J. Clin. Invest. 112:357.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Fetuin A/AHSG Antibody (AF1184)(3)

Publications using AF1184

• C Sujana, C Huth, A Zierer, S Meesters, J Sudduth-Kl, W Koenig, C Herder, A Peters, B Thorand Association of fetuin-A with incident type 2 diabetes: Results from the MONICA/KORA Augsburg study and a systematic meta-analysis Eur. J. Endocrinol., 2018;0(0):. 2018 [PMID: 29439057] (Single Molecule Capture - Dete, Human)
• Li, Qiaoli, Brodsky, Jill L, Conlin, Laura K, Pawel, Bruce, Glatz, Andrew C, Gafni, Rachel I, Schurgers, Leon, Uitto, Jouni, Hakonarson, Hakon, Deardorff, Matthew, Levine, Michael Mutations in the ABCC6 gene as a cause of generalized arterial calcification of infancy: genotypic overlap with pseudoxanthoma elasticum. J Invest Dermatol, 2014;134(3):658-65. 2014 [PMID: 24008425] (IHC-P, Human)
• Li Q, Grange DK, Armstrong NL, Whelan AJ, Hurley MY, Rishavy MA, Hallgren KW, Berkner KL, Schurgers LJ, Jiang Q, Uitto J Mutations in the GGCX and ABCC6 genes in a family with pseudoxanthoma elasticum-like phenotypes. J. Invest. Dermatol., 2009;129(3):553-63. 2009 [PMID: 18800149] (IHC-P, Human)

Bioinformatics

• Gene Symbol: AHSG