Fetal Hemoglobin Antibody - BSA Free

• Reactivity: Hu
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

Fetal Hemoglobin Antibody - BSA Free Summary

• Immunogen: Purified human hemoglobin F of fetal cord blood cell origin.
• Specificity: By immunoelectrophoresis and ELISA this antibody reacts specifically with human hemoglobin F. No antibody was detected against hemoglobin A1, A2, S or human serum proteins.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: HBG1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:1000 - 1:30000
  • Western Blot 1:1000-1:30000
• Application Notes: ELISA for coating plates 1:100 - 1:500.
• Control:

  HBG1/2 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Fetal Hemoglobin Antibody - BSA Free

• abnormal hemoglobin
• A-gamma globin
• gamma A hemoglobin
• gamma globin
• gamma-1-globin
• gamma-2-globin
• gamma-globin chain
• G-gamma globin Paulinia
• Hb F Agamma
• hb F Ggamma
• HBGA
• HBGR
• HBG-T1
• HBG-T2
• Hemoglobin gamma-1 chain
• hemoglobin gamma-2 chain
• Hemoglobin gamma-A chain
• hemoglobin gamma-G chain
• hemoglobin subunit gamma-1
• hemoglobin subunit gamma-2
• hemoglobin, gamma A
• hemoglobin, gamma, regulator of
• HSGGL1
• methemoglobin
• PRO2979

Background

The gamma globin genes (HBG1 and HBG2) are normally expressed in the fetal liver, spleen and bone marrow. Two gamma chains together with two alpha chains constitute fetal hemoglobin (HbF) which is normally replaced by adult hemoglobin (HbA) at birth. In some beta-thalassemias and related conditions, gamma chain production continues into adulthood. The two types of gamma chains differ at residue 136 where glycine is found in the G-gamma product (HBG2) and alanine is found in the A-gamma product (HBG1). The former is predominant at birth. The order of the genes in the beta-globin cluster is: 5'-epsilon -- gamma-G -- gamma-A -- delta -- beta--3'.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Fetal Hemoglobin Antibody (NB110-41084)(7)

Publications using NB110-41084

• Huang P, Peslak SA, Ren R et al. HIC2 controls developmental hemoglobin switching by repressing BCL11A transcription Nature genetics 2022-09-01 [PMID: 35941187] (FLOW, Human, Mouse)
• Qin K, Huang P, Feng R et al. Dual function NFI factors control fetal hemoglobin silencing in adult erythroid cells Nature genetics 2022-05-26 [PMID: 35618846] (FLOW, Human)
• Huang P, Peslak SA, Lan X et al. HRI-regulated transcription factor ATF4 activates BCL11A transcription to silence fetal hemoglobin expression Blood 2020-04-16 [PMID: 32299090]
• Khandros E, Huang P, Peslak SA, Sharma M Understanding Heterogeneity of Fetal Hemoglobin Induction through Comparative Analysis of F-and A-erythroblasts Blood 2020-01-01 [PMID: 32268371]
• Makino T, Haruyama M, Katayama K et al. Phenotypic-screening generates active novel fetal globin-inducers that downregulate Bcl11a in a monkey model Biochem. Pharmacol. 2019-11-18 [PMID: 31751536] (ELISA, Human)
• Zhang X, Luo YM, Ma YN et al. In vitro maturation of erythroid progenitors from human umbilical cord blood and patterns of globin gene expression: serum from different developmental stage plays important roles in liquid culture. Biochem Biophys Res Commun 2005-10-01 [PMID: 16125140]
• Umeda K, Heike T, Yoshimoto M et al. Development of primitive and definitive hematopoiesis from nonhuman primate embryonic stem cells in vitro. Development 2004-04-01 [PMID: 15084470]

FAQs for Fetal Hemoglobin Antibody (NB110-41084). (Showing 1 - 2 of 2 FAQs).

1. May I know if NB110-41084 is specific only to the gamma chain of Hemoglobin F and does not exhibit cross-reactivity with the Hemoglobin A chain? There are several anti-hemoglobin F antibodies on the market that exhibit cross-reactivity with the A chain; we are looking for an antibody that does NOT exhibit residual activity with the A chain.
   • For NB110-41084 please see Summary section of the datasheet. Specificity: "Hemoglobin F. No antibody was detected against hemoglobin A1, A2, S or human serum proteins." It appears that from testing, this antibody does not cross-react with Hemoglobin A1 or A2.
2. Hello! I am looking at product NB110-41084, Fetal Hemoglobin antibody. Can you tell me if this cross reacts with Regular Hemoglobin typically found in adults?
   • No reactivity was detected against hemoglobin A1, A2, S or human serum proteins. So no, it will not crossreact with adult hemoglobin

Bioinformatics

• Gene Symbol: HBG1
• Entrez:
  • 3048(Human)
• Uniprot:
  • P69892(Human)