Fetal Hemoglobin Antibody [Alexa Fluor® 647]

Flow Cytometry: Sheep Fetal Hemoglobin pAb [Alexa Fluor® 647] [NB110-41084AF647] - HbF cells in mouse. These mice are under stressed erythropoiesis. This antibody used to detect fetal hemoglobin in RBC. RBC was fixed by paraformaldehyde followed by permeabilization with 0.01% triton. These cells were incubated with antibody at 1:500 dilution for 30-60 min at room temperature. Image from a verified customer review.

• Reactivity: Hu
• Applications: WB, ELISA, Flow
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Alexa Fluor 647

Fetal Hemoglobin Antibody [Alexa Fluor® 647] Summary

• Immunogen: Purified human hemoglobin F of fetal cord blood cell origin.
• Specificity: By immunoelectrophoresis and ELISA this antibody reacts specifically with human hemoglobin F. No antibody was detected against hemoglobin A1, A2, S or human serum proteins.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: HBG1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Flow Cytometry Validated for Flow from a verified customer review.
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Immunogen affinity purified

Notes

Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for Fetal Hemoglobin Antibody [Alexa Fluor® 647]

• abnormal hemoglobin
• A-gamma globin
• gamma A hemoglobin
• gamma globin
• gamma-1-globin
• gamma-2-globin
• gamma-globin chain
• G-gamma globin Paulinia
• Hb F Agamma
• hb F Ggamma
• HBGA
• HBGR
• HBG-T1
• HBG-T2
• Hemoglobin gamma-1 chain
• hemoglobin gamma-2 chain
• Hemoglobin gamma-A chain
• hemoglobin gamma-G chain
• hemoglobin subunit gamma-1
• hemoglobin subunit gamma-2
• hemoglobin, gamma A
• hemoglobin, gamma, regulator of
• HSGGL1
• methemoglobin
• PRO2979

Background

The gamma globin genes (HBG1 and HBG2) are normally expressed in the fetal liver, spleen and bone marrow. Two gamma chains together with two alpha chains constitute fetal hemoglobin (HbF) which is normally replaced by adult hemoglobin (HbA) at birth. In some beta-thalassemias and related conditions, gamma chain production continues into adulthood. The two types of gamma chains differ at residue 136 where glycine is found in the G-gamma product (HBG2) and alanine is found in the A-gamma product (HBG1). The former is predominant at birth. The order of the genes in the beta-globin cluster is: 5'- epsilon -- gamma-G -- gamma-A -- delta -- beta--3'.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Review for Fetal Hemoglobin Antibody (NB110-41084AF647) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Mouse.

reviewed by: Verified Customer Flow Mouse 05/12/2025

Summary

• Application: Flow Cytometry
• Sample Tested: Whole blood
• Species: Mouse
• Lot: D179848

Comments

• Comments: ***Bio-Techne Response: This review reflects a new species or application tested on a primary antibody.***
  These mice are under stressed erythropoiesis. This antibody used to detect fetal hemoglobin in RBC. RBC was fixed by paraformaldehyde followed by permeabilization with 0.01% triton. These cells were incubated with antibody at 1:500 dilution for 30-60 min at room temperature.

Bioinformatics

• Gene Symbol: HBG1