Feline IL-10 DuoSet ELISA, 15 Plate

• Reactivity: Fe
• Applications: ELISA
• Conjugate: Biotin

Feline IL-10 DuoSet ELISA, 15 Plate Summary

• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: IL10

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Feline IL-10 DuoSet ELISA, 15 Plate

• CSIF
• CSIFMGC126450
• Cytokine synthesis inhibitory factor
• GVHDS
• IL10
• IL-10
• IL10A
• IL-10MGC126451
• interleukin 10
• interleukin-10
• TGIF

Background

Interleukin-10 functions as an anti-inflammatory cytokine by inhibiting the expansion and activation of Th1 cells and Th17 cells and by promoting the development of M2 macrophages and regulatory T cells (Treg). Within a tumor microrenvironment, however, IL-10 can inhibit the expansion of both Treg and myeloid-derived suppressor cells (MDSC). IL-10 exerts protective effects including limiting tissue damage in arthritic inflammation and promoting muscle regeneration after injury, but it also contributes to the persistence of viral infections. IL-10 signals through a receptor complex composed of IL-10 R alpha and IL-10 R beta. IL-10 R beta additionally associates with IL-20 R alpha, IL-22 R alpha 1, or IL-28 R alpha to form the receptor complexes for IL-22, IL-26, IL-28, and IL-29.

Publications for IL-10 (DY736)(6)

Publications using DY736

• KC Clark, FA Fierro, EM Ko, NJ Walker, B Arzi, CG Tepper, H Dahlenburg, A Cicchetto, A Kol, L Marsh, WJ Murphy, N Fazel, DL Borjesson Human and feline adipose-derived mesenchymal stem cells have comparable phenotype, immunomodulatory functions, and transcriptome Stem Cell Res Ther, 2017-03-20;8(1):69. 2017-03-20 [PMID: 28320483] (Feline)
• Verbrugghe A, Janssens G, Van de Velde H, Cox E, De Smet S, Vlaeminck B, Hesta M Failure of a dietary model to affect markers of inflammation in domestic cats. BMC Vet Res, 2014-05-04;10(0):104. 2014-05-04 [PMID: 24885092] (Feline)
• Schlecht-Louf G, Mangeney M, El-Garch H, Lacombe V, Poulet H, Heidmann T A targeted mutation within the feline leukemia virus (FeLV) envelope protein immunosuppressive domain to improve a canarypox virus-vectored FeLV vaccine. J Virol, 2013-11-06;88(2):992-1001. 2013-11-06 [PMID: 24198407] (Feline)
• Wood BA, O'Halloran KP, Vandewoude S Development and validation of a multiplex microsphere-based assay for detection of domestic cat (Felis catus) cytokines. Clin. Vaccine Immunol., 2011-01-05;18(3):387-92. 2011-01-05 [PMID: 21209158] (Feline)
• Declue AE, Williams KJ, Sharp C, Haak C, Lechner E, Reinero CR Systemic response to low-dose endotoxin infusion in cats. Vet. Immunol. Immunopathol., 2009-06-11;0(0):. 2009-06-11 [PMID: 19577810] (Feline)
• McEachern JA, Bingham J, Crameri G, Green DJ, Hancock TJ, Middleton D, Feng YR, Broder CC, Wang LF, Bossart KN A recombinant subunit vaccine formulation protects against lethal Nipah virus challenge in cats. Vaccine, 2008-06-02;26(31):3842-52. 2008-06-02 [PMID: 18556094] (Feline)

FAQs for IL-10 (DY736). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: IL10