Feline IFN-gamma DuoSet ELISA, 15 Plate

• Reactivity: Fe
• Applications: ELISA
• Conjugate: Biotin

Feline IFN-gamma DuoSet ELISA, 15 Plate Summary

• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: IFNG

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Feline IFN-gamma DuoSet ELISA, 15 Plate

• IFG
• IFI
• IFNG
• IFNgamma
• IFN-gamma
• Immune interferon
• interferon gamma
• interferon, gamma

Background

IFN-gamma (Interferon-gamma) is the prototype proinflammatory cytokine and is produced by a variety of immune cells under inflammatory conditions, notably by T cells and NK cells. It plays a key role in host defense by promoting the development and activation of Th1 cells, chemoattraction and activation of monocytes and macrophages, upregulation of antigen presentation molecules, and immunoglobulin class switching in B cells. It also exhibits antiviral, antiproliferative, and apoptotic effects. In addition, IFN-gamma functions as an anti-inflammatory mediator by promoting the development of regulatory T cells and inhibiting Th17 cell differentiation. IFN-gamma dimers signal through a receptor complex of two IFN-gamma R1 and two IFN-gamma R2 subunits.

Publications for IFN-gamma (DY764)(9)

Publications using DY764

• M Pearson, A LaVoy, S Evans, A Vilander, C Webb, B Graham, E Musselman, J LeCureux, S VandeWoude, GA Dean Mucosal Immune Response to Feline Enteric Coronavirus Infection Viruses, 2019-09-27;11(10):. 2019-09-27 [PMID: 31569783] (Feline)
• N Taechangam, SS Iyer, NJ Walker, B Arzi, DL Borjesson Mechanisms utilized by feline adipose-derived mesenchymal stem cells to inhibit T lymphocyte proliferation Stem Cell Res Ther, 2019-06-25;10(1):188. 2019-06-25 [PMID: 31238978] (Feline)
• W Wheat, L Chow, J Coy, E Contreras, M Lappin, S Dow Activation of upper respiratory tract mucosal innate immune responses in cats by liposomal toll-like receptor ligand complexes delivered topically J. Vet. Intern. Med., 2019-02-15;0(0):. 2019-02-15 [PMID: 30770582] (Feline)
• KC Clark, FA Fierro, EM Ko, NJ Walker, B Arzi, CG Tepper, H Dahlenburg, A Cicchetto, A Kol, L Marsh, WJ Murphy, N Fazel, DL Borjesson Human and feline adipose-derived mesenchymal stem cells have comparable phenotype, immunomodulatory functions, and transcriptome Stem Cell Res Ther, 2017-03-20;8(1):69. 2017-03-20 [PMID: 28320483] (Feline)
• Hsieh L, Chueh L Identification and genotyping of feline infectious peritonitis-associated single nucleotide polymorphisms in the feline interferon-gamma gene. Vet Res, 2014-05-21;45(0):57. 2014-05-21 [PMID: 24886103] (Feline)
• Rossi G, Cornaro C, Battilani M, Pocacqua V, Paltrinieri S Production of IFN-gamma in feline whole blood after incubation with potential T-cell epitopes of the nucleocapsid protein of feline coronavirus. Vet. Microbiol., 2011-02-13;150(3):248-56. 2011-02-13 [PMID: 21376481] (Feline)
• Wood BA, O'Halloran KP, Vandewoude S Development and validation of a multiplex microsphere-based assay for detection of domestic cat (Felis catus) cytokines. Clin. Vaccine Immunol., 2011-01-05;18(3):387-92. 2011-01-05 [PMID: 21209158] (Feline)
• Declue AE, Schooley E, Nafe LA, Reinero CR feG-COOH blunts eosinophilic airway inflammation in a feline model of allergic asthma. Inflamm. Res., 2009-03-03;58(8):457-62. 2009-03-03 [PMID: 19255722] (Feline)
• McEachern JA, Bingham J, Crameri G, Green DJ, Hancock TJ, Middleton D, Feng YR, Broder CC, Wang LF, Bossart KN A recombinant subunit vaccine formulation protects against lethal Nipah virus challenge in cats. Vaccine, 2008-06-02;26(31):3842-52. 2008-06-02 [PMID: 18556094] (Feline)

FAQs for IFN-gamma (DY764). (Showing 1 - 2 of 2 FAQs).

1. Is Recombinant Human IFN-gamma Protein - Clinical grade or preclinical ?
   • We are research use only.
2. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: IFNG