Recombinant Human FDX1 T7 Protein Summary
| Description |
A recombinant protein with a T7 tag and corresponding to the amino acids 61-184 of Human FDX1 Source: E.coli Amino Acid Sequence: MASMTGGQQM GRGSMSSSED KITVHFINRD GETLTTKGKV GDSLLDVVVE NNLDIDGFGA CEGTLACSTC HLIFEDHIYE KLDAITDEEN DMLDLAYGLT DRSRLGCQIC LTKSMDNMTV RVPETVADAR QSIDVGKTS |
| Source |
E. coli |
| Protein/Peptide Type |
Recombinant Protein |
| Gene |
FDX1 |
| Purity |
>90%, by SDS-PAGE |
| Endotoxin Note |
< 1.0 EU per 1 microgram of protein (determined by LAL method) |
Applications/Dilutions
| Dilutions |
|
| Theoretical MW |
15 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Packaging, Storage & Formulations
| Storage |
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer |
20 mM Tris-HCl buffer (pH8.0), 10% glycerol |
| Preservative |
No Preservative |
| Concentration |
1 mg/ml |
| Purity |
>90%, by SDS-PAGE |
Alternate Names for Recombinant Human FDX1 T7 Protein
Background
Ferredoxin1 is a small iron-sulfur protein that transfers electrons from NADPH through ferredoxin reductase to a terminal cytochrome P450. This particular oxidation/reduction system is found in steroidogenic tissues, and is involved with the synthesis of bile acid and vitamin D. It participates in the synthesis of thyroid hormones and transfers electrons from adrenodoxin reductase to the cholesterol side chain cleavage cytochrome P450. Recombinant human Ferredoxin 1 protein, fused to T7-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are
guaranteed for 3 months from date of receipt.
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