Flow Cytometry: Fc gamma RIIIA/CD16a Antibody (MEM-168) [Alexa Fluor (R) 488] [NB500-523AF488] - Analysis of CD16a in human buffy coat using anti-CD16a antibody AF488. The primary antibody was used at a dilution of ...read more
Hu, Po, PmSpecies Glossary
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Fc gamma RIIIA/CD16a Antibody (MEM-168) [Alexa Fluor (R) 488] Summary
The antibody MEM-168 reacts with CD16 antigen, a low affinity receptor for aggregated IgG (FcgammaRIII antigen). CD16 exists in two different isoforms: CD16a (FcgammaRIIIA; 50-65 kDa; expressed on NK-cells, monocytes and macrophages) and CD16b (FcgammaRIIIB; 48 kDa; mainly expressed on neutrophils).
Protein A or G purified
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FAQs for Fc gamma RIIIA/CD16a Antibody (NB500-523AF488). (Showing 1 - 1 of 1 FAQ).
I am interested in developing a surrogate ADCC assay (FC gamma III a binding assay) for which, I would require a CD16 Recombinant Protein as an antigen and Anti-Human IgG (Fc specific) as a secondary antibody as I am planning to perform this assay in an ELISA format. With this brief description, could you please suggest me the right CD16 Recombinant Protein among the three listed products in your web site (http://www.novusbio.com/proteins/cd16). Appreciate if you can share a reference article, where the use of this protein is cited.
The right CD16 protein for you depends on what sort of functionality you require for your assay. H00002214-P01 is made by a Taiwanese company called Abnova and we distribute it for them. They do not test the activity of their recombinant proteins. The proteins are produced in a cell-free wheat germ system with an N-terminal 26kDa GST-tag. Due to this expression system, in our experience, the proteins do not always undergo the same folding and post-transnational modifications they might undergo in an endogenous cell. Because of this, we do not recommend this protein for use in functional assays and do not guarantee them for this use. They are mainly intended for use in Western Blots and ELISA as a positive control with their antibody. NBP1-98908 is produced in E. coli and contains a His-tag and it also not guaranteed for functionality but may be due to its E. coli expression system. H00002214-G01 is also an Abnova and is made in their cell-free wheat germ expression system with proprietary liposome technology. This is a recently developed system by them and is intended to help the proteins fold and maintain functionality. Which protein would be best for you depends on what you require for your assay. Here is a link to our antibodies to the Fc region of human IgG. The best antibody for you will depend on what you would like to use for detection.