Fc gamma RIIIA/CD16a Antibody (CB16)

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow, ICC/IF
Clone
CB16
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

Fc gamma RIIIA/CD16a Antibody (CB16) Summary

Immunogen
Human CD16
Localization
Cell Surface
Specificity
It recognizes CD16 (Fcgamma RIII), the low-affinity receptor for IgG with an apparent molecular weight of 50-80 kDa. CD16 is represented by two similar genes, CD16A (Fcgamma RIIIA), which exists as a hetero-oligomeric polypeptide-anchored form in macrophages and NK cells and CD16B (Fcgamma RIIIB), which exist as a monomeric GPI-anchored form in neutrophils. Furthermore, there are two known polymorphisms of CD16B, NA-1 and NA-2. Individuals homozygous for NA-2 show a lower phagocytic capacity compared with NA-1. CD16 binds IgG in the form of immune complexes and shows preferential binding of IgG1 and IgG3 isotypes and minimal binding of IgG2 and IgG4. Upon IgG binding, both CD16 isoforms initiate signal transduction cascades that lead to a variety of responses including antibody-dependent cell-mediated cytotoxicity (ADCC), phagocytosis, degranulation and proliferation.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
FCGR3A
Purity
Protein A or G purified
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Applications/Dilutions

Dilutions
  • Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml
  • Immunofluorescence 0.5 - 1.0 ug/ml
Application Notes
Hu-chromosome location: 1q23
Molecular weight of antigen: 50-80kDa

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
10mM PBS and 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Purity
Protein A or G purified

Alternate Names for Fc gamma RIIIA/CD16a Antibody (CB16)

  • CD16a antigen
  • CD16a
  • CD16FCRIIIA
  • Fc fragment of IgG, low affinity IIIa, receptor (CD16a)
  • Fc fragment of IgG, low affinity IIIa, receptor for (CD16)
  • Fc gamma receptor III-A
  • Fc gamma RIIIA
  • FCG3
  • Fc-gamma receptor III-2 (CD 16)
  • Fc-gamma receptor IIIb (CD16)
  • Fc-gamma RIII
  • Fc-gamma RIIIa
  • Fc-gamma RIII-alpha
  • FCGR3
  • FCGR3A
  • FCGRIII
  • FcgRIIIA
  • FcR-10
  • FCRIII
  • FcRIIIa
  • IGFR3FcgammaRIIIA
  • immunoglobulin G Fc receptor III
  • low affinity III, receptor for (CD16)
  • low affinity immunoglobulin gamma Fc region receptor III-A
  • neutrophil-specific antigen NA

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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FAQs for Fc gamma RIIIA/CD16a Antibody (NBP2-44834). (Showing 1 - 1 of 1 FAQ).

  1. I am interested in developing a surrogate ADCC assay (FC gamma III a binding assay) for which, I would require a CD16 Recombinant Protein as an antigen and Anti-Human IgG (Fc specific) as a secondary antibody as I am planning to perform this assay in an ELISA format. With this brief description, could you please suggest me the right CD16 Recombinant Protein among the three listed products in your web site (http://www.novusbio.com/proteins/cd16). Appreciate if you can share a reference article, where the use of this protein is cited.
    • The right CD16 protein for you depends on what sort of functionality you require for your assay. H00002214-P01 is made by a Taiwanese company called Abnova and we distribute it for them. They do not test the activity of their recombinant proteins. The proteins are produced in a cell-free wheat germ system with an N-terminal 26kDa GST-tag. Due to this expression system, in our experience, the proteins do not always undergo the same folding and post-transnational modifications they might undergo in an endogenous cell. Because of this, we do not recommend this protein for use in functional assays and do not guarantee them for this use. They are mainly intended for use in Western Blots and ELISA as a positive control with their antibody. NBP1-98908 is produced in E. coli and contains a His-tag and it also not guaranteed for functionality but may be due to its E. coli expression system. H00002214-G01 is also an Abnova and is made in their cell-free wheat germ expression system with proprietary liposome technology. This is a recently developed system by them and is intended to help the proteins fold and maintain functionality. Which protein would be best for you depends on what you require for your assay. Here is a link to our antibodies to the Fc region of human IgG. The best antibody for you will depend on what you would like to use for detection.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-47951AF350
Alexa Fluor 405 NBP2-47951AF405
Alexa Fluor 488 NBP2-47951AF488
Alexa Fluor 532 NBP2-47951AF532
Alexa Fluor 594 NBP2-47951AF594
Alexa Fluor 647 NBP2-47951AF647
Alexa Fluor 700 NBP2-47951AF700
Alexa Fluor 750 NBP2-47951AF750
Allophycocyanin NBP2-47951APC
Allophycocyanin/Cy7 NBP2-47951APCCY7
Biotin NBP2-47951B
Biotin NBP2-47951B
DyLight 350 NBP2-47951UV
DyLight 405 NBP2-47951V
DyLight 488 NBP2-47951G
DyLight 550 NBP2-47951R
DyLight 594 NBP2-47951DL594
DyLight 650 NBP2-47951C
DyLight 680 NBP2-47951FR
DyLight 755 NBP2-47951IR
FITC NBP2-47951F
Janelia Fluor 549 NBP2-47951JF549
Janelia Fluor 646 NBP2-47951JF646
PE NBP2-47951PE
PE/Atto594 NBP2-47951PEATT594
PE/Cy5.5 NBP2-47951PECY55
PE/Cy7 NBP2-47951PECY7
PerCP NBP2-47951PCP

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Bioinformatics

Gene Symbol FCGR3A
Uniprot