![Western Blot: Fc gamma RIIB/CD32b Antibody [NBP2-14905] - Image from verified customer review.](http://images.novusbio.com/fullsize2/Fc-gamma-RIIB-CD32b-Antibody-Western-Blot-NBP2-14905-img0005.jpg "Western Blot: Fc gamma RIIB/CD32b Antibody [NBP2-14905] - Image from verified customer review.")
| Reactivity | Hu, RMSpecies Glossary |
| Applications | WB, Flow, ICC/IF |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human CD32B. The exact sequence is proprietary. |
| Predicted Species | Rhesus Macaque (92%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | FCGR2B |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | Flow cytometry/FACS Assay-dependent dilution |
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| Theoretical MW | 34 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Reviewed Applications |
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| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7), 20% Glycerol |
| Preservative | 0.01% Thimerosal |
| Purity | Immunogen affinity purified |
| Images | Ratings | Applications | Species | Date | Details | ||||||||
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reviewed by:
Anonymous |
WB | Human, Human cell line, mouse | 03/29/2019 |
Summary
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Secondary Antibodies |
Isotype Controls |
Diseases for Fc gamma RIIB/CD32b Antibody (NBP2-14905)Discover more about diseases related to Fc gamma RIIB/CD32b Antibody (NBP2-14905).
| Pathways for Fc gamma RIIB/CD32b Antibody (NBP2-14905)View related products by pathway.
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| Anonymous 03/29/2019 |
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| Application: | WB | |
| Species: | Human, Human cell line, mouse |
![Immunocytochemistry/Immunofluorescence: Fc gamma RIIB/CD32b Antibody [NBP2-14905] - Analysis of methanol-fixed A431, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize2/Fc-gamma-RIIB-CD32b-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-14905-img0003.jpg "Immunocytochemistry/Immunofluorescence: Fc gamma RIIB/CD32b Antibody [NBP2-14905] - Analysis of methanol-fixed A431, using antibody at 1:500 dilution.")
![Western Blot: Fc gamma RIIB/CD32b Antibody [NBP2-14905] - Sample (30 ug of whole cell lysate) A: THP-1 10% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize2/Fc-gamma-RIIB-CD32b-Antibody-Western-Blot-NBP2-14905-img0004.jpg "Western Blot: Fc gamma RIIB/CD32b Antibody [NBP2-14905] - Sample (30 ug of whole cell lysate) A: THP-1 10% SDS PAGE; antibody diluted at 1:1000.")
![Flow Cytometry: HLA DQ Antibody (SPV-L3) [NBP2-45041] - Flow Cytometric Analysis of Raji cells. HLA DQ Antibody (SPV-L3) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/images2/HLA DQ Antibody (SPV-L3)-Flow Cytometry-NBP2-45041-img0001.jpg)
![Western Blot: NOD2 Antibody (2D9) [NB100-524] - HCMV infection induces NOD2 mRNA and protein in HFFs and U373 cells. E. U373 glioma cells were infected with HCMV Towne strain and levels of NOD1, NOD2 and GAPDH mRNAs were measured by qRT-PCR at indicated time points. F. HFFs were infected with HCMV (Towne) at MOI of 1 PFU/cell and levels of NOD2 protein and B-actin were determined 48 and 72 hpi. G. HFFs were infected with HCMV (Towne) strain at MOI of 0.03 or 3 PFU/cell and levels of NOD2 protein and B-actin were determined at 48 hpi. Quantitative data represent mean values (+/-SD) of triplicate determinations from three independent experiments (*p<0.05, **p<0.01, ***p<0.001, one-way ANOVA test). Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0092704.g001) licensed under a CC-BY licence.](http://images.novusbio.com/images2/NOD2-Antibody-(2D9)-Western-Blot-NB100-524-img0018.jpg)
![Western Blot: NOD2 Antibody (2D9) [NB100-524] - Total protein from human THP1 and US-OS cells and mouse Raw264.7 cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-NOD2 in 1% non-fat milk in TBST and detected with an anti-mouse HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/images2/NOD2-Antibody-(2D9)-Western-Blot-NB100-524-img0013.jpg)
![Immunohistochemistry-Frozen: NOD2 Antibody (2D9) [NB100-524] - Overlay of NOD2-DyLight 488 (green) with phase contrast of murine colon. Image from verified customer review.](http://images.novusbio.com/images2/NOD2-Antibody-(2D9)-Immunohistochemistry-Frozen-NB100-524-img0016.jpg)
![Immunohistochemistry: CD11b Antibody [NB110-89474] - Staining of mouse pancreas using [NB110-89474] at 1:400 dilution. Nuclei counterstained with 4',6-diamidino-2-phenylindole (DAPI) (blue). Image from product review by verified customer.](http://images.novusbio.com/images2/CD11b-Antibody-Immunocytochemistry-Immunofluorescence-NB110-89474-img0010.jpg)
![Dual RNAscope ISH-IHC: CD11b Antibody [NB110-89474] - Formalin-fixed paraffin-embedded tissue sections of human lymph node were probed for CD11b mRNA (ACD RNAScope Probe, catalog #555098; Fast Red chromogen, ACD catalog # 322750). Adjacent tissue section was processed for immunohistochemistry using Rabbit Polyclonal (Novus Biologicals catalog #NB110-89474) at 1:50 dilution with 1 hour incubation at room temperature followed by incubation with anti-rabbit IgG VisUCyte HRP Polymer Antibody (Catalog # VC003) and DAB chromogen (yellow-brown). Tissue was counterstained with hematoxylin (blue). Specific staining was localized to lymphocytes.](http://images.novusbio.com/images2/CD11b-Antibody-Dual-RNAscope-ISH-IHC-NB110-89474-img0025.jpg)
![Immunohistochemistry: CD11b Antibody [NB110-89474] - Glibenclamide and Abcc8-/- suppress immune cell infiltration in EAE. White matter of lumbar spinal cord sections from WT control (a), untreated pid-30 WT/EAE (b), glibenclamide-treated pid-30 WT/EAE (c), and pid-30 Abcc8-/-/EAE (d) mice, stained with H&E or immunolabeled for CD45 (leucocyte), CD3 (T cells), CD20 (B cells), or CD11b (macrophage/microglia), as indicated; original magnification, x200 (H&E) or x400 (all immunolabelings). eleft panel: percent of quadrants with inflammatory cells on H&E; four mice/group. efour right panels: Quantification of CD-45-, CD3-, CD20-, and CD11b-expressing cells in white matter; four mice/group; ##P < 0.01 with respect to WT control; **P < 0.01, and ***P < 0.001 with respect to WT/EAE; scale bars, 100 um Image collected and cropped by CiteAb from the following publication (http://www.jneuroinflammation.com/content/12/1/210) licensed under a CC-BY licence.](http://images.novusbio.com/images2/CD11b-Antibody-Immunohistochemistry-NB110-89474-img0024.jpg)
![IL-4 [Unconjugated]](/sites/all/modules/enterprise-tech/et_datasheets/images/novus_guarantee.png "IL-4 [Unconjugated]")
![Western Blot: NDUFA2 Antibody [NBP2-93743] - Analysis of extracts of various cell lines, using NDUFA2 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.](http://images.novusbio.com/images2/NDUFA2 Antibody-Western Blot-NBP2-93743-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: NDUFA2 Antibody [NBP2-93743] - Analysis of NIH/3T3 cells using NDUFA2 at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/images2/NDUFA2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-93743-img0001.jpg)
![Immunohistochemistry-Paraffin: NDUFA2 Antibody [NBP2-93743] - Paraffin-embedded human liver cancer using NDUFA2.](http://images.novusbio.com/images2/NDUFA2-Antibody-Immunohistochemistry-Paraffin-NBP2-93743-img0003.jpg)
![Immunohistochemistry-Paraffin: CD35 Antibody (E11) [NBP2-29460] - Hhuman Tonsil stained with CD35 Antibody (E11).](http://images.novusbio.com/images2/CD35-Antibody-(E11)-Immunohistochemistry-Paraffin-NBP2-29460-img0003.jpg)
![Immunohistochemistry-Paraffin: CD35 Antibody (E11) [NBP2-29460] - Formalin-fixed, paraffin-embedded human tonsil stained with CD35 antibody at 1:200 using peroxidase-conjugate and DAB chromogen. Note membrane staining.](http://images.novusbio.com/images2/CD35-Antibody-(E11)-Immunohistochemistry-Paraffin-NBP2-29460-img0001.jpg)
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - An intracellular stain was performed on Raji cells with Adiponectin antibody NB100-65810 (blue) and a matched isotype control NBP2-24893 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by Dylight488-conjugated anti-rabbit secondary antibody. Image using the Azide Free form of this antibody.](http://images.novusbio.com/images2/Rabbit-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0006.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - An intracellular stain was performed on Raji cells with Adiponectin antibody NB100-65810 (blue) and a matched isotype control NBP2-24893 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by Dylight488-conjugated anti-rabbit secondary antibody. Image using the Azide Free form of this antibody.")