Fc gamma RIIB/CD32b Antibody (S07-4B5)

Western Blot: Fc gamma RIIB/CD32b Antibody (S07-4B5) [NBP3-19496] - Western blot detection of Fc gamma RIIB/CD32b in C6 cell lysates using NBP3-19496 (1:1000 diluted). Predicted band size: 34kDa. Observed band size: 44kDa.

Immunohistochemistry-Paraffin: Fc gamma RIIB/CD32b Antibody (S07-4B5) [NBP3-19496] - Immunohistochemistry of Fc gamma RIIB/CD32b in paraffin-embedded Human tonsil using NBP3-19496 at dilution 1/50

• Reactivity: Hu, Rt
• Applications: WB, IHC
• Clone: S07-4B5
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.3 mg/ml

Fc gamma RIIB/CD32b Antibody (S07-4B5) Summary

• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: A synthetic peptide of human Fc gamma RIIB/CD32b (Uniprot # P31994)
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: FCGR2B
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1/200
  • Immunohistochemistry-Paraffin
  • Western Blot 1/2000-1/10000
• Theoretical MW: 44 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% BSA
• Preservative: 0.01% Sodium Azide
• Concentration: 0.3 mg/ml
• Purity: Affinity purified

Alternate Names for Fc gamma RIIB/CD32b Antibody (S07-4B5)

• CD32 antigen
• CD32b
• CD32Fc fragment of IgG, low affinity II, receptor for (CD32)
• CDw32
• Fc fragment of IgG, low affinity IIb, receptor (CD32)
• Fc fragment of IgG, low affinity IIb, receptor for (CD32)
• Fc gamma RIIB
• FCG2
• Fc-gamma RII-b
• fc-gamma-RIIb
• FCGR2
• FCGR2B
• FcgRIIB
• FCRIIB
• FcRII-b
• IGFR2
• IgG Fc receptor II-b
• low affinity immunoglobulin gamma Fc region receptor II-b

Background

Low affinity IgG Fc receptor gamma II (Fc-gamma II), also known as CD32, is a member of the immunoreceptor tyrosine-based activating/inhibitory motif (ITAM/ITIM) family. Fc gamma receptors play a critical role in immunity by linking the IgG antibody mediated response with the regulatory functions of the immune system (1). Three classes of Fc gamma receptor exist; Fc gamma RI (CD64, a high affinity receptor), Fc gamma RII, and RIII (CD32 and CD16 respectively, both low affinity receptors). Fc gamma RIIa is an activating receptor, while RIIb1, RIIb2, and RIIb3 are inhibitory receptors generated by alternative splicing (1-2). Fc gamma RIIa is critical for antigen-presenting-cell activation and Fc gamma RIIb downregulates B-cell functions (3). Fc gamma RIIb has also been known to block the inflammatory immune response in mice (4). Once phosphorylated on tyrosine residues, Fc gamma RIIb associates with SHIP to downregulate phagocytosis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: FCGR2B