Fc gamma RIIA/CD32a Antibody [Unconjugated]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, B/N, CyTOF-ready
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

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Catalog# & Formulation Size Price

Fc gamma RIIA/CD32a Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human Fc gamma  RIIA/CD32a
Ala36-Ile218
Accession # AAA35827
Specificity
Detects human Fc gamma  RIIA/CD32a in direct ELISAs and Western blots. In Western blots, less than 5% cross-reactivity with recombinant human (rh) Fc gamma  RIIB and rhFc gamma  RIIIB is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
FCGR2A
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1 ug/mL
  • Flow Cytometry 2.5 ug/10^6 cells
  • Blockade of Receptor-ligand Interaction
  • CyTOF-ready
Reviewed Applications
Read 1 Review rated 3
using
AF1875 in the following applications:

Publications
Read Publications using
AF1875 in the following applications:

  • 1 publication
  • ICC
    1 publication
  • 5 publications
  • WB
    2 publications

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Fc gamma RIIA/CD32a Antibody [Unconjugated]

  • CD32 antigen
  • CD32a
  • CD32MGC23887
  • CDw32fc-gamma-RIIa
  • Fc fragment of IgG, low affinity IIa, receptor (CD32)
  • Fc fragment of IgG, low affinity IIa, receptor for (CD32)
  • Fc gamma RIIA
  • FCG2
  • Fc-gamma RII-a
  • Fc-gamma-RIIa
  • FcGR
  • FCGR2
  • FCGR2A
  • FCGR2A1
  • FcgRIIA
  • FCRIIA
  • fcRII-a
  • IGFR2MGC30032
  • IgG Fc receptor II-a
  • Immunoglobulin G Fc receptor II
  • low affinity immunoglobulin gamma Fc region receptor II-a

Background

Receptors for the Fc region of IgG (Fc gamma R) are members of the Ig superfamily that function in the activation or inhibition of immune responses. Three classes of human Fc gamma Rs: RI (CD64), RII (CD32), and RIII (CD16), which generate multiple isoforms, are recognized (1 - 3). The activating-type receptor either has or associates non-covalently with an accessory subunit (FcR gamma or zeta chain) that has an immunoreceptor tyrosine-based activation motif (ITAM) in its cytoplasmic domain. In contrast, the inhibitory receptor (Fc gamma RIIB) has a built-in immunoreceptor tyrosine-based inhibitory motif (ITIM) in its own cytoplasmic domain. Fc gamma RI is a
high-affinity receptor that binds monomeric IgG, both Fc gamma RII and RIII are low-affinity receptors that bind aggregated or immune complexed IgG (IC).

Three genes for human Fc gamma RII (A, B, and C) and one for mouse (Fc gamma RIIB), encoding type I transmembrane proteins with ITAM motifs (Fc gamma RII A and C) or ITIM motifs (Fc gamma RIIB) in their cytoplasmic domains, have been identified (1 - 3). The extracellular domain of human Fc gamma RIIA shares approximately 90% amino acid sequence homology with human Fc gamma RIIB and Fc gamma RIIC. Fc gamma RIIA is expressed on many immune cell types (macrophage, neutrophil, eosinophils, platelets, dendritic cells and Langerhan cells) where inhibitory ITIM-bearing receptors may also be coexpressed and co-engaged by specific ligands. Signaling through Fc gamma RIIA results in the initiation of inflammatory responses (cytolysis, phagocytosis, degranulation and cytokine production) that can be modulated by signals from the inhibitory receptors. The strength of the signal is dependent on the ratio of expression of the activating and inhibitory receptors. Besides IC, Fc gamma RII A also binds C-reactive protein (CRP) (4, 5). Two allelic variants (R167 and H167) of Fc gamma RIIA that differ in their ability to ligate human IgG2 or CRP exist. The H167 allele has been found to have a protective effect against lupus nephritis.

  1. van de Winkel, J. and P. Capes (1993) Immunol. Today 14:215.
  2. Ravetch, J.V. and S. Bolland (2001) Annu. Rev. Immunol. 19:275.
  3. Takai, T. (2002) Nature Rev. Immunol. 2:580.
  4. Chi, M. et al. (2002) J. Immunol. 168:1413.
  5. Zuniga, R. et al. (2003) Arthritis Rheum. 48:460.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Fc gamma RIIA/CD32a Antibody (AF1875)(9)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 4 applications: Flow, ICC, Neut, WB.


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Flow
(1)
ICC
(1)
Neut
(5)
WB
(2)
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Human
(9)
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Showing Publications 1 - 9 of 9.
Publications using AF1875 Applications Species
Hugo Tharinger Antibody-dependent and -independent uptake of HBsAg across human leukocyte subsets is similar between individuals with chronic hepatitis B Virus infection and healthy donors J. Viral Hepat, 2016;0(0):. 2016 [PMID: 28012213] (Flow,, Human) Flow Human
Tjiam M, Taylor J, Morshidi M, Sariputra L, Burrows S, Martin J, Deeks S, Tan D, Lee S, Fernandez S, French M Viremic HIV Controllers Exhibit High Plasmacytoid Dendritic Cell-Reactive Opsonophagocytic IgG Antibody Responses against HIV-1 p24 Associated with Greater Antibody Isotype Diversification. J Immunol, 2015;194(11):5320-8. 2015 [PMID: 25911748] (Neut,, Human) Neut Human
Crawford J, Pilling D, Gomer R FcgammaRI mediates serum amyloid P inhibition of fibrocyte differentiation. J Leukoc Biol, 2012;92(4):699-711. 2012 [PMID: 22493081] (ICC,, Human) ICC Human
Nakagawa T, Natsume A, Satoh M, Niwa R Nonfucosylated anti-CD20 antibody potentially induces apoptosis in lymphoma cells through enhanced interaction with FcgammaRIIIb on neutrophils. Leuk. Res., 2010;34(5):666-71. 2010 [PMID: 20022111] (Neut,, Human) Neut Human
Bournazos S, Hart SP, Chamberlain LH, Glennie MJ, Dransfield I Association of FcgammaRIIa (CD32a) with lipid rafts regulates ligand binding activity. J. Immunol., 2009;182(12):8026-36. 2009 [PMID: 19494328] (WB,, Human) WB Human
Marois L, Vaillancourt M, Marois S, Proulx S, Pare G, Rollet-Labelle E, Naccache PH The ubiquitin ligase c-Cbl down-regulates FcgammaRIIa activation in human neutrophils. J. Immunol., 2009;182(4):2374-84. 2009 [PMID: 19201892] (WB,, Human) WB Human
Montecucco F, Steffens S, Burger F, Pelli G, Monaco C, Mach F C-reactive protein (CRP) induces chemokine secretion via CD11b/ICAM-1 interaction in human adherent monocytes. J. Leukoc. Biol., 2008;84(4):1109-19. 2008 [PMID: 18591415] (Neut,, Human) Neut Human
Nabata A, Kuroki M, Ueba H, Hashimoto S, Umemoto T, Wada H, Yasu T, Saito M, Momomura S, Kawakami M C-reactive protein induces endothelial cell apoptosis and matrix metalloproteinase-9 production in human mononuclear cells: Implications for the destabilization of atherosclerotic plaque. Atherosclerosis, 2007;196(1):129-35. 2007 [PMID: 17531242] (Neut,, Human) Neut Human
Zhang R, Becnel L, Li M, Chen C, Yao Q C-reactive protein impairs human CD14+ monocyte-derived dendritic cell differentiation, maturation and function. Eur. J. Immunol., 2006;36(11):2993-3006. 2006 [PMID: 17051617] (Neut,, Human) Neut Human

Review for Fc gamma RIIA/CD32a Antibody (AF1875) (1) 31

Average Rating: 3
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using AF1875:
Filter by Applications
WB
(1)
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Human
(1)
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Images Ratings Applications Species Date Details
 
reviewed by:
Anonymous
WB Human 01/05/2015
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Summary

ApplicationWestern Blot
Sample TestedSee PMID 19372459
SpeciesHuman

Blocking

Blocking DetailsSee PMID 19372459

Details

Detection NotesSee PMID 19372459

Comments

CommentsPublished:See PMID 19372459

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FAQs for Fc gamma RIIA/CD32a Antibody (AF1875). (Showing 1 - 1 of 1 FAQs).

  1. Does any of your FCGR2A antibodies (19C10, 6D11, 13G9) cross react to FCGR2B?
    • For these antibodies, we have used the full-length protein FCGR2A (NP_067674) as the immunogen and we have not mapped the epitope that is detected with each Ab yet. I did an alignment of these two proteins and they are highly conserved thus it seems likely that there will be cross-reactivity.

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Anonymous
01/05/2015
Application: WB
Species: Human

Bioinformatics

Gene Symbol FCGR2A
Entrez
Uniprot