Fc gamma RII/CD32 Antibody [Unconjugated]

Whole blood granulocytes were stained with Goat Anti-Human Fc gamma RII/CD32 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1875, filled histogram) or isotype control antibody (Catalog # 4-001-A, open histogram) followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu
• Applications: WB, Flow, B/N, CyTOF-ready
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Fc gamma RII/CD32 Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Fc gamma RIIA/CD32a
  Ala36-Ile218
  Accession # AAA35827
• Specificity: Detects human Fc gamma RII/CD32 in direct ELISAs and Western blots. In Western blots, less than 5% cross-reactivity with recombinant human Fc gamma RIIIB is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: FCGR2A
• Purity Statement: Antigen Affinity-purified
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Blockade of Receptor-ligand Interaction
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
  *Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Fc gamma RII/CD32 Antibody [Unconjugated]

• CD32 antigen
• CD32B
• CD32Fc fragment of IgG, low affinity II, receptor for (CD32)
• CDw32
• Fc fragment of IgG, low affinity IIb, receptor (CD32)
• Fc fragment of IgG, low affinity IIb, receptor for (CD32)
• Fc gamma RII
• Fc gamma RIIb
• FCG2
• Fc-gamma RII-b
• FcgammaRIIb
• fc-gamma-RIIb
• FCGR2
• FcgRII
• FcgRIIB
• fcRII-b
• FcRII-c
• IGFR2
• IgG Fc receptor II-b
• low affinity immunoglobulin gamma Fc region receptor II-b

Background

Receptors for the Fc region of IgG (Fc gamma R) are members of the Ig superfamily that function in the activation or inhibition of immune responses. Three classes of human Fc gamma Rs: RI (CD64), RII (CD32), and RIII (CD16), which generate multiple isoforms, are recognized (1 - 3). The activating-type receptor either has or associates non-covalently with an accessory subunit (FcR gamma or zeta chain) that has an immunoreceptor tyrosine-based activation motif (ITAM) in its cytoplasmic domain. In contrast, the inhibitory receptor (Fc gamma RIIB) has a built-in immunoreceptor tyrosine-based inhibitory motif (ITIM) in its own cytoplasmic domain. Fc gamma RI is a
high-affinity receptor that binds monomeric IgG, both Fc gamma RII and RIII are low-affinity receptors that bind aggregated or immune complexed IgG (IC).

Three genes for human Fc gamma RII (A, B, and C) and one for mouse (Fc gamma RIIB), encoding type I transmembrane proteins with ITAM motifs (Fc gamma RII A and C) or ITIM motifs (Fc gamma RIIB) in their cytoplasmic domains, have been identified (1 - 3). The extracellular domain of human Fc gamma RIIA shares approximately 90% amino acid sequence homology with human Fc gamma RIIB and Fc gamma RIIC. Fc gamma RIIA is expressed on many immune cell types (macrophage, neutrophil, eosinophils, platelets, dendritic cells and Langerhan cells) where inhibitory ITIM-bearing receptors may also be coexpressed and co-engaged by specific ligands. Signaling through Fc gamma RIIA results in the initiation of inflammatory responses (cytolysis, phagocytosis, degranulation and cytokine production) that can be modulated by signals from the inhibitory receptors. The strength of the signal is dependent on the ratio of expression of the activating and inhibitory receptors. Besides IC, Fc gamma RII A also binds C-reactive protein (CRP) (4, 5). Two allelic variants (R167 and H167) of Fc gamma RIIA that differ in their ability to ligate human IgG2 or CRP exist. The H167 allele has been found to have a protective effect against lupus nephritis.

1. van de Winkel, J. and P. Capes (1993) Immunol. Today 14:215.
2. Ravetch, J.V. and S. Bolland (2001) Annu. Rev. Immunol. 19:275.
3. Takai, T. (2002) Nature Rev. Immunol. 2:580.
4. Chi, M. et al. (2002) J. Immunol. 168:1413.
5. Zuniga, R. et al. (2003) Arthritis Rheum. 48:460.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Fc gamma RII/CD32 Antibody (AF1875)(12)

Publications using AF1875

• LA Henze, TTD Luong, B Boehme, J Masyout, MP Schneider, S Brachs, F Lang, B Pieske, A Pasch, KU Eckardt, J Voelkl, I Alesutan Impact of C-reactive protein on osteo-/chondrogenic transdifferentiation and calcification of vascular smooth muscle cells Aging (Albany NY), 2019-08-03;11(15):5445-5462. 2019-08-03 [PMID: 31377747] (Western Blot, Human)
• Tomas Castro-Dopico, Thomas W. Dennison, John R. Ferdinand, Rebeccah J. Mathews, Aaron Fleming, Dean Clift et al. Anti-commensal IgG Drives Intestinal Inflammation and Type 17 Immunity in Ulcerative Colitis Immunity 4/16/2019 [PMID: 30876876]
• Hugo Tharinger Antibody-dependent and -independent uptake of HBsAg across human leukocyte subsets is similar between individuals with chronic hepatitis B Virus infection and healthy donors J. Viral Hepat, 2017-02-01;0(0):. 2017-02-01 [PMID: 28012213] (Flow Cytometry, Human)
• Tjiam M, Taylor J, Morshidi M, Sariputra L, Burrows S, Martin J, Deeks S, Tan D, Lee S, Fernandez S, French M Viremic HIV Controllers Exhibit High Plasmacytoid Dendritic Cell-Reactive Opsonophagocytic IgG Antibody Responses against HIV-1 p24 Associated with Greater Antibody Isotype Diversification. J Immunol, 2015-04-24;194(11):5320-8. 2015-04-24 [PMID: 25911748] (Neutralization, Human)
• Crawford J, Pilling D, Gomer R FcgammaRI mediates serum amyloid P inhibition of fibrocyte differentiation. J Leukoc Biol, 2012-04-09;92(4):699-711. 2012-04-09 [PMID: 22493081] (ICC, Human)
• Margaret E. Ackerman, Brian Moldt, Richard T. Wyatt, Anne-Sophie Dugast, Elizabeth McAndrew, Stephen Tsoukas et al. A robust, high-throughput assay to determine the phagocytic activity of clinical antibody samples Journal of Immunological Methods 3/7/2011 [PMID: 21192942]
• Nakagawa T, Natsume A, Satoh M, Niwa R Nonfucosylated anti-CD20 antibody potentially induces apoptosis in lymphoma cells through enhanced interaction with FcgammaRIIIb on neutrophils. Leuk. Res., 2010-05-01;34(5):666-71. 2010-05-01 [PMID: 20022111] (Neutralization, Human)
• Bournazos S, Hart SP, Chamberlain LH, Glennie MJ, Dransfield I Association of FcgammaRIIa (CD32a) with lipid rafts regulates ligand binding activity. J. Immunol., 2009-06-15;182(12):8026-36. 2009-06-15 [PMID: 19494328] (Western Blot, Human)
• Marois L, Vaillancourt M, Marois S, Proulx S, Pare G, Rollet-Labelle E, Naccache PH The ubiquitin ligase c-Cbl down-regulates FcgammaRIIa activation in human neutrophils. J. Immunol., 2009-02-15;182(4):2374-84. 2009-02-15 [PMID: 19201892] (Western Blot, Human)
• Montecucco F, Steffens S, Burger F, Pelli G, Monaco C, Mach F C-reactive protein (CRP) induces chemokine secretion via CD11b/ICAM-1 interaction in human adherent monocytes. J. Leukoc. Biol., 2008-06-30;84(4):1109-19. 2008-06-30 [PMID: 18591415] (Neutralization, Human)
• Nabata A, Kuroki M, Ueba H, Hashimoto S, Umemoto T, Wada H, Yasu T, Saito M, Momomura S, Kawakami M C-reactive protein induces endothelial cell apoptosis and matrix metalloproteinase-9 production in human mononuclear cells: Implications for the destabilization of atherosclerotic plaque. Atherosclerosis, 2007-05-24;196(1):129-35. 2007-05-24 [PMID: 17531242] (Neutralization, Human)
• Zhang R, Becnel L, Li M, Chen C, Yao Q C-reactive protein impairs human CD14+ monocyte-derived dendritic cell differentiation, maturation and function. Eur. J. Immunol., 2006-11-01;36(11):2993-3006. 2006-11-01 [PMID: 17051617] (Neutralization, Human)

Review for Fc gamma RII/CD32 Antibody (AF1875) (1)

Average Rating: 3

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer WB Human 01/05/2015

Summary

• Application: Western Blot
• Sample Tested: See PMID 19372459
• Species: Human

Bioinformatics

• Gene Symbol: FCGR2A
• Uniprot:
  • AAA35827()