Fc gamma RIIA/CD32a Antibody (3L1) - Azide and BSA Free

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, CyTOF-ready
Clone
3L1
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
LYOPH

Order Details

Fc gamma RIIA/CD32a Antibody (3L1) - Azide and BSA Free Summary

Immunogen
Human recombinant CD32 EC domain
Isotype
IgG2
Clonality
Monoclonal
Host
Mouse
Gene
FCGR2A
Purity
Protein G purified
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Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 1:10 - 1:1000
  • Western Blot 1:500 - 1:1000
Application Notes
This antibody is Cytof ready.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
No Preservative
Concentration
LYOPH
Purity
Protein G purified
Reconstitution Instructions
Centrifuge vial prior to opening. Reconstitute the antibody with 500 ul sterile PBS and the final concentration is 200 ug/ml.

Alternate Names for Fc gamma RIIA/CD32a Antibody (3L1) - Azide and BSA Free

  • CD32 antigen
  • CD32a
  • CD32MGC23887
  • CDw32fc-gamma-RIIa
  • Fc fragment of IgG, low affinity IIa, receptor (CD32)
  • Fc fragment of IgG, low affinity IIa, receptor for (CD32)
  • Fc gamma RIIA
  • FCG2
  • Fc-gamma RII-a
  • Fc-gamma-RIIa
  • FcGR
  • FCGR2
  • FCGR2A
  • FCGR2A1
  • FcgRIIA
  • FCRIIA
  • fcRII-a
  • IGFR2MGC30032
  • IgG Fc receptor II-a
  • Immunoglobulin G Fc receptor II
  • low affinity immunoglobulin gamma Fc region receptor II-a

Background

The FCGR2A gene encodes a low affinity immunoglobulin gamma Fc region receptor II-a protein that in isoform 1 is 317 amino acids long at 35 kDA and at isoform 2 is 316 amino acids long at nearly 35 kDA. These proteins are found on the surface of many immune system response cells that act as a low affinity receptor through encouraging responses against pathogens and soluble antigens. Additionally, it functions to initiate phagocytosis of opsonized antigens. FCGR2A participates in Fc-GammaR pathway, osteoclast differentiation, signaling events controlled by PTP1B, and NFAT in immune response. It is known to interact with genes SYK, FYN, HCK, LGALS3, and PIK3R1. FCGR2A is linked to lupus, thrombocytopenia, b-cell lymphomas, otitis media, adult-onset still's disease, guillain-barre syndrome, asymptomatic dengue, severe acute respiratory syndrome.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for Fc gamma RIIA/CD32a Antibody (NBP2-35936). (Showing 1 - 1 of 1 FAQ).

  1. Does any of your FCGR2A antibodies (19C10, 6D11, 13G9) cross react to FCGR2B?
    • For these antibodies, we have used the full-length protein FCGR2A (NP_067674) as the immunogen and we have not mapped the epitope that is detected with each Ab yet. I did an alignment of these two proteins and they are highly conserved thus it seems likely that there will be cross-reactivity.

Secondary Antibodies

 

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Bioinformatics Tool for Fc gamma RIIA/CD32a Antibody (NBP2-35936)

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Diseases for Fc gamma RIIA/CD32a Antibody (NBP2-35936)

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Bioinformatics

Gene Symbol FCGR2A
Uniprot