Fc gamma RI/CD64 Antibody (OTI1A8) - Azide and BSA Free

Western Blot: Fc gamma RI/CD64 Antibody (OTI1A8) - Azide and BSA Free [NBP2-70702] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Fc gamma RI/CD64.

Immunocytochemistry/Immunofluorescence: Fc gamma RI/CD64 Antibody (OTI1A8) - Azide and BSA Free [NBP2-70702] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY Fc gamma RI/CD64.

Immunohistochemistry: Fc gamma RI/CD64 Antibody (OTI1A8) - Azide and BSA Free [NBP2-70702] - Analysis of Carcinoma of Human prostate tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Flow Cytometry: Fc gamma RI/CD64 Antibody (OTI1A8) - Azide and BSA Free [NBP2-70702] - analysis of living 293T cells transfected with FCGR1A overexpression plasmid .

Immunohistochemistry: Fc gamma RI/CD64 Antibody (OTI1A8) - Azide and BSA Free [NBP2-70702] - Analysis of Adenocarcinoma of Human endometrium tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Immunohistochemistry: Fc gamma RI/CD64 Antibody (OTI1A8) - Azide and BSA Free [NBP2-70702] - Analysis of Human lymphoma tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

• Reactivity: Hu
• Applications: WB, Flow, ICC/IF, IHC
• Clone: OTI1A8
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

Fc gamma RI/CD64 Antibody (OTI1A8) - Azide and BSA Free Summary

• Immunogen: Full length human recombinanti protein of human FCGR1A(NP_000557) produced in HEK293T cell.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: FCGR1A
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin
  • Western Blot 1:2000
• Theoretical MW: 42.5 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from PBS (pH 7.3) with 8% Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for Fc gamma RI/CD64 Antibody (OTI1A8) - Azide and BSA Free

• CD64 antigen
• CD64
• CD64a
• Fc fragment of IgG, high affinity Ia, receptor (CD64)
• Fc gamma RI
• FCG1
• Fc-gamma receptor I B2
• Fc-gamma RI
• Fc-gamma RIA
• FcgammaRIa
• FCGR1
• FcgRI
• FcgRIA
• FCRI
• FcRIA
• FLJ18345
• high affinity Ia, receptor for (CD64)
• high affinity immunoglobulin gamma Fc receptor I
• IgG Fc receptor I

Background

CD64 is a 72 kD single chain type I glycoprotein also known as Fc gammaRI and FcR I. CD64 is a member of the immunoglobulin superfamily and is expressed on monocytes/macrophages, dendritic cells, and activated granulocytes. The expression can be upregulated by IFN- gamma stimulation. CD64 binds IgG immune complex. It plays a role in antigen capture, phagocytosis of IgG/antigen complexes, and antibody-dependent cellular cytotoxicity (ADCC).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: FCGR1A