Fc gamma RI/CD64 Antibody (OTI1A8)

Western Blot: Fc gamma RI/CD64 Antibody (OTI1A8) [NBP2-45625] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Fc gamma RI/CD64.

Immunocytochemistry/Immunofluorescence: Fc gamma RI/CD64 Antibody (OTI1A8) [NBP2-45625] - Fc gamma RI/CD64 Antibody (1A8) [NBP2-45625] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY Fc gamma RI/CD64.

Immunohistochemistry: Fc gamma RI/CD64 Antibody (OTI1A8) [NBP2-45625] - Analysis of Adenocarcinoma of Human endometrium tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Flow Cytometry: Fc gamma RI/CD64 Antibody (OTI1A8) [NBP2-45625] - analysis of living 293T cells transfected with FCGR1A overexpression plasmid .

Immunohistochemistry: Fc gamma RI/CD64 Antibody (OTI1A8) [NBP2-45625] - Analysis of Human lymphoma tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Immunohistochemistry: Fc gamma RI/CD64 Antibody (OTI1A8) [NBP2-45625] - Analysis of Carcinoma of Human prostate tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

• Reactivity: Hu
• Applications: WB, Flow, ICC/IF, IHC
• Clone: OTI1A8
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

Fc gamma RI/CD64 Antibody (OTI1A8) Summary

• Description: Novus Biologicals Mouse Fc gamma RI/CD64 Antibody (OTI1A8) (NBP2-45625) is a monoclonal antibody validated for use in IHC, WB, Flow and ICC/IF. Anti-Fc gamma RI/CD64 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Full length human recombinanti protein of human FCGR1A(NP_000557) produced in HEK293T cell.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: FCGR1A
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
  • Western Blot 1:2000
• Theoretical MW: 42.5 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 1.0% BSA and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Fc gamma RI/CD64 Antibody (OTI1A8)

• CD64 antigen
• CD64
• CD64a
• Fc fragment of IgG, high affinity Ia, receptor (CD64)
• Fc gamma RI
• FCG1
• Fc-gamma receptor I B2
• Fc-gamma RI
• Fc-gamma RIA
• FcgammaRIa
• FCGR1
• FcgRI
• FcgRIA
• FCRI
• FcRIA
• FLJ18345
• high affinity Ia, receptor for (CD64)
• high affinity immunoglobulin gamma Fc receptor I
• IgG Fc receptor I

Background

CD64 is a 72 kD single chain type I glycoprotein also known as Fc gammaRI and FcR I. CD64 is a member of the immunoglobulin superfamily and is expressed on monocytes/macrophages, dendritic cells, and activated granulocytes. The expression can be upregulated by IFN- gamma stimulation. CD64 binds IgG immune complex. It plays a role in antigen capture, phagocytosis of IgG/antigen complexes, and antibody-dependent cellular cytotoxicity (ADCC).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Fc gamma RI/CD64 Antibody (NBP2-45625)(1)

Publication using NBP2-45625

• Low JT, Chandramohan V, Bowie ML et al. Epigenetic STING silencing is developmentally conserved in gliomas and can be rescued by methyltransferase inhibition Cancer cell 2022-05-09 [PMID: 35487217] (IHC-P, Human)

Bioinformatics

• Gene Symbol: FCGR1A