Fc gamma RI/CD64 Antibodies

MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins." class="big_lightbox"> MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins." alt="Human peripheral blood monocytes were stained with Mouse Anti-Human Fc gamma RI/CD64 Monoclonal Antibody (Catalog # MAB1257, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins." class="big_thumb" />

NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_lightbox"> NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_thumb" />

HAF017). A specific band was detected for Fc gamma RI/CD64 at approximately 60-75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox"> HAF017). A specific band was detected for Fc gamma RI/CD64 at approximately 60-75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of RAW 264.7 mouse monocyte/macrophage cell line and J774A.1 mouse reticulum cell sarcoma macrophage cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Mouse Fc gamma RI/CD64 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2074) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Fc gamma RI/CD64 at approximately 60-75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

MAB002, open histogram), followed by PE-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins." class="big_lightbox"> MAB002, open histogram), followed by PE-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins." alt="Human peripheral blood monocytes were stained with Mouse Anti-Human Fc gamma RI/CD64 Monoclonal Antibody (Catalog # MAB12572, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by PE-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins." class="big_thumb" />

MAB002, open histogram) followed by Phycoerthyrin-conjugated anti-Mouse IgG Secondary Antibody (F0102B). Staining was performed using our Staining Membrane-associated Proteins protocol." class="big_lightbox"> MAB002, open histogram) followed by Phycoerthyrin-conjugated anti-Mouse IgG Secondary Antibody (F0102B). Staining was performed using our Staining Membrane-associated Proteins protocol." class="big_thumb" />

MAB002, open histogram) followed by Phycoerythrin-conjugated anti-Mouse IgG Secondary Antibody (F0102B). No staining in the Fc gamma RI/CD64 knockout U937 cell line was observed. Staining was performed using our Staining Membrane-associated Proteins protocol. " class="big_lightbox"> MAB002, open histogram) followed by Phycoerythrin-conjugated anti-Mouse IgG Secondary Antibody (F0102B). No staining in the Fc gamma RI/CD64 knockout U937 cell line was observed. Staining was performed using our Staining Membrane-associated Proteins protocol. " class="big_thumb" />

HAF005). A specific band was detected for Fc gamma RI/CD64 at approximately 60-75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox"> HAF005). A specific band was detected for Fc gamma RI/CD64 at approximately 60-75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of RAW 264.7 mouse monocyte/macrophage cell line and J774A.1 mouse reticulum cell sarcoma macrophage cell line. PVDF membrane was probed with 2 µg/mL of Rat Anti-Mouse Fc gamma RI/CD64 Monoclonal Antibody (Catalog # MAB2074) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Fc gamma RI/CD64 at approximately 60-75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins." class="big_lightbox"> MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins." alt="Human peripheral blood monocytes were stained with Mouse Anti-Human Fc gamma RI/CD64 Monoclonal Antibody (Catalog # MAB12573, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins." class="big_thumb" />

VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in lymphocytes. Staining was performed using our IHC Staining with VisUCyte HRP Polymer Detection Reagents protocol." class="big_lightbox"> VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in lymphocytes. Staining was performed using our IHC Staining with VisUCyte HRP Polymer Detection Reagents protocol." alt="Fc gamma  RI/CD64 was detected in immersion fixed paraffin-embedded sections of human spleen tissue using Mouse Anti-Human Fc gamma  RI/CD64 Monoclonal Antibody (Catalog # MAB12574) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in lymphocytes. Staining was performed using our IHC Staining with VisUCyte HRP Polymer Detection Reagents protocol." class="big_thumb" />