Fatty Acid Synthase/FASN Antibody (JJ0939)

Western Blot: Fatty Acid Synthase/FASN Antibody (JJ0939) [NBP2-66833] - Analysis of Fatty Acid Synthase on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: MCF-7 cell lysateLane 2: SW480 cell lysate

Immunocytochemistry/Immunofluorescence: Fatty Acid Synthase/FASN Antibody (JJ0939) [NBP2-66833] - Analysis of SK-Br-3 cells labeling Fatty Acid Synthase/FASN with Rabbit anti-Fatty Acid Synthase antibody (permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Fatty Acid Synthase antibody was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

Immunohistochemistry-Paraffin: Fatty Acid Synthase/FASN Antibody (JJ0939) [NBP2-66833] - Analysis of paraffin-embedded mouse colon tissue using anti-Fatty Acid Synthase antibody. Counter stained with hematoxylin.

Flow Cytometry: Fatty Acid Synthase/FASN Antibody (JJ0939) [NBP2-66833] - Analysis of A549 cells with Fatty Acid Synthase antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody

Immunocytochemistry/Immunofluorescence: Fatty Acid Synthase/FASN Antibody (JJ0939) [NBP2-66833] - Staining Fatty Acid Synthase in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: Fatty Acid Synthase/FASN Antibody (JJ0939) [NBP2-66833] - Staining Fatty Acid Synthase in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: Fatty Acid Synthase/FASN Antibody (JJ0939) [NBP2-66833] - Staining Fatty Acid Synthase in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: Fatty Acid Synthase/FASN Antibody (JJ0939) [NBP2-66833] - Staining Fatty Acid Synthase in RH-35 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: Fatty Acid Synthase/FASN Antibody (JJ0939) [NBP2-66833] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-Fatty Acid Synthase antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: Fatty Acid Synthase/FASN Antibody (JJ0939) [NBP2-66833] - Analysis of paraffin-embedded human liver tissue using anti-Fatty Acid Synthase antibody. Counter stained with hematoxylin.

Product Discontinued

Fatty Acid Synthase/FASN Antibody (JJ0939) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within C-terminal human Fatty Acid Synthase/FASN. (SwissProt: P49327 Human; SwissProt: P19096 Mouse; SwissProt: P12785 Rat)
• Localization: Cytoplasm, Melanosome.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: FASN
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:100-1:500
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Immunoprecipitation
  • Western Blot 1:1000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Fatty Acid Synthase/FASN Antibody (JJ0939)

• EC 2.3.1
• EC 2.3.1.85
• EC 6.3.3.1
• FASN
• FASshort chain dehydrogenase/reductase family 27X, member 1
• Fatty Acid Synthase
• MGC14367
• MGC15706
• OA-519
• SDR27X1

Background

Fatty Acid Synthase (FAS) is a very large enzymatic system involved in de novo lipogenesis (triglyceride production). Specifically, FAS catalyzes the synthesis of palmitate from acetyl-CoA and malonyl-CoA, in the presence of NADPH, into long-chain saturated fatty acids. It is also one of the accepted markers for insulin resistance and SREBP, LXR activation.

In some cancer cell lines, FAS has been found to be fused with ER-alpha. Therefore, FAS antibodies are useful tools for studies on fatty acid synthesis and research on certian cancers.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: FASN