Fatty Acid Synthase/FASN Antibody (647114) [Alexa Fluor® 350]

• Reactivity: Hu
• Applications: WB
• Clone: 647114
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 350

Fatty Acid Synthase/FASN Antibody (647114) [Alexa Fluor® 350] Summary

• Immunogen: E. coli-derived recombinant human Fatty Acid Synthase/FASN
  Ser10-Cys212
  Accession # P49327
• Specificity: Detects Fatty Acid Synthase/FASN in direct ELISAs and Western blots. In direct ELISAs, approximately 25% cross-reactivity with recombinant mouse (rm) Ephrin-A2 is observed and no cross-reactivity with recombinant human (rh) Ephrin
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Fatty Acid Synthase/FASN Antibody (647114) [Alexa Fluor® 350]

• EC 2.3.1
• EC 2.3.1.85
• EC 6.3.3.1
• FASN
• FASshort chain dehydrogenase/reductase family 27X, member 1
• Fatty Acid Synthase
• MGC14367
• MGC15706
• OA-519
• SDR27X1

Background

FASN (Fatty Acid Synthase; also FAS) is a cytosolic 270-280 kDa member of the short chain dehydrogenase/reductase family 27X. It is expressed in neurons, skeletal muscle, adipocytes and hepatocytes, and both catalyzes the formation of palmitic acid from acetylCoA and malonylCoA, and likely mediates the transfer of fatty acids to peptides. Human FASN is 2511 amino acids (aa) in length ands contains multiple domains, including a  beta -ketoacyl synthase domain (aa 1-414), an acyl and malonyl transferase domain (aa 429-817), an enoyl reductase domain (aa 1563-1863), a beta -ketoacyl reductase domain (aa 1864-2118) and a thioesterase domain (aa 2207‑2511). FASN exists as an antiparallel homodimer. There is one theoretical alternative start site at Met2073. Over aa 9-212, human FASN shares 92% aa identity with mouse FASN.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.