Fascin Antibody - BSA Free

Western Blot: Fascin Antibody [NBP1-91887] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and Fascin knockout (KO) HeLa cell line. PVDF membrane was probed with 0.4 ug/ml of Rabbit Anti-Human Fascin Polyclonal Antibody (Catalog # NBP1-91887) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for Fascin at approximately 55 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.

Western Blot: Fascin Antibody [NBP1-91887] - Analysis in human cell lines PC-3 and SK-MEL-30 using anti-FSCN1 antibody. Corresponding FSCN1 RNA-seq data are presented for the same cell lines. Loading control: anti-GAPDH.

Immunohistochemistry-Paraffin: Fascin Antibody [NBP1-91887] - Staining of human cerebellum, cerebral cortex, testis and tonsil using Anti-Fascin antibody NBP1-91887 (A) shows similar protein distribution across tissues to independent antibody NBP2-58042 (B).

Immunocytochemistry/Immunofluorescence: Fascin Antibody [NBP1-91887] - Staining of human cell line A-431 shows localization to cytosol. Antibody staining is shown in green.

Immunohistochemistry-Paraffin: Fascin Antibody [NBP1-91887] - Staining of human tonsil shows strong cytoplasmic positivity in non-germinal center cells.

Western Blot: Fascin Antibody [NBP1-91887] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.

Immunohistochemistry-Paraffin: Fascin Antibody [NBP1-91887] - Staining of human cerebellum shows strong cytoplasmic positivity in cells in molecular layer.

Immunohistochemistry-Paraffin: Fascin Antibody [NBP1-91887] - Staining of human cerebral cortex shows strong cytoplasmic positivity in glial cells.

Immunohistochemistry-Paraffin: Fascin Antibody [NBP1-91887] - Staining of human testis shows strong cytoplasmic positivity in cells in seminiferous ducts.

Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC, Mycoplasma
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Fascin Antibody - BSA Free Summary

• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: GKDELFALEQSCAQVVLQAANERNVSTRQGMDLSANQDEETDQETFQLEIDRDTKKCAFRTHTGKYWTLTATGGVQSTASSKNASCYFDIEWRDRRITLRASNGKFVTSKKNGQ
• Marker: Dendritic Cell Marker
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: FSCN1
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Knockdown Validated
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
• Control Peptide:

  Fascin Protein (NBP1-91887PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for Fascin Antibody - BSA Free

• FAN1
• FAN1,55 kDa actin-bundling protein
• fascin homolog 1, actin-bundling protein (Strongylocentrotus purpuratus)
• Fascin
• FLJ38511
• FSCN1
• HSN
• p55actin bundling protein
• singed (Drosophila)-like (sea urchin fascin homolog like)
• singed-like (fascin homolog, sea urchin)
• Singed-like Protein
• SNL
• SNLfascin

Background

Organizes filamentous actin into bundles with a minimum of 4.1:1 actin/fascin ratio. Probably involved in the assembly of actin filament bundles present in microspikes, membrane ruffles, and stress fibers

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: FSCN1