Fascin Antibody (FSCN1/417)

Western Blot: Fascin Antibody (FSCN1/417) [NBP2-44383] - HeLa Cell Lysate using Fascin-1 Monoclonal Antibody (FSN1/417)

Immunohistochemistry-Paraffin: Fascin Antibody (FSCN1/417) [NBP2-44383] - Human Testicular Carcinoma stained with Fascin-1 Monoclonal Antibody (FSCN1/417)

Immunohistochemistry-Paraffin: Fascin Antibody (FSCN1/417) [NBP2-44383] - Human Hodgkin's Lymphoma stained with Fascin-1 Monoclonal Antibody (FSCN1/417)

Immunohistochemistry-Paraffin: Fascin Antibody (FSCN1/417) [NBP2-44383] - Human Ovarian Carcinoma stained with Fascin-1 Monoclonal Antibody (FSCN1/417)

Simple Western: Fascin Antibody (FSCN1/417) [NBP2-44383] - Simple Western lane view shows a specific band for Fascin in 0.2 mg/ml of HeLa lysate(s). This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Simple Western: Fascin Antibody (FSCN1/417) [NBP2-44383] - Electropherogram image of the corresponding Simple Western lane. Fascin antibody was used at 10 ug/ml dilution of HeLa lysates(s) respectively.

• Reactivity: Hu
• Applications: WB, Simple Western, Flow, IHC-P, PAGE, ICC/IF
• Clone: FSCN1/417
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

Fascin Antibody (FSCN1/417) Summary

• Immunogen: Full length recombinant human FSCN1 protein
• Localization: Cytoplasmic
• Marker: Dendritic Cell Marker
• Specificity: Recognizes a protein of 55kDa, which is identified as fascin-1. Its actin binding ability is regulated by phosphorylation. Antibody to fascin-1 is a very sensitive marker for Reed-Sternberg cells and variants in nodular sclerosis, mixed cellularity, and lymphocyte depletion Hodgkin's disease. It is uniformly negative in lymphoid cells, plasma cells, and myeloid cells. Fascin-1 is also expressed in dendritic cells. This marker may be helpful to distinguish between Hodgkin lymphoma and non-Hodgkin lymphoma in difficult cases. Also, the lack of expression of fascin-1 in the neoplastic follicles in follicular lymphoma may be helpful in distinguishing these lymphomas from reactive follicular hyperplasia in which the number of follicular dendritic cells is normal or increased. Antibody to fascin-1 has been suggested as a prognostic marker in neuroendocrine neoplasms of the lung as well as in ovarian cancer. Fascin-1 expression may be induced by Epstein-Barr virus (EBV) infection of B cells with the possibility that viral induction of fascin in lymphoid or other cell types must also be considered in EBV-positive cases.
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: FSCN1
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.5 - 1.0 ug/ml
  • Simple Western 10 ug/ml
  • Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml
  • Immunohistochemistry-Paraffin 0.5 - 1.0 ug/ml
  • SDS-Page
  • Immunofluorescence 1 - 2 ug/ml
• Application Notes: Hu-chromosome location: 7p22.1 Immunohistochemistry-Paraffin 0.5 - 1 ug/ml for 30 minutes at RT; Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10 - 20 min followed by cooling at RT for 20 minutes.
  
  In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size.
• Theoretical MW: 55 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  Fascin Knockout HeLa Cell Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 10mM PBS and 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Protein A or G purified

Alternate Names for Fascin Antibody (FSCN1/417)

• FAN1
• FAN1,55 kDa actin-bundling protein
• fascin homolog 1, actin-bundling protein (Strongylocentrotus purpuratus)
• Fascin
• FLJ38511
• FSCN1
• HSN
• p55actin bundling protein
• singed (Drosophila)-like (sea urchin fascin homolog like)
• singed-like (fascin homolog, sea urchin)
• Singed-like Protein
• SNL
• SNLfascin

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: FSCN1
• Uniprot:
  • Q16658(Human)