FANCG Antibody - Azide and BSA Free

Western Blot: FANCG Antibody [NB100-2566] - Detection of FANC G in transfected COS1 cell lysate.

• Reactivity: Hu
• Applications: WB, WB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

FANCG Antibody - Azide and BSA Free Summary

• Immunogen: A peptide corresponding to the C-terminus of human FANCG (within amino acids 550-622). [SwissProt# O15287]
• Localization: The major form is nuclear. The minor form is cytoplasmic.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: FANCG
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • Immunoblotting reported in scientific literature (PMID 24910428)
  • Western Blot 1:1000
• Application Notes: In Western Blot, a band is seen at approximately 69 kDa. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Theoretical MW: 69 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  FANCG Overexpression Lysate

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: mouse (88%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: Whole antisera
• Preservative: No Preservative
• Purity: Unpurified

Alternate Names for FANCG Antibody - Azide and BSA Free

• DNA repair protein XRCC9
• FAG
• FANCG
• Fanconi anemia group G protein
• Fanconi anemia, complementation group G
• Protein FACG
• X-ray repair, complementing defective, in Chinese hamster, 9
• XRCC9
• XRCC9X-ray repair complementing defective repair in Chinese hamster cells 9

Background

FANCG, involved in Fanconi anemia, confers resistance to both hygromycin and mitomycin C. FANCG contains a 5-prime GC-rich untranslated region characteristic of housekeeping genes. The putative 622-amino acid protein has a leucine-zipper motif at its N-terminus. Fanconi anemia is an autosomal recessive disorder with diverse clinical symptoms, including developmental anomalies, bone marrow failure, and early occurrence of malignancies. A minimum of 8 FA genes have been identified.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for FANCG Antibody (NB100-2566)(6)

Publications using NB100-2566

• MY Cai, CE Dunn, W Chen, BS Kochupurak, H Nguyen, LA Moreau, GI Shapiro, K Parmar, D Kozono, AD D'Andrea Cooperation of the ATM and Fanconi Anemia/BRCA Pathways in Double-Strand Break End Resection Cell Rep, 2020-02-18;30(7):2402-2415.e5. 2020-02-18 [PMID: 32075772]
• Jin L, Chun J, Pan C et al. MAST1 Drives Cisplatin Resistance in Human Cancers by Rewiring cRaf-Independent MEk Activation. Cancer Cell. 2018-07-09 [PMID: 30033091]
• Li N, Ding L, Li B et al. Functional Analysis of Fanconi Anemia Mutations in China. Exp. Hematol. 2018-07-18 [PMID: 30031030] (WB, Human)
• Huang Y, Leung JW, Lowery M et al. Modularized functions of the Fanconi anemia core complex. Cell Rep 2014-06-26 [PMID: 24910428]
• Wang Y, Leung JW, Jiang Y et al. FANCM and FAAP24 Maintain Genome Stability via Cooperative as Well as Unique Functions. Mol Cell 2013-01-16 [PMID: 23333308] (WB, Human)
• Park, S et al. Oxidative stress/damage induces multimerization and interaction of Fanconi anemia proteins. J Biol Chem;279(29):30053-9. 2004-07-16 [PMID: 15138265]

Bioinformatics

• Gene Symbol: FANCG
• Entrez:
  • 2189(Human)
• Uniprot:
  • O15287(Human)