FANCD2 Antibody (1290D)

Knockout Validated: FANCD2 Antibody (1290D) [NBP2-54808] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and FANCD2 knockout (KO) HeLa cell line. PVDF membrane was probed with 0.1 ug/mL of Rabbit Anti-Human FANCD2 Monoclonal Antibody (Catalog # NBP2-54808) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for FANCD2 at approximately 150 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.

Simple Western: FANCD2 Antibody (1290D) [NBP2-54808] - Detection of Human FANCD2 by Simple Western in HeLa and K562 cell lysate.

Immunohistochemistry: FANCD2 Antibody (1290D) [NBP2-54808] - FANCD2 was detected in immersion fixed paraffin-embedded sections of human tonsil using Rabbit Anti-Human FANCD2 Monoclonal Antibody (Catalog # NBP2-54808) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC003). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei.

Flow (Intracellular): FANCD2 Antibody (1290D) [NBP2-54808] - An intracellular stain was performed on HeLa Cells with FANCD2 (1290D) antibody NBP2-54808 (blue) and a matched isotype control MAB1050 (orange). Cells were fixed with 4% paraformaldehyde, following fixation, cells were permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by rabbit IgG APC-conjugated secondary antibody (F0111, R&D Systems).

Western Blot: FANCD2 Antibody (1290D) [NBP2-54808] - Total protein from HeLa and K562 cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 0.1 ug/ml anti-FANCD2 (catalog number NBP2-54808) in blocking buffer and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.

• Reactivity: Hu
• Applications: WB, Simple Western, Flow, Flow-IC, IHC, IHC-P, KO
• Clone: 1290D
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1.0 mg/ml

FANCD2 Antibody (1290D) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Human FANCD2 fusion protein (N-terminal fragment). [Swiss-Prot #Q9BXW9]
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: FANCD2
• Purity: Protein A or G purified from cell culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 0.1 - 0.5 ug/ml
  • Simple Western 1 ug/ml
  • Flow Cytometry 1 ug/ml
  • Immunohistochemistry 5-10 ug/ml
  • Immunohistochemistry-Paraffin 5-10 ug/ml
  • Flow (Intracellular)
  • Knockout Validated
• Control:

  FANCD2 Knockout HeLa Cell Lysate
  FANCD2 Knockout HeLa Cell Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein A or G purified from cell culture supernatant

Alternate Names for FANCD2 Antibody (1290D)

• DKFZp762A223
• FA4
• FACD
• FACDFLJ23826
• FAD
• FAD2
• FA-D2
• FADFAD2
• FANCD
• FANCD2
• Fanconi anemia group D2 protein
• Fanconi anemia, complementation group D2
• Protein FACD2

Background

Fanconi Anemia (FA) is an autosomal-recessive cancer-prone disorder characterized by congenital defects, progressive bone marrow failure, increased chromosomal breakage, defective DNA repair and cellular hypersensitivity to mitomycin C. Fanconi Anemia Complementation Group D (FANCD) is comprised of two separate proteins, FANCD1 and FANCD2. MTDH, an RNA binding protein, regulates expression of FANCD2 and FANCD1 (1). FANCD2 is mapped to chromosome 3p25.3, has a theoretical molecular weight of 166 kDa and is localized in the nucleus. FANCD2 is involved in the regulation of DNA-binding transcription factor activity and DNA stability through accurate and efficient pairing of homologs during meiosis to promote repair of double-strand DNA breaks (2). Ubiquitination is required for FANCD2 to bind to chromatin. In response to DNA damage, FANCD2 is monoubiquinated to result in colocalization with other proteins (BRCA1 AND BRCA2) involved in homology-directed DNA repair in the nucleus and is deubiquitinated upon DNA repair completion.

References

1. Bi, J., Areecheewakul, S., Li, Y., Yang, S., Zhang, Y., Ebeid, K.,... Meng, X. (2019). MTDH/AEG-1 downregulation using pristimerin-loaded nanoparticles inhibits Fanconi anemia proteins and increases sensitivity to platinum-based chemotherapy. Gynecol Oncol, 155(2), 349-358. doi:10.1016/j.ygyno.2019.08.014

2. Balcerek, J., Jiang, J., Li, Y., Jiang, Q., Holdreith, N., Singh, B.,... Tong, W. (2018). Lnk/Sh2b3 deficiency restores hematopoietic stem cell function and genome integrity in Fancd2 deficient Fanconi anemia. Nat Commun, 9(1), 3915. doi:10.1038/s41467-018-06380-1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Review for FANCD2 Antibody (NBP2-54808) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Anonymous IF Human 09/08/2017

Summary

• Application: Immunofluorescence
• Sample Tested: Human
• Species: Human
• Lot: N/A

Bioinformatics

• Gene Symbol: FANCD2