FAIM3 Antibody (992338) [Unconjugated]

Western blot shows lysates of K562 human chronic myelogenous leukemia cell line, A20 mouse B cell lymphoma cell line, and Nb2-11 rat lymphoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human/Mouse/Rat Fc mu R/FAIM3 Monoclonal Antibody (Catalog # MAB9494) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Fc mu R/FAIM3 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

HEK293 human embryonic kidney cell line transfected with (A) human FAIM3 or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Human/Mouse/Rat FAIM3 Monoclonal Antibody (Catalog # MAB9494) followed by APC-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB0041). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu, Mu, Rt
• Applications: WB, Flow, CyTOF-ready
• Clone: 992338
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

FAIM3 Antibody (992338) [Unconjugated] Summary

• Immunogen: Human embryonic kidney cell line HEK293-derived recombinant human Fc mu R/FAIM3
  Arg18-Gly251
  Accession # O60667
• Specificity: Detects human Fc mu R/FAIM3 in direct ELISAs. Detects human, mouse, and rat Fc mu R/FAIM3 in Western blots.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for FAIM3 Antibody (992338) [Unconjugated]

• FAIM3
• Fas apoptotic inhibitory molecule 3
• Fc mu R
• Fc mu receptor
• FCMR
• immunoglobulin mu Fc receptor
• Regulator of Fas-induced apoptosis Toso
• Toso
• TOSOIgM Fc receptor

Background

The human FAIM3 gene (also known as FCMR or TOSO), is a transmembrane sialoglycoprotein expressed mainly by lymphocytes. FAIM3 is a type I membrane protein with an intracellular C-terminal domain and an extracellular N-terminal domain (1). The extracellular domain has homology to the immunoglobulin variable region domains (1) and FAIM3 is identified as an Fc receptor for IgM (2, 3). The amino acid sequence of human FAIM3 is 58% and 55% identical to that of mouse and rat FAIM3, respectively. FAIM3 was shown to be over-expressed in chronic lymphocytic leukemia (CLL) (4) and associated with disease progression (5, 6). FAIM3 has also been linked to the homeostasis and activation of the innate immune system (7). Interestingly, there is growing evidence that neurodegenerative diseases are associated with the activation of the immune surveillance system. This system is responsible for controlling danger signals and responding accordingly to the magnitude and duration of the threat (8, 9).

1. Song, Y. et al (2005) J. Biol. Chem. 280(10):9618.
2. Shima H et al. (2010) Int. Immunol. 22:149.
3. Kubagawa H et al. (2009) J. Exp. Med. 206:2779.
4. Proto-Siqueira R et al. (2008) Blood, 112:394.
5. Pallasch, C et al. (2008) Blood, 112:4213.
6. Pallasch, C P et al (2009) Leukemia & lymphoma, 50:498.
7. Sigruener, A et al (2007) Biochemical and biophysical research communications, 359:723.
8. Richards R I et al (2016) Front. Neurosci. 10:193.
9. Planells-Ferrer L et al (2016) J Neurochem. 139:11.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • O60667()