DR6/TNFRSF21 Antibody

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA(Cap)
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

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Catalog# & Conjugate Size Price
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Catalog# & Formulation Size Price

DR6/TNFRSF21 Antibody Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human DR6
Gln42-Leu350
Accession # O75509
Specificity
Detects human DR6 in ELISAs and Western blots. In sandwich ELISAs, less than 0.2% cross-reactivity with recombinant human (rh) OPG and rhTNF RII is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
TNFRSF21
Purity
Immunogen affinity purified
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1 ug/mL
  • ELISA Capture (Matched Antibody Pair) 0.2-0.8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
Application Notes
ELISA Capture: Human DR6/TNFRSF21 Antibody (Catalog # AF144)
ELISA Detection: Human DR6/TNFRSF21 Biotinylated Antibody (Catalog # BAF144)
Standard: Recombinant Human DR6/TNFRSF21 Fc Chimera (Catalog # 144-DR)
Publications
Read Publications using
AF144 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for DR6/TNFRSF21 Antibody

  • BM-018
  • CD358 antigen
  • CD358
  • Death receptor 6
  • DR6
  • DR6TNFR-related death receptor 6
  • MGC31965
  • TNFRSF21
  • tumor necrosis factor receptor superfamily member 21
  • tumor necrosis factor receptor superfamily, member 21

Background

Death Receptor 6 (DR6), also known as TNFRSF21 and CD358, is a type I transmembrane protein in the TNF receptor superfamily (1). Human DR6 consists of a 308 amino acid (aa) extracellular domain (ECD) with four cysteine‑rich motifs, a 21 aa transmembrane segment, and a 285 aa palmitylated cytoplasmic region that contains one death domain (2, 3). Within the ECD, human and mouse DR6 share 82% aa sequence identity. DR6 is expressed as an approximately 110 kDa molecule that carries extensive N‑linked and O‑linked glycosylation in its extracellular region (3, 4). Among hematopoietic cells, DR6 is expressed on monocytes, resting CD4+ T cells, and pro‑, pre‑, and naïve B cells (5 ‑ 7). DR6 knockout mice exhibit a Th2‑biased immune response characterized by exaggerated Th2 and B cell responsiveness in combination with reduced Th1 cell responsiveness and inflammatory leukocyte infiltration (6 ‑ 9). DR6 knockout mice are resistant to induced airway inflammation and experimental autoimmune encephalitis but more susceptible to severe graft versus host disease (9 ‑ 11). DR6 is also expressed on developing neurons where it can bind a shed 35 kDa N‑terminal fragment of APP or a fragment of APLP2 (12, 13). This APP fragment is generated following deprivation of neurotrophic factors, and its binding to DR6 triggers DR6‑mediated axonal pruning (12). DR6 is constitutively expressed on some prostate cancer cells and can be induced by TNF‑ alpha on others (3, 4).

  1. Benschop, R. et al. (2009) Adv. Exp. Med. Biol. 647:186.
  2. Pan, G. et al. (1998) FEBS Lett. 431:351.
  3. Klima, M. et al. (2009) Biochim. Biophys. Acta 1793:1579.
  4. Kasof, G.M. et al. (2001) Oncogene 20:7965.
  5. Matesanz-Isabel, J. et al. (2011) Immunol. Lett. 134:104.
  6. Schmidt, C.S. et al. (2003) J. Exp. Med. 197:51.
  7. Liu, J. et al. (2001) Immunity 15:23.
  8. Zhao, H. et al. (2001) J. Exp. Med. 194:1441.
  9. Venkataraman, C. et al. (2006) Immunol. Lett. 106:42.
  10. Schmidt, C.S. et al. (2005) J. Immunol. 175:2286.
  11. Liu, J. et al. (2002) J. Immunol. 169:3993.
  12. Nikolaev, A. et al. (2009) Nature 457:981.
  13. Kuester, M. et al. (2011) J. Mol. Biol. 409:189.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for DR6/TNFRSF21 Antibody (AF144)(2)

We have publications tested in 2 confirmed species: Human, Rat.

We have publications tested in 2 applications: Flow Cytometry, Western Blot.


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Flow Cytometry
(1)
Western Blot
(1)
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(1)
Rat
(1)
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Product General Protocols

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Video Protocols

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Biotin BAF144

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Bioinformatics

Gene Symbol TNFRSF21
Uniprot