FADS1 Antibody

Western Blot: FADS1 Antibody [NBP2-16407] - Sample (30 ug of whole cell lysate) A: A549 7. 5% SDS PAGE gel, diluted at 1:1000.

Immunocytochemistry/Immunofluorescence: FADS1 Antibody [NBP2-16407] - Hep G2 cells were fixed in 2% paraformaldehyde/culture medium at 37oC for 30 min.Green: FADS1 protein stained by FADS1 antibody diluted at 1:500.Blue: Hoechst 33342 staining. Scale bar = 10 um.

Immunohistochemistry-Paraffin: FADS1 Antibody [NBP2-16407] - Immunohistochemical analysis of paraffin-embedded Colon ca, using antibody at 1:250 dilution.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC, IHC-P
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

FADS1 Antibody Summary

• Immunogen: Recombinant protein encompassing a sequence within the C-terminus region of human FADS1. The exact sequence is proprietary.
• Localization: Endoplasmic reticulum membrane; Multi-pass membrane protein
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: FADS1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 52 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (88%), Porcine (88%), Rat (87%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Immunogen affinity purified

Alternate Names for FADS1 Antibody

• D5D
• D5DFLJ90273
• Delta(5) desaturase
• Delta(5) fatty acid desaturase
• Delta-5 desaturase
• delta-5 fatty acid desaturase
• EC 1.14.19
• EC 1.14.19.-
• FADS1
• FADS6
• FADSD5
• FADSD5LLCDL1
• fatty acid desaturase 1
• FLJ38956
• linoleoyl-CoA desaturase (delta-6-desaturase)-like 1
• TU12

Background

The protein encoded by this gene is a member of the fatty acid desaturase (FADS) gene family. Desaturase enzymes regulate unsaturation of fatty acids through the introduction of double bonds between defined carbons of the fatty acyl chain. FADS family members are considered fusion products composed of an N-terminal cytochrome b5-like domain and a C-terminal multiple membrane-spanning desaturase portion, both of which are characterized by conserved histidine motifs. This gene is clustered with family members FADS1 and FADS2 at 11q12-q13.1; this cluster is thought to have arisen evolutionarily from gene duplication based on its similar exon/intron organization. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: FADS1
• Uniprot:
  • O60427(Human)