FABP4/A-FABP Antibody

Immunocytochemistry/Immunofluorescence: FABP4/A-FABP Antibody [NBP1-33616] - Analysis of paraformaldehyde-fixed HeLa, using FABP4 (NBP1-33616) antibody at 1:200 dilution.

Immunohistochemistry-Paraffin: FABP4/A-FABP Antibody [NBP1-33616] - FABP4 antibody detects FABP4 protein at cytoplasm and nucleus by immunohistochemical analysis. Sample: Paraffin-embedded mouse brown adipocyte. FABP4 stained by FABP4 antibody diluted at 1:500.

Western Blot: FABP4/A-FABP Antibody [NBP1-33616] - Unstimulated and stimulated 3T3-L1 whole cell extracts (20 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with FABP4 antibody diluted at 1:500. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. The differentiation stimulated medium is composed by basal medium, 10% FBS, 50 ug/ml gentamicin, 1 nM L-glutamin, 500 uM IBMX, 1 uM dexamethasone, 2 uM rosiglitazone and 1 ug/ml insulin.

Immunocytochemistry/Immunofluorescence: FABP4/A-FABP Antibody [NBP1-33616] - Paraformaldehyde-fixed mouse ESC D3, using FABP4 (adipocyte) antibody at 1:200 dilution

Western Blot: FABP4/A-FABP Antibody [NBP1-33616] -Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with FABP4 antibody [N3C3] diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301 was used to detect the primary antibody.

Western Blot: FABP4/A-FABP Antibody [NBP1-33616] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with FABP4 antibody [N3C3] diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

• Reactivity: Hu, Mu, RM
• Applications: WB, ELISA, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

FABP4/A-FABP Antibody Summary

• Description: Novus Biologicals Rabbit FABP4/A-FABP Antibody (NBP1-33616) is a polyclonal antibody validated for use in IHC, WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human FABP4/A-FABP. The exact sequence is proprietary.
• Localization: Cytoplasm, Nucleus
• Marker: Adipocyte Differentiation Marker
• Predicted Species: Rhesus Macaque (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: FABP4
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • ELISA Assay dependent
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry Assay dependent
  • Immunohistochemistry-Paraffin Assay dependent
  • Western Blot 1:500-1:3000
• Theoretical MW: 15 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  Human Adipose Whole Tissue Lysate (Adult Whole Normal)

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 1% BSA, 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for FABP4/A-FABP Antibody

• Adipocyte lipid-binding protein
• Adipocyte-type fatty acid-binding protein
• AFABP
• A-FABP
• A-FABPAFABP
• ALBP
• aP2
• FABP4
• fatty acid binding protein 4, adipocyte
• Fatty acid-binding protein 4
• fatty acid-binding protein, adipocyte

Background

Fatty acid binding protein 4 encodes the fatty acid binding protein found in adipocytes. Fatty acid binding proteins are a family of small, highly conserved, cytoplasmic proteins that bind long-chain fatty acids and other hydrophobic ligands. It is thought that FABPs roles include fatty acid uptake, transport, and metabolism.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: FABP4