FABP4/A-FABP: Novus Biologicals

AF1443

FABP4/A-FABP Antibody [Unconjugated]

(2 Reviews)

100 μg

AF1443

HAF019). A specific band was detected for FABP4/A-FABP at approximately 14 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox">

Western blot shows lysates of mouse thymus tissue and mouse heart tissue. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse/Rat FABP4/A-FABP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1443) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # <a class=

HAF019). A specific band was detected for FABP4/A-FABP at approximately 14 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of mouse thymus tissue and mouse heart tissue. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse/Rat FABP4/A-FABP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1443) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for FABP4/A-FABP at approximately 14 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_lightbox">

FABP4 was detected in immersion fixed mouse mesenchymal stem cell-derived adipocytes using 10 µg/mL Goat Anti-Mouse/Rat FABP4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1443) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # <a class=

NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_thumb" />

NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_lightbox">

FABP4 was detected in immersion fixed rat mesenchymal stem cells differentiated to adipocytes using Goat Anti-Mouse/Rat FABP4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1443) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (yellow; Catalog # <a class=

NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_thumb" />

CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the plasma membrane of epithelial cells in intestinal glands. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." class="big_lightbox">

FABP4 was detected in perfusion fixed frozen sections of mouse intestine using Goat Anti-Mouse/Rat FABP4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1443) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # <a class=

CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the plasma membrane of epithelial cells in intestinal glands. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." class="big_thumb" />

HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system." class="big_lightbox">

Simple Western lane view shows lysates of mouse thymus tissue and mouse heart tissue, loaded at 0.2 mg/mL. A specific band was detected for FABP4/A-FABP at approximately 17 kDa (as indicated) using 2.5 µg/mL of Goat Anti-Mouse/Rat FABP4/A-FABP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1443) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # <a class=

HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system." class="big_thumb" />

<b>Biological Strategies Validation. </b>A-FABP mediates expression of Dio2 via inhibition of LXR alpha .(a,b) BAT isolated from WT and A-FABP KO mice (a) fed with STC or HFD for 24 weeks or (b) subjected to room temperature (23 °C) or cold exposure (6 °C) for 24 h as indicated in Fig. 2 were subjected to immunoblotting using an antibody against A-FABP, type II iodothyronine deiodinase (D2), liver X receptor alpha (LXR alpha ) and beta -tubulin. The bar charts below are the band intensity of each protein relative to beta -tubulin and expressed as arbitrary units, N.D., not detected (n=6). (c,d) The mRNA abundance of A-FABP, Dio2 and LXR alpha in BAT of above WT and A-FABP KO mice (c) fed with STC or HFD for 24 weeks or (d) subjected to cold exposure (6 °C) for 24 h (n=6). (e) The mRNA abundance of A-FABP, LXR alpha and Dio2 in WT or A-FABP-deficient primary brown adipocytes incubated with PBS or recombinant A-FABP (rA-FABP, 2 μg ml−1) for 24 h (n=6). (f) The mRNA abundance of LXR alpha downstream target genes (SCD-1, SREBP-1c) and Dio2 in WT and A-FABP-deficient primary brown adipocytes treated with or without LXR alpha agonist TO901317 (TO;1 μM) and/or rA-FABP (2 μg ml−1) for 24 h (n=6). Uncropped western blot images are shown in Supplementary Fig. 13. Data are represented as mean±s.e.m. *P<0.05, **P<0.01 (One-way analysis of variance with Bonferroni correction for multiple comparisons). Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/28128199), licensed under a CC-BY license. Not internally tested by R&D Systems.

<b>Biological Strategies Validation. </b>A-FABP accelerates proteasomal degradation of LXR alpha .(a) Primary adipocytes derived from male 6-week-old A-FABP KO mice or WT littermates were treated with actinomycin D (actD, 1 μg ml−1) or vehicle (PBS). The mRNA level of LXR alpha was determined by real-time PCR at time points as indicated (n=4). (b,c) Primary brown adipocytes derived from male 6-week-old A-FABP KO mice or WT littermates were treated with (b) cycloheximide (CHX, 50 μg ml−1) or (c) CHX (50 μg ml−1) together with MG132 (10 μM) for different periods were subjected to immunoblotting using an antibody against LXR alpha , A-FABP and GADPH as indicated (n=4). (d) Primary brown adipocytes derived male 6-week-old A-FABP KO mice or WT littermates were infected with adenovirus overexpressing A-FABP (Ad-AFABP) or luciferase (Ad-Luci) for 48 h, followed by treatment with CHX (50 μg ml−1) for 0,6,12 and 24 h, and then subjected to immunoblotting using an antibody against LXR alpha , A-FABP and GADPH as indicated (n=4). The right panel is the band intensity of LXR alpha normalized with GAPDH, and expressed as percentage relative to baseline (0 h). Uncropped western blot images are shown in Supplementary Fig. 14. Data are represented as mean±s.e.m. *P<0.05, **P<0.01 (Students' t-test). Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/28128199), licensed under a CC-BY license. Not internally tested by R&D Systems.

$<b>Biological Strategies Validation. </b>NPGPx is a suppressor of adipogenesis via ROS regulationRelative NPGPx expression assayed by qRT-PCR (left panel) and Western blots (right panel) in the stromal vascular fraction (SVF) and adipocyte fraction isolated from the gonadal fat of C57BL/6 mice (n = 3 per group).Relative NPGPx expression in preadipocytes (Lin−CD34+CD29+Sca1+), endothelial cells (CD31+), macrophages (CD11b+) and adipocytes of adipose tissue measured by in C57BL/6 mice (n = 3 per group).NPGPx, CCAAT/enhancer-binding protein beta (C/EBP beta ) and peroxisome proliferator-activated receptor gamma (PPAR gamma ) mRNA level during induced 3T3-L1 adipogenesis (n = 5 per group).NPGPx expression assayed by Western blots during 3T3L1 adipogenesis.NBT reduction staining of wild-type SVF cells (WT), NPGPx-knockout SVF cells (KO) and NPGPx-knockout SVF cells expressing human NPGPx (KO + hNPGPx) (left panels). Quantification of NBT reduction stains 8 days after induction (n = 3 per group) (right panels). *p = 0.0002 for WT vs. KO by independent Student's t-test.Oil Red O stain of WT, KO and KO + hNPGPx SVF (left panels). Quantification of Oil Red O stain 8 days after induction (n = 3 per group) (right panels). *p = 0.0002 for WT vs. KO by independent Student's t-test.Western blots showing ap2 and adiponectin expression in WT, KO and KO + hNPGPx SVF cells 8 days after induction.NBT reduction stain of NPGPx knockdown (KD) and control (V) 3T3-L1 preadipocytes after adipogenic induction (left panels). Quantification of NBT reduction stain at day 8 (n = 3 per group) (right panels). *p = 0.0004 by independent Student's t-test.Oil Red O staining of KD and V 3T3-L1 preadipocytes after adipogenic induction (left panels). Quantification of Oil Red O stain at day 8 (n = 3 per group) (right panels). *p = 0.0005 by independent Student's t-test.Western blots showing ap2 and adiponectin expression in NPGPx KD and V 3T3-L1 preadipocytes after adipogenic stimulation.NBT reduction stain and Oil Red O stain of NPGPx KD and V 3T3-L1 cells 8 d$

<b>Biological Strategies Validation. </b>Chemotherapy leads to fat loss in diet induced obese miceA. Weight of epididymal white adipose tissue (eWAT) from LFD, HFD, CY200 and MTX50 treated mice 1 week after last chemotherapy treatment. Each treatment group consisted of 6 mice, showing mean ± SD, data representative of 2 biological replicates. Comparisons by Student's t-test: ns - not significant, * p<0.02, ** p<0.004, *** p<0.002, **** p<0.0007 B. Representative illustration of abdominal cavities measured in (A), showing that large visceral fat deposits (arrows) were reduced post chemotherapy in HFD mice. C. Relative percent frequency of eWAT-associated adipocyte stem-like cells (ASC) in HFD mice treated with MTX12.5 or CY100. Each treatment group consisted of 3 mice, showing mean ± SD, data representative of 2 biological replicates. Statistics as in (A). D. Representative confocal imagery of sorted ASC cultured in adipogenic medium displaying lipid droplets. DAPI (blue)- DNA nuclear stain, FABP4 (green)- lipid transport protein in adipocytes, LipidTOX (red)- neutral lipid stain. Image collected and cropped by CiteAb from the following open publication (//www.oncotarget.com/lookup/doi/10.18632/oncotarget.14576), licensed under a CC-BY license. Not internally tested by R&D Systems.

In vitro differentiation capacity of young and old BM-MSCs: (A) Representative micrographs from each experimental group showing morphological change after 21 days of differentiation. (B) Results of adipogenic, osteogenic and chondrogenic differentiation. Representative micrographs showing anti-FABP4, Oil Red O, anti-osteocalcin, Alizarin Red-S and anti-aggrecan staining in cultured BM-MSCs from each experimental group. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/21992089), licensed under a CC-BY license. Not internally tested by R&D Systems.

Pancreatic TRM cell recruitment during T1D progression is FABP4‐dependent. G) Representative images of immunofluorescent co‐staining of FABP4, CD69,&insulin in the pancreas of 10‐week‐old NOD mice (Scale bar 20 µm). Image collected & cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/38884133), licensed under a CC-BY license. Not internally tested by R&D Systems.

NPGPx is a suppressor of adipogenesis via ROS regulation. Western blots showing ap2 and adiponectin expression in NPGPx KD and V 3T3-L1 preadipocytes after adipogenic stimulation. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/23828861), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reactivity:

Mu, Rt

Species Glossary

Reactivity: Mu, Rt

Applications:

, , ,

Applications: , , ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	AF1443U-100UG
Alexa Fluor 405	AF1443V-100UG
Alexa Fluor 488	AF1443G-100UG
Alexa Fluor 532	AF1443X-100UG
Alexa Fluor 594	AF1443T-100UG
Alexa Fluor 647	AF1443R-100UG
Alexa Fluor 700	AF1443N-100UG
Alexa Fluor 750	AF1443S-100UG
Alexa Fluor Plus 405	AF1443AFP405-100UG
Alexa Fluor Plus 488	AF1443AFP488-100UG
Alexa Fluor Plus 555	AF1443AFP555-100UG
Alexa Fluor Plus 594	AF1443AFP594-100UG
Alexa Fluor Plus 647	AF1443AFP647-100UG
Alexa Fluor Plus 680	AF1443AFP680-100UG
Allophycocyanin	AF1443A
Biotin	BAF1443
CoraFluor 1	AF1443CL1
DyLight 350	AF1443D
DyLight 405	AF1443E
DyLight 488	AF1443K
DyLight 550	AF1443L
DyLight 594	AF1443M
DyLight 650	AF1443W
DyLight 680	AF1443Y
DyLight 755	AF1443Z
FITC	AF1443F
HRP	AF1443H
Janelia Fluor 525	AF1443JF525
Janelia Fluor 549	AF1443I
Janelia Fluor 646	AF1443J
Janelia Fluor 669	AF1443JF669
PE	AF1443P
PerCP	AF1443C
mFluor Violet 450 SE	AF1443MFV450
mFluor Violet 500 SE	AF1443MFV500
mFluor Violet 610 SE	AF1443MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF1443-SP

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AF3150

FABP4/A-FABP Antibody [Unconjugated]

(1 Review)

(22 Publications)

100 μg

AF3150

HAF019). A specific band was detected for FABP4/A-FABP at approximately 14 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox">

Western blot shows lysates of human heart tissue, rat heart tissue, mouse heart tissue, human adipose tissue, rat adipose tissue, and mouse adipose tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human FABP4/A-FABP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3150) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (<a class=

HAF019). A specific band was detected for FABP4/A-FABP at approximately 14 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of human heart tissue, rat heart tissue, mouse heart tissue, human adipose tissue, rat adipose tissue, and mouse adipose tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human FABP4/A-FABP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3150) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF019). A specific band was detected for FABP4/A-FABP at approximately 14 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox">

FABP4/A-FABP was detected in immersion fixed paraffin-embedded sections of human bladder cancer tissue using Goat Anti-Human FABP4/A-FABP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3150) at 3 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; <a class=

CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />

HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system." class="big_lightbox">

Simple Western lane view shows lysates of human heart tissue and mouse heart tissue, loaded at 0.2 mg/mL. A specific band was detected for FABP4/A-FABP at approximately 19 kDa (as indicated) using 10 µg/mL of Goat Anti-Human FABP4/A-FABP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3150) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # <a class=

HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system." class="big_thumb" />

MAB3150) coated on a Clear Polystyrene Microplate (DY990). Goat Anti-Human FABP4/A‑FABP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3150) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (DY998) followed by Substrate Solution (DY999) and stopping the enzymatic reaction with Stop Solution (DY994)." class="big_lightbox">

Recombinant Human/Mouse FABP4/A‑FABP protein was serially diluted 2-fold and captured by Rat Anti-Human/Mouse FABP4/A‑FABP Monoclonal Antibody (<a class=

MAB3150) coated on a Clear Polystyrene Microplate (DY990). Goat Anti-Human FABP4/A‑FABP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3150) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (DY998) followed by Substrate Solution (DY999) and stopping the enzymatic reaction with Stop Solution (DY994)." class="big_thumb" />

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, , ,

Applications: , , ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	AF3150U-100UG
Alexa Fluor 405	AF3150V-100UG
Alexa Fluor 488	AF3150G-100UG
Alexa Fluor 532	AF3150X-100UG
Alexa Fluor 594	AF3150T-100UG
Alexa Fluor 647	AF3150R-100UG
Alexa Fluor 700	AF3150N-100UG
Alexa Fluor 750	AF3150S-100UG
Alexa Fluor Plus 405	AF3150AFP405-100UG
Alexa Fluor Plus 488	AF3150AFP488-100UG
Alexa Fluor Plus 555	AF3150AFP555-100UG
Alexa Fluor Plus 594	AF3150AFP594-100UG
Alexa Fluor Plus 647	AF3150AFP647-100UG
Alexa Fluor Plus 680	AF3150AFP680-100UG
Allophycocyanin	AF3150A
Biotin	AF3150B
CoraFluor 1	AF3150CL1
DyLight 350	AF3150D
DyLight 405	AF3150E
DyLight 488	AF3150K
DyLight 550	AF3150L
DyLight 594	AF3150M
DyLight 650	AF3150W
DyLight 680	AF3150Y
DyLight 755	AF3150Z
FITC	AF3150F
HRP	AF3150H
Janelia Fluor 525	AF3150JF525
Janelia Fluor 549	AF3150I
Janelia Fluor 646	AF3150J
Janelia Fluor 669	AF3150JF669
PE	AF3150P
PerCP	AF3150C
mFluor Violet 450 SE	AF3150MFV450
mFluor Violet 500 SE	AF3150MFV500
mFluor Violet 610 SE	AF3150MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF3150-SP

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DFBP40

Human FABP4 Quantikine ELISA Kit

(14 Publications)

1 Kit

DFBP40

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

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NBP1-89218

FABP4/A-FABP Antibody - BSA Free

(6 Publications)

0.1 ml

NBP1-89218

Immunohistochemistry-Paraffin: FABP4/A-FABP Antibody [NBP1-89218] - Staining of human liver shows no cytoplasmic positivity in hepatocytes as expected.

Immunohistochemistry-Paraffin: FABP4/A-FABP Antibody [NBP1-89218] - Staining of human adipose tissue shows strong cytoplasmic positivity in adipocytes.

Immunohistochemistry-Paraffin: FABP4/A-FABP Antibody [NBP1-89218] - Staining of human cerebral cortex shows no cytoplasmic positivity in neurons as expected.

Immunohistochemistry-Paraffin: FABP4/A-FABP Antibody [NBP1-89218] - Staining of human placenta shows strong cytoplasmic positivity in endothelial cells.

Simple Western: FABP4/A-FABP Antibody [NBP1-89218] - Simple Western lane view shows a specific band for FABP4 in 0.2 mg/ml of RT-4 (left) and U-251MG sp (right) lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Simple Western: FABP4/A-FABP Antibody [NBP1-89218] - Electropherogram image(s) of corresponding Simple Western lane view. FABP4/A-FABP antibody was used at 1:20 dilution on RT-4 and U-251MG sp lysates(s).

Correlation between reduced iron levels and increased ferroportin expression in injured muscles. (a) Histological difference between intact control (CTL) muscle and injured muscle by hematoxylin and eosin staining. Pictures are representative of the muscle tissue from pronator quadratus (hand) and rotator cuff (shoulder) muscles (n = 10 per group). Scale bar: 100 μm. (b) Intracellular iron concentration between intact control and injured muscles (n = 30 per group). (c) mRNA expression of ferritin, hepcidin and ferroportin for intact control and injured muscles (n = 30 per group). (d) Western blot analyses for myosin heavy chain (MYH), fatty acid binding protein 4 (FABP4), ferritin, ferroportin and alpha -tubulin protein expression in intact control and injured muscles (n = 6 per group). A densitometric analysis of the Western blots is shown in the graph on the right. (e) Immunofluorescence (IF) microscopy images showing ferroportin expression. The blue signals indicate cell nuclei. Images are representative of independent tissue samples (n = 10 per group). Scale bar: 100 μm. The negative control (secondary antibody only) for the IF showed no specific signal (data not shown). Ferroportin expression levels (green staining) were assessed with densitometry using Image J software (Version 1.8.0, National Institutes of Health, Bethesda, MD, USA), shown on the right. Data represent mean +/- SEM. * p < 0.05. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/36139428), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, ,

Applications: , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Validated by:

Validated by:

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP1-89218-25ul

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NBP2-66828

FABP4/A-FABP Antibody (JM10-99)

100 ul

NBP2-66828

Immunohistochemistry-Paraffin: FABP4/A-FABP Antibody (JM10-99) [NBP2-66828] - Analysis of paraffin-embedded mouse lung tissue using anti-FABP4 antibody. Counter stained with hematoxylin.

Immunocytochemistry/Immunofluorescence: FABP4/A-FABP Antibody (JM10-99) [NBP2-66828] - Staining FABP4 in A549 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: FABP4/A-FABP Antibody (JM10-99) [NBP2-66828] - Staining FABP4 in MCF-7 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: FABP4/A-FABP Antibody (JM10-99) [NBP2-66828] - Analysis of paraffin-embedded human breast tissue using anti-FABP4 antibody. Counter stained with hematoxylin.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, ,

Applications: , ,

Host:

Rabbit Monoclonal

Host: Rabbit Monoclonal

Conjugate:

Unconjugated

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MAB3150

FABP4/A-FABP Antibody (804914) [Unconjugated]

(3 Publications)

100 μg

MAB3150

HAF005). A specific band was detected for FABP4/A-FABP at approximately 14 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox">

Western blot shows lysates of human adipose tissue and mouse adipose tissue. PVDF membrane was probed with 1 µg/mL of Rat Anti-Human FABP4/A-FABP Monoclonal Antibody (Catalog # MAB3150) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # <a class=

HAF005). A specific band was detected for FABP4/A-FABP at approximately 14 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of human adipose tissue and mouse adipose tissue. PVDF membrane was probed with 1 µg/mL of Rat Anti-Human FABP4/A-FABP Monoclonal Antibody (Catalog # MAB3150) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for FABP4/A-FABP at approximately 14 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

DY990). Goat Anti-Human FABP4/A‑FABP Antigen Affinity-purified Polyclonal Antibody (AF3150) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (DY998) followed by Substrate Solution (DY999) and stopping the enzymatic reaction with Stop Solution (DY994)." class="big_lightbox">

Recombinant Human/Mouse FABP4/A‑FABP protein was serially diluted 2-fold and captured by Rat Anti-Human/Mouse FABP4/A‑FABP Monoclonal Antibody (Catalog # MAB3150) coated on a Clear Polystyrene Microplate (<a class=

DY990). Goat Anti-Human FABP4/A‑FABP Antigen Affinity-purified Polyclonal Antibody (AF3150) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (DY998) followed by Substrate Solution (DY999) and stopping the enzymatic reaction with Stop Solution (DY994)." class="big_thumb" />

Reactivity:

Hu, Mu

Species Glossary

Reactivity: Hu, Mu

Applications:

,

Applications: ,

Host:

Rat Monoclonal

Host: Rat Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB3150U-100UG
Alexa Fluor 405	FAB3150V-100UG
Alexa Fluor 488	FAB3150G-100UG
Alexa Fluor 532	FAB3150X-100UG
Alexa Fluor 594	FAB3150T-100UG
Alexa Fluor 647	FAB3150R-100UG
Alexa Fluor 700	FAB3150N-100UG
Alexa Fluor 750	FAB3150S-100UG
Alexa Fluor Plus 405	FAB3150AFP405-100UG
Alexa Fluor Plus 488	FAB3150AFP488-100UG
Alexa Fluor Plus 555	FAB3150AFP555-100UG
Alexa Fluor Plus 594	FAB3150AFP594-100UG
Alexa Fluor Plus 647	FAB3150AFP647-100UG
Alexa Fluor Plus 680	FAB3150AFP680-100UG
Allophycocyanin	FAB3150A
Biotin	FAB3150B
CoraFluor 1	FAB3150CL1
DyLight 350	FAB3150D
DyLight 405	FAB3150E
DyLight 488	FAB3150K
DyLight 550	FAB3150L
DyLight 594	FAB3150M
DyLight 650	FAB3150W
DyLight 680	FAB3150Y
DyLight 755	FAB3150Z
FITC	FAB3150F
HRP	FAB3150H
Janelia Fluor 525	FAB3150JF525
Janelia Fluor 549	FAB3150I
Janelia Fluor 646	FAB3150J
Janelia Fluor 669	FAB3150JF669
PE	FAB3150P
PerCP	FAB3150C
mFluor Violet 450 SE	FAB3150MFV450
mFluor Violet 500 SE	FAB3150MFV500
mFluor Violet 610 SE	FAB3150MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB3150-SP

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DY3150-05

Human FABP4/A-FABP DuoSet ELISA, 5 Plate

(5 Publications)

1 Kit (for 5 Plates)

DY3150-05

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

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NBP1-33616

FABP4/A-FABP Antibody

100 ul

NBP1-33616

Western Blot: FABP4/A-FABP Antibody [NBP1-33616] - Unstimulated and stimulated 3T3-L1 whole cell extracts (20 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with FABP4 antibody diluted at 1:500. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. The differentiation stimulated medium is composed by basal medium, 10% FBS, 50 ug/ml gentamicin, 1 nM L-glutamin, 500 uM IBMX, 1 uM dexamethasone, 2 uM rosiglitazone and 1 ug/ml insulin.

Immunocytochemistry/Immunofluorescence: FABP4/A-FABP Antibody [NBP1-33616] - Paraformaldehyde-fixed mouse ESC D3, using FABP4 (adipocyte) antibody at 1:200 dilution

Western Blot: FABP4/A-FABP Antibody [NBP1-33616] -Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with FABP4 antibody [N3C3] diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301 was used to detect the primary antibody.

Reactivity:

Hu, Mu, RM

Species Glossary

Reactivity: Hu, Mu, RM

Applications:

, , ,

Applications: , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

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NBP2-82410

Mouse FABP4/A-FABP ELISA Kit (Colorimetric)

(1 Publication)

1 Kit

NBP2-82410

Reactivity:

Mu

Species Glossary

Reactivity: Mu

Applications:

Applications:

Standard Curve:

0.78 - 50 ng/mL

Sensitivity:

0.47 ng/mL

Sample Type:

Serum, plasma, cell supernatant, and other biological fluids

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NBC1-18492

Recombinant Human FABP4/A-FABP Protein

0.1 mg

NBC1-18492

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

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NBP2-90054

FABP4/A-FABP Antibody (001) - Azide and BSA Free

100 ul

NBP2-90054

Immunohistochemistry-Paraffin: FABP4/A-FABP Antibody (001) [NBP2-90054] - Staining of human FABP4 in human lung cancer with rabbit monoclonal antibody (1:2000).

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

,

Applications: ,

Host:

Rabbit Monoclonal

Host: Rabbit Monoclonal

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-90054AF350
Alexa Fluor 405	NBP2-90054AF405
Alexa Fluor 488	NBP2-90054AF488
Alexa Fluor 532	NBP2-90054AF532
Alexa Fluor 594	NBP2-90054AF594
Alexa Fluor 647	NBP2-90054AF647
Alexa Fluor 700	NBP2-90054AF700
Alexa Fluor 750	NBP2-90054AF750
Alexa Fluor Plus 405	NBP2-90054AFP405
Alexa Fluor Plus 488	NBP2-90054AFP488
Alexa Fluor Plus 555	NBP2-90054AFP555
Alexa Fluor Plus 594	NBP2-90054AFP594
Alexa Fluor Plus 647	NBP2-90054AFP647
Alexa Fluor Plus 680	NBP2-90054AFP680
Allophycocyanin	NBP2-90054APC
Biotin	NBP2-90054B
CoraFluor 1	NBP2-90054CL1
DyLight 350	NBP2-90054UV
DyLight 405	NBP2-90054V
DyLight 488	NBP2-90054G
DyLight 550	NBP2-90054R
DyLight 594	NBP2-90054DL594
DyLight 650	NBP2-90054C
DyLight 680	NBP2-90054FR
DyLight 755	NBP2-90054IR
FITC	NBP2-90054F
HRP	NBP2-90054H
Janelia Fluor 525	NBP2-90054JF525
Janelia Fluor 549	NBP2-90054JF549
Janelia Fluor 646	NBP2-90054JF646
Janelia Fluor 669	NBP2-90054JF669
PE	NBP2-90054PE
PerCP	NBP2-90054PCP
mFluor Violet 450 SE	NBP2-90054MFV450
mFluor Violet 500 SE	NBP2-90054MFV500
mFluor Violet 610 SE	NBP2-90054MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NBP2-90054-50ul

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NBP3-13879

FABP4/A-FABP Antibody (FABP4/4422)

100 μg

NBP3-13879

Protein Array: FABP4/A-FABP Antibody (FABP4/4422) [NBP3-13879] - Analysis of Protein Array containing more than 19,000 full-length human proteins using FABP4/A-FABP antibody (FABP4/4422).

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

,

Applications: ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP3-14210AF350
Alexa Fluor 405	NBP3-14210AF405
Alexa Fluor 488	NBP3-14210AF488
Alexa Fluor 532	NBP3-14210AF532
Alexa Fluor 594	NBP3-14210AF594
Alexa Fluor 647	NBP3-14210AF647
Alexa Fluor 700	NBP3-14210AF700
Alexa Fluor 750	NBP3-14210AF750
Alexa Fluor Plus 405	NBP3-14210AFP405
Alexa Fluor Plus 488	NBP3-14210AFP488
Alexa Fluor Plus 555	NBP3-14210AFP555
Alexa Fluor Plus 594	NBP3-14210AFP594
Alexa Fluor Plus 647	NBP3-14210AFP647
Alexa Fluor Plus 680	NBP3-14210AFP680
Allophycocyanin	NBP3-14210APC
Biotin	NBP3-14210B
CoraFluor 1	NBP3-14210CL1
DyLight 350	NBP3-14210UV
DyLight 405	NBP3-14210V
DyLight 488	NBP3-14210G
DyLight 550	NBP3-14210R
DyLight 594	NBP3-14210DL594
DyLight 650	NBP3-14210C
DyLight 680	NBP3-14210FR
DyLight 755	NBP3-14210IR
FITC	NBP3-14210F
HRP	NBP3-14210H
Janelia Fluor 525	NBP3-14210JF525
Janelia Fluor 549	NBP3-14210JF549
Janelia Fluor 646	NBP3-14210JF646
Janelia Fluor 669	NBP3-14210JF669
PE	NBP3-14210PE
PerCP	NBP3-14210PCP
mFluor Violet 450 SE	NBP3-14210MFV450
mFluor Violet 500 SE	NBP3-14210MFV500
mFluor Violet 610 SE	NBP3-14210MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NBP3-14210

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Category

Protein/Target

Applications

Reactivity

Citation

Antibody Validation

Reviews

Host

Available Conjugates

Format

Modification

Clonality

Clone

Images Type

Isotype

Preservative

Protein Or Peptide

Research Areas

Has Sample Size

Promotional Item

Species Glossary

FABP4 Products By Gene Name

FABP4/A-FABP Antibody [Unconjugated]

FABP4/A-FABP Antibody [Unconjugated]

Human FABP4 Quantikine ELISA Kit

FABP4/A-FABP Antibody - BSA Free

FABP4/A-FABP Antibody (JM10-99)

FABP4/A-FABP Antibody (804914) [Unconjugated]

Human FABP4/A-FABP DuoSet ELISA, 5 Plate

FABP4/A-FABP Antibody

Mouse FABP4/A-FABP ELISA Kit (Colorimetric)

Recombinant Human FABP4/A-FABP Protein

FABP4/A-FABP Antibody (001) - Azide and BSA Free

FABP4/A-FABP Antibody (FABP4/4422)