FABP4/A-FABP Antibody [Unconjugated]

Images

 
Western blot shows lysates of human heart tissue, rat heart tissue, mouse heart tissue, human adipose tissue, rat adipose tissue, and mouse adipose tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human ...read more
FABP4/A‑FABP was detected in immersion fixed paraffin-embedded sections of human bladder cancer tissue using Goat Anti-Human FABP4/A‑FABP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3150) at 3 µg/mL ...read more
Simple Western lane view shows lysates of human heart tissue and mouse heart tissue, loaded at 0.2 mg/mL. A specific band was detected for FABP4/A‑FABP at approximately 19 kDa (as indicated) using 10 µg/mL of Goat ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Formulation Size Price

FABP4/A-FABP Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human FABP4/A‑FABP
Cys2-Ala132
Accession # P15090
Specificity
Detects human FABP4/A‑FABP in direct ELISAs and Western blots. In direct ELISAs, approximately 60% cross-reactivity with recombinant mouse (rm) FABP4 is observed, approximately 30% cross‑reactivity with recombinant human (rh) FABP3 is observed, and less than 5% cross‑reactivity with rhFABP1, -2, -5, -6, -7, -8, -9, and rmFABP9 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
FABP4
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
  • Simple Western 10 ug/mL
  • Immunohistochemistry 5-15 ug/mL
Reviewed Applications
Read 1 Review rated 4
using
AF3150 in the following applications:

Publications
Read Publications using
AF3150 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for FABP4/A-FABP Antibody [Unconjugated]

  • Adipocyte lipid-binding protein
  • Adipocyte-type fatty acid-binding protein
  • AFABP
  • A-FABP
  • A-FABPAFABP
  • ALBP
  • aP2
  • FABP4
  • fatty acid binding protein 4, adipocyte
  • Fatty acid-binding protein 4
  • fatty acid-binding protein, adipocyte

Background

FABP4, also known as adipocyte P2 and A-FABP (adipocyte FABP), is a FABP family member that is expressed in adipocytes and monocyte-derived foam cells. It is a lipid transport protein that binds long chain fatty acid and retinoic acid. Human and mouse FABP4 share 91% amino acid sequence homology.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for FABP4/A-FABP Antibody (AF3150)(7)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 3 applications: ICC, IHC, Western Blot.


Filter By Application
ICC
(3)
IHC
(2)
Western Blot
(2)
All Applications
Filter By Species
Human
(6)
Mouse
(1)
All Species
Showing Publications 1 - 7 of 7.
Publications using AF3150 Applications Species
W Hu, C Jiang, D Guan, P Dierickx, R Zhang, A Moscati, GN Nadkarni, DJ Steger, RJF Loos, C Hu, W Jia, RE Soccio, MA Lazar Patient Adipose Stem Cell-Derived Adipocytes Reveal Genetic Variation that Predicts Antidiabetic Drug Response Cell Stem Cell, 2019;24(2):299-308.e6. 2019 [PMID: 30639037] (Western Blot, Human) Western Blot Human
EWY Tung, S Ahmed, V Peshdary, E Atlas Firemaster� 550 and its components isopropylated triphenyl phosphate and triphenyl phosphate enhance adipogenesis and transcriptional activity of peroxisome proliferator activated receptor (Ppar?) on the adipocyte protein 2 (aP2) promoter PLoS ONE, 2017;12(4):e0175855. 2017 [PMID: 28437481] (Western Blot, Mouse) Western Blot Mouse
K Tatebayash, Y Tanaka, A Nakano-Doi, R Sakuma, S Kamachi, M Shirakawa, K Uchida, H Kageyama, T Takagi, S Yoshimura, T Matsuyama, T Nakagomi Identification of multipotent stem cells in human brain tissue following stroke Stem Cells Dev, 2017;0(0):. 2017 [PMID: 28323540] (ICC, Human) ICC Human
Transcriptome sequencing wide functional analysis of human mesenchymal stem cells in response to TLR4 ligand Sci Rep, 2016;6(0):30311. 2016 [PMID: 27444640] (IHC, Human) IHC Human
Xia H, Bodempudi V, Benyumov A, Hergert P, Tank D, Herrera J, Braziunas J, Larsson O, Parker M, Rossi D, Smith K, Peterson M, Limper A, Jessurun J, Connett J, Ingbar D, Phan S, Bitterman P, Henke C Identification of a cell-of-origin for fibroblasts comprising the fibrotic reticulum in idiopathic pulmonary fibrosis. Am J Pathol, 2014;184(5):1369-83. 2014 [PMID: 24631025] (IHC, Human) IHC Human
Reinhardt P, Glatza M, Hemmer K, Tsytsyura Y, Thiel C, Hoing S, Moritz S, Parga J, Wagner L, Bruder J, Wu G, Schmid B, Ropke A, Klingauf J, Schwamborn J, Gasser T, Scholer H, Sterneckert J Derivation and expansion using only small molecules of human neural progenitors for neurodegenerative disease modeling. PLoS ONE, 2013;8(3):e59252. 2013 [PMID: 23533608] (ICC, Human) ICC Human
Klim JR, Li L, Wrighton PJ A defined glycosaminoglycan-binding substratum for human pluripotent stem cells. Nat. Methods, 2010;7(12):989-94. 2010 [PMID: 21076418] (ICC, Human) ICC Human

Review for FABP4/A-FABP Antibody (AF3150) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using AF3150:
Filter by Applications
IF
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
  4
reviewed by:
Anonymous
IF Human 01/07/2015
View

Summary

ApplicationImmunofluorescence
Sample TestedSee PMID 23423575
SpeciesHuman

Blocking

Blocking DetailsSee PMID 23423575

Details

Detection NotesSee PMID 23423575

Comments

Commentsalso worked well als functional antibody in cell culture experimentsPublished: See PMID 23423575Independent review from antibodyresource.com

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Recent Reviews

4
5
0
4
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1
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Anonymous
01/07/2015
Application: IF
Species: Human

Bioinformatics

Gene Symbol FABP4
Uniprot