F4/80 Antibody (1123826) [Unconjugated]

DR647RT) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane. Protocol available in COMET™ Panel Builder." > DR647RT) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane. Protocol available in COMET™ Panel Builder." title="EMR-1 was detected in immersion fixed paraffin-embedded sections of mouse spleen using Rat Anti-Mouse EMR-1 Monoclonal Antibody (Catalog # MAB11811) at 3ug/mL at 37 ° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog # TA-999-DHBH). Tissue was stained using the Alexa Fluor™ 647 Goat anti-Rat IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR647RT) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane. Protocol available in COMET™ Panel Builder." />

EMR-1 was detected in immersion fixed paraffin-embedded sections of mouse spleen using Rat Anti-Mouse EMR-1 Monoclonal Antibody (Catalog # MAB11811) at 3ug/mL at 37 ° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog # TA-999-DHBH). Tissue was stained using the Alexa Fluor™ 647 Goat anti-Rat IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # <A class=

DR647RT) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane. Protocol available in COMET™ Panel Builder.​" > DR647RT) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane. Protocol available in COMET™ Panel Builder.​" title="EMR-1 was detected in immersion fixed paraffin-embedded sections of mouse testis using Rat Anti-Mouse EMR-1 Monoclonal Antibody (Catalog # MAB11811) at 3ug/mL at 37 ° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog # TA-999-DHBH). Tissue was stained using the Alexa Fluor™ 647 Goat anti-Rat IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR647RT) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane. Protocol available in COMET™ Panel Builder.​" />

EMR-1 was detected in immersion fixed paraffin-embedded sections of mouse testis using Rat Anti-Mouse EMR-1 Monoclonal Antibody (Catalog # MAB11811) at 3ug/mL at 37 ° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog # TA-999-DHBH). Tissue was stained using the Alexa Fluor™ 647 Goat anti-Rat IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # <A class=

DR647RT) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane. Protocol available in COMET™ Panel Builder.​" > DR647RT) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane. Protocol available in COMET™ Panel Builder.​" title="EMR-1 was detected in immersion fixed paraffin-embedded sections of mouse liver using Rat Anti-Mouse EMR-1 Monoclonal Antibody (Catalog # MAB11811) at 3ug/mL at 37 ° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog # TA-999-DHBH). Tissue was stained using the Alexa Fluor™ 647 Goat anti-Rat IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR647RT) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane. Protocol available in COMET™ Panel Builder.​" />

EMR-1 was detected in immersion fixed paraffin-embedded sections of mouse liver using Rat Anti-Mouse EMR-1 Monoclonal Antibody (Catalog # MAB11811) at 3ug/mL at 37 ° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog # TA-999-DHBH). Tissue was stained using the Alexa Fluor™ 647 Goat anti-Rat IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # <A class=

DR647RT) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane. Protocol available in COMET™ Panel Builder.​" > DR647RT) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane. Protocol available in COMET™ Panel Builder.​" title="EMR-1 was detected in immersion fixed paraffin-embedded sections of mouse kidney using Rat Anti-Mouse EMR-1 Monoclonal Antibody (Catalog # MAB11811) at 3ug/mL at 37 ° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog # TA-999-DHBH). Tissue was stained using the Alexa Fluor™ 647 Goat anti-Rat IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR647RT) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane. Protocol available in COMET™ Panel Builder.​" />

EMR-1 was detected in immersion fixed paraffin-embedded sections of mouse kidney using Rat Anti-Mouse EMR-1 Monoclonal Antibody (Catalog # MAB11811) at 3ug/mL at 37 ° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog # TA-999-DHBH). Tissue was stained using the Alexa Fluor™ 647 Goat anti-Rat IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # <A class=

HAF005). A specific band was detected for F4/80 at approximately 130-300 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." > HAF005). A specific band was detected for F4/80 at approximately 130-300 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." title="Western Blot shows lysates of mouse spleen and J774A.1 mouse reticulum cell sarcoma macrophage cell line. PVDF membrane was probed with 2 µg/ml of Rat Anti-Mouse F4/80 Monoclonal Antibody (Catalog # MAB11811) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (HAF005). A specific band was detected for F4/80 at approximately 130-300 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." />

Western Blot shows lysates of mouse spleen and J774A.1 mouse reticulum cell sarcoma macrophage cell line. PVDF membrane was probed with 2 µg/ml of Rat Anti-Mouse F4/80 Monoclonal Antibody (Catalog # MAB11811) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (<a class=

VCTS021). Tissue was stained using the HRP-conjugated Anti-Rat IgG Secondary Antibody (HAF005) and counterstained with hematoxylin (blue). Specific staining was localized to the membrane. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." > VCTS021). Tissue was stained using the HRP-conjugated Anti-Rat IgG Secondary Antibody (HAF005) and counterstained with hematoxylin (blue). Specific staining was localized to the membrane. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." title="F4/80 was detected in perfusion fixed paraffin-embedded sections of mouse liver using Rat Anti-Mouse F4/80 Monoclonal Antibody (Catalog # MAB11811) at 5 µg/ml overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (VCTS021). Tissue was stained using the HRP-conjugated Anti-Rat IgG Secondary Antibody (HAF005) and counterstained with hematoxylin (blue). Specific staining was localized to the membrane. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." />

F4/80 was detected in perfusion fixed paraffin-embedded sections of mouse liver using Rat Anti-Mouse F4/80 Monoclonal Antibody (Catalog # MAB11811) at 5 µg/ml overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (<a class=

VCTS021). Tissue was stained using the HRP-conjugated Anti-Rat IgG Secondary Antibody and counterstained with hematoxylin (blue). Specific staining was localized to the membrane. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." > VCTS021). Tissue was stained using the HRP-conjugated Anti-Rat IgG Secondary Antibody and counterstained with hematoxylin (blue). Specific staining was localized to the membrane. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." title="F4/80 was detected in perfusion fixed paraffin-embedded sections of mouse spleen using Rat Anti-Mouse F4/80 Monoclonal Antibody (Catalog # MAB11811) at 5 µg/ml overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (VCTS021). Tissue was stained using the HRP-conjugated Anti-Rat IgG Secondary Antibody and counterstained with hematoxylin (blue). Specific staining was localized to the membrane. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." />

F4/80 was detected in perfusion fixed paraffin-embedded sections of mouse spleen using Rat Anti-Mouse F4/80 Monoclonal Antibody (Catalog # MAB11811) at 5 µg/ml overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (<a class=

• Reactivity: Mu
• Clone: 1123826
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated

F4/80 Antibody (1123826) [Unconjugated] Summary

• Immunogen: Chinese Hamster Ovary cell line, CHO-derived mouse EMR-1
  Met1-Ser590
  Accession # Q61549
• Specificity: Detects recombinant mouse F4/80 protein in Direct ELISA.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Reconstitution Instructions: Reconstitute lyophilized material at 0.2 mg/ml in sterile PBS. For liquid material, refer to CoA for concentration.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for F4/80 Antibody (1123826) [Unconjugated]

• ADGRE1
• cell surface glycoprotein F4/80
• DD7A5-
• DD7A5-7
• EGF-like module containing, mucin-like, hormone receptor-like sequence 1
• EGF-like module receptor 1
• EGF-like module-containing mucin-like hormone receptor-like 1
• EGF-TM7
• Emr
• EMR1 hormone receptor
• Emr1
• F4/80
• Gpf480
• Ly7
• Ly71
• lymphocyte antigen 71
• Mouse EMR1
• Rat EMR1
• TM7L
• TM7LN3

Background

F4/80, also known as EMR1, is a 160 kDa extensively glycosylated protein in the EGF-TM7 family of adhesion molecules. It is primarily expressed on macrophages and dendritic cells in mouse but is restricted to eosinophils in human. F4/80 plays a role in the development of peripheral tolerance.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for F4/80 Antibody (MAB11811). (Showing 1 - 3 of 3 FAQ).

1. Are the F4/80/EMR1 antibodies validated in Simple Western?
   • No, F4/80/EMR1 antibodies are not tested in Simple Western at this moment.
2. Does F4/80/EMR1 antibodies come in lyophilized form?
   • Yes, we carry 2 F4/80 antibodies in lyophilized form: MAB5580 and NBP1-06975
3. What is the theoretical molecular weight for F4/80/EMR1 antibodies?
   • The TMW of F4/80/EMR1 antibodies is approximately 160 kDa.