Ezrin Antibody

Images

 
Western Blot: Ezrin Antibody [NBP1-86863] - Analysis in human cell line A-431.
Immunocytochemistry/ Immunofluorescence: Ezrin Antibody [NBP1-86863] - Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane.
Immunohistochemistry-Paraffin: Ezrin Antibody [NBP1-86863] - Staining in human placenta and skeletal muscle tissues using anti-EZR antibody. Corresponding EZR RNA-seq data are presented for the same tissues.
Western Blot: Ezrin Antibody [NBP1-86863] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
Genetic Strategies: Western Blot: Ezrin Antibody [NBP1-86863] - Analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2. Remaining relative intensity is presented. Loading ...read more
Immunohistochemistry-Paraffin: Ezrin Antibody [NBP1-86863] - Staining of human stomach (upper) shows strong cytoplasmic positivity in parietal cells.
Immunohistochemistry-Paraffin: Ezrin Antibody [NBP1-86863] - Staining of human placenta shows high expression.
Immunohistochemistry-Paraffin: Ezrin Antibody [NBP1-86863] - Staining of human skeletal muscle shows low expression as expected.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, KD
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
     

Genetic Strategies

   

Order Details

Ezrin Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: REEKHQKQLERQQLETEKKRRETVEREKEQMMREKEELMLRLQDYEEKTKKAERELSEQIQRALQLEEERKRAQEEAERLEADRMAALRAKEELERQAVDQIKSQEQLAAELAEYTAKIALLE
Specificity
Specificity of human, mouse, rat Ezrin antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
EZR
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.4 ug/ml
  • Immunocytochemistry/Immunofluorescence 1 - 4 ug/ml
  • Immunohistochemistry 1:1000 - 1:2500
  • Immunohistochemistry-Paraffin 1:1000 - 1:2500
  • Knockdown Validated
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Positive Control
Ezrin Lysate (NBP2-64812)
Control Peptide
Ezrin Recombinant Protein Antigen (NBP1-86863PEP)
Publications
Read Publications using
NBP1-86863 in the following applications:

  • IHC
    2 publications

Reactivity Notes

Human reactivity reported in scientific literature (PMID: 24885195).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Ezrin Antibody

  • CVIL
  • CVL
  • Cytovillin 2
  • Cytovillin
  • DKFZp762H157
  • EZR
  • Ezrin
  • FLJ26216
  • p81
  • VIL2
  • VIL2MGC1584
  • villin 2 (ezrin)
  • Villin 2
  • villin-2

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Ezrin Antibody (NBP1-86863)(2)

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Ezrin Antibody (NBP1-86863) (0)

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Additional Ezrin Products

Bioinformatics Tool for Ezrin Antibody (NBP1-86863)

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Bioinformatics

Gene Symbol EZR
Entrez