EZH1 Antibody

Images

 
Western Blot: EZH1 Antibody [NB100-56358] - WB detection of EZH1 protein in (A ) Ramos cells lysate, (B) Daudi cells lysate and (C) Peptide blocked - Daudi cells lysate by using EZH1 antibody at a concentration of 2 ...read more
Western Blot: EZH1 Antibody [NB100-56358] - Detection of EZH1 in mouse (lane 1) and human (lane 2) spleen lysate using this antibody at 2 ug/mL.
Western Blot: EZH1 Antibody [NB100-56358] - 15 ug of HeLa nuclear extract on PVDF membrane. Image from verified customer.

Product Details

Summary
Reactivity Hu, Mu, Bv, Mk, PmSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

EZH1 Antibody Summary

Immunogen
A portion of amino acids 350-400 of human EZH1 was used as the immunogen.
Localization
Nucleus
Predicted Species
Monkey (100%), Primate (100%), Bovine (93%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
EZH1
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 2 - 4 ug/mL
Application Notes
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
85.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
Human Spleen Whole Tissue Lysate (Adult Whole Normal)
Mouse Spleen Whole Tissue Lysate (Adult Whole Normal)
EZH1 Overexpression Lysate
Reviewed Applications
Read 1 Review rated 3
using
NB100-56358 in the following application:

Publications
Read Publications using
NB100-56358 in the following applications:

  • WB
    2 publications

Reactivity Notes

Immunogen sequence similarity with rat and dog (86%).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for EZH1 Antibody

  • EC 2.1.1.43
  • enhancer of zeste homolog 1 (Drosophila)
  • Enhancer of zeste homolog 1
  • ENX-2
  • histone-lysine N-methyltransferase EZH1
  • KIAA0388enhancer of zeste (Drosophila) homolog 1
  • KMT6B

Background

EZH1 is a human homolog of the Drosophila gene Enhancer of zeste E(z), a member of the polycomb group of transcriptional repressors. In mice, EZH1 is a regulator of homeotic gene expression implicated in the assembly of repressive protein complexes in chromatin. The strong sequence conservation between species suggests potential role for EZH1 in human development as a transcriptional regulator and as a component of protein complexes that stably maintain heterochromatin.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: WB
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Publications for EZH1 Antibody (NB100-56358)(2)

Review for EZH1 Antibody (NB100-56358) (1) 31

Average Rating: 3
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NB100-56358:
Filter by Applications
WB
(1)
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Filter by Species
Human
(1)
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Images Ratings Applications Species Date Details
Western Blot EZH1 NB100-56358
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reviewed by:
Anonymous
WB Human 01/31/2014
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Summary

ApplicationWestern Blot
Sample TestedHeLa nuclear extract on PVDF membrane
SpeciesHuman

Blocking

Blocking Details5% NFM, 1 hr, RT

Primary Anitbody

Dilution Ratio1:500 in 2.5% NFM Overnight at 4 degrees

Secondary Antibody

Secondary Descriptionanti-rabbit, HRP
Secondary Concentration1:10,000

Details

Detection NotesShort exposure of ~5 seconds on Bio-Rad imager. Signal blew out quickly and background was high after 30 sec exposure.

Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for EZH1 Antibody (NB100-56358) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

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Bioinformatics Tool for EZH1 Antibody (NB100-56358)

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Research Areas for EZH1 Antibody (NB100-56358)

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Blogs on EZH1.

EZH1 has more to offer than gene repression
EZH1 is part of the Polycomb-group family of proteins, which are responsible for remodeling chromatin in genes and modulating epigenetic silencing during development.  Specifically, EZHI is a component of PRC2, or polycomb repressive complex-2.  PR...  Read full blog post.

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Recent Reviews

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5
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1
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1
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Anonymous
01/31/2014
Application: WB
Species: Human

Bioinformatics

Gene Symbol EZH1
Entrez
Uniprot
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