EED Antibody


Western blot shows lysates of Jurkat human acute T cell leukemia cell line and HT‑29 human colon adenocarcinoma cell line, human colon cancer tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human EED more

Product Details

Reactivity Hu, MuSpecies Glossary
Applications WB

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EED Antibody Summary

E. coli-derived recombinant human EED
Accession # O75530
Detects human and mouse EED in direct ELISAs and Western blots.
Immunogen affinity purified
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Western Blot 1 ug/mL
Read Publications using
AF5827 in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
No Preservative
Immunogen affinity purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for EED Antibody

  • EED
  • embryonic ectoderm development
  • HEED
  • polycomb protein EED
  • WAIT1
  • WAIT1
  • WAIT-1
  • WD protein associating with integrin cytoplasmic tails 1


EED (Embryonic ectoderm development; also WAIT-1) is a 51 kDa member of the WD repeat ESC family of proteins. It is widely expressed, and constitutes part of both the PRC1 and PRC2 nuclear complexes. PRC1 contains EED, Suz12, RbAp46 and Ezh1 and is found in nonproliferating tissue. PRC2 differs only by the presence of Ezh2 and is found in proliferating tissues. Both complexes methylate histone H3 and act as gene repressors. Human EED is 441 amino acids in length. It contains three phosphorylation sites (Ser34; Thr55; Thr57) followed by seven WD40 domains (aa 91‑441). There are two alternate start sites, one at Met15, and another that is 94 aa upstream of the standard site, begins with a Val and generates a 69 kDa isoform. A third isoform variant shows an insertion of 25 aa after Ser323, while a fourth variant shows a premature truncation after Lys400. Human and mouse EED are identical in amino acid sequence.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for EED Antibody (AF5827)(5)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 3 applications: IHC-P, Simple Western, Western Blot.

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Simple Western
Western Blot
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Showing Publications 1 - 5 of 5.
Publications using AF5827 Applications Species
VanDyke, D;Xu, L;Sargunas, PR;Gilbreth, RN;Baca, M;Gao, C;Hunt, J;Spangler, JB; Redirecting the specificity of tripartite motif containing-21 scaffolds using a novel discovery and design approach The Journal of biological chemistry 2023-10-20 [PMID: 37866632] (Western Blot, Human) Western Blot Human
Gabrielle J. Dardis, Jun Wang, Jeremy M. Simon, Gang Greg Wang, Albert S. Baldwin An EZH2-NF-kappa B regulatory axis drives expression of pro-oncogenic gene signatures in triple negative breast cancer iScience 2023-07-21 [PMID: 37416481]
Frances Potjewyd, Anne-Marie W. Turner, Joshua Beri, Justin M. Rectenwald, Jacqueline L. Norris-Drouin, Stephanie H. Cholensky et al. Degradation of Polycomb Repressive Complex 2 with an EED-Targeted Bivalent Chemical Degrader Cell Chemical Biology 2020-01-01 [PMID: 31831267] (Simple Western) Simple Western
EM Michalak, MJG Milevskiy, RM Joyce, JF Dekkers, PR Jamieson, B Pal, CA Dawson, Y Hu, SH Orkin, WS Alexander, GJ Lindeman, GK Smyth, JE Visvader Canonical PRC2 function is essential for mammary gland development and affects chromatin compaction in mammary organoids PLoS Biol., 2018-08-06;16(8):e2004986. 2018-08-06 [PMID: 30080881] (IHC-P, Mouse) IHC-P Mouse
DJ Micati, GR Hime, EA McLaughlin, HE Abud, KL Loveland Differential expression profiles of conserved Snail transcription factors in the mouse testis Andrology, 2018-01-30;0(0):. 2018-01-30 [PMID: 29381885] (IHC-P, Mouse) IHC-P Mouse

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Product General Protocols

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Secondary Antibodies


Isotype Controls

Additional EED Products

Blogs on EED.

Microglia: pruning shears for homeostatic maintenance in the brain
By Jennifer Sokolowski, MD, PhD.Microglia play a critical role in pruning neurons and synapses during homeostatic maintenance in the adult brain.1 A recent study by Ayata et al. (2018) identified regional differe...  Read full blog post.

EZH1 has more to offer than gene repression
EZH1 is part of the Polycomb-group family of proteins, which are responsible for remodeling chromatin in genes and modulating epigenetic silencing during development.  Specifically, EZHI is a component of PRC2, or polycomb repressive complex-2.  PR...  Read full blog post.

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Gene Symbol EED