Western blot shows lysates of Jurkat human acute T cell leukemia cell line and HT‑29 human colon adenocarcinoma cell line, human colon cancer tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human EED ...read more
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Immunogen affinity purified
Reconstitute at 0.2 mg/mL in sterile PBS.
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for EED Antibody
embryonic ectoderm development
polycomb protein EED
WD protein associating with integrin cytoplasmic tails 1
EED (Embryonic ectoderm development; also WAIT-1) is a 51 kDa member of the WD repeat ESC family of proteins. It is widely expressed, and constitutes part of both the PRC1 and PRC2 nuclear complexes. PRC1 contains EED, Suz12, RbAp46 and Ezh1 and is found in nonproliferating tissue. PRC2 differs only by the presence of Ezh2 and is found in proliferating tissues. Both complexes methylate histone H3 and act as gene repressors. Human EED is 441 amino acids in length. It contains three phosphorylation sites (Ser34; Thr55; Thr57) followed by seven WD40 domains (aa 91‑441). There are two alternate start sites, one at Met15, and another that is 94 aa upstream of the standard site, begins with a Val and generates a 69 kDa isoform. A third isoform variant shows an insertion of 25 aa after Ser323, while a fourth variant shows a premature truncation after Lys400. Human and mouse EED are identical in amino acid sequence.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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