EWSR1 Antibody

Images

 
Western Blot: EWSR1 Antibody [NBP1-28798] - (0.1ug/ml) staining of nuclear HeLa lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: EWSR1 Antibody [NBP1-28798] - (3.8ug/ml) staining of Human Small Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IHC-P, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

EWSR1 Antibody Summary

Immunogen
Peptide with sequence C-TSYDQSSYSQQNTYG corresponding to internal region according to NP_005234.1, NP_001156757.1, NP_001156758.1, NP_001156759.1.
Epitope
internal region
Specificity
This antibody is expected to recognize all five reported isoforms (NP_053733.2; NP_005234.1; NP_001156757.1; NP_001156758.1; NP_001156759.1).
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
EWSR1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.3 - 1 ug/ml
  • Immunohistochemistry 4 - 8 ug/ml
  • Immunohistochemistry-Paraffin 1:10 - 1:500
  • Peptide ELISA Detection limit 1:64000
Application Notes
WB: Approx. 90 kDa band observed in HeLa nuclear lysates (calculated MW of 68.5 kDa band according to NP_053733.1). The observed molecular weight corresponds to earlier findings with different antibodies from other sources. IHC-P: Human small intestine shows nuclear staining in entrocytes. Data aobtained from a previous batch (different goat).
Publications
Read Publication using NBP1-28798.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Mouse, Rat, Porcine, Bovine.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for EWSR1 Antibody

  • bK984G1.4
  • Ewing sarcoma breakpoint region 1 protein
  • Ewing sarcoma breakpoint region 1
  • Ewings sarcoma EWS-Fli1 (type 1) oncogene
  • EWS oncogene
  • EWSRNA-binding protein EWS

Background

This gene encodes a multifunctional protein that is involved in various cellular processes, including gene expression, cell signaling, and RNA processing and transport. The protein includes an N-terminal transcriptional activation domain and a C-terminal RNA-binding domain. Chromosomal translocations between this gene and various genes encoding transcription factors result in the production of chimeric proteins that are involved in tumorigenesis. These chimeric proteins usually consist of the N-terminal transcriptional activation domain of this protein fused to the C-terminal DNA-binding domain of the transcription factor protein. Mutations in this gene, specifically a t(11;22)(q24;q12) translocation, are known to cause Ewing sarcoma as well as neuroectodermal and various other tumors. Alternative splicing of this gene results in multiple transcript variants. Related pseudogenes have been identified on chromosomes 1 and 14. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for EWSR1 Antibody (NBP1-28798)(1)


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Bioinformatics

Gene Symbol EWSR1
Entrez
Uniprot