EWSR1 Antibody

Western Blot: EWSR1 Antibody [NBP1-28798] - (0.3ug/ml) staining of nuclear HeLa lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Immunohistochemistry-Paraffin: EWSR1 Antibody [NBP1-28798] - Negative Control showing staining of paraffin embedded Human Kidney, with no primary antibody.

Immunohistochemistry-Paraffin: EWSR1 Antibody [NBP1-28798] - (7ug/ml) staining of paraffin embedded Human Kidney. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.

• Reactivity: Hu, Mu, Rt, Po, Bv
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

EWSR1 Antibody Summary

• Immunogen: Peptide with sequence C-TSYDQSSYSQQNTYG corresponding to internal region according to NP_005234.1, NP_001156757.1, NP_001156758.1, NP_001156759.1.
• Epitope: internal region
• Specificity: This antibody is expected to recognize all five reported isoforms (NP_053733.2; NP_005234.1; NP_001156757.1; NP_001156758.1; NP_001156759.1).
• Predicted Species: Mouse (100%), Rat (100%), Porcine (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: EWSR1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-7 ug/ml
  • Immunohistochemistry-Paraffin 5-7 ug/ml
  • Peptide ELISA Detection limit 1:64000
  • Western Blot 0.3-1 ug/ml
• Application Notes: Western blot: Approx. 90kDa band observed in nuclear lysates of cell line HeLa (calculated MW of 68.5kDa according to NP_053733.1). This molecular weight is routinely observed by other sources.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for EWSR1 Antibody

• bK984G1.4
• Ewing sarcoma breakpoint region 1 protein
• Ewing sarcoma breakpoint region 1
• Ewings sarcoma EWS-Fli1 (type 1) oncogene
• EWS oncogene
• EWS
• EWSR1
• EWSRNA-binding protein EWS

Background

This gene encodes a multifunctional protein that is involved in various cellular processes, including gene expression, cell signaling, and RNA processing and transport. The protein includes an N-terminal transcriptional activation domain and a C-terminal RNA-binding domain. Chromosomal translocations between this gene and various genes encoding transcription factors result in the production of chimeric proteins that are involved in tumorigenesis. These chimeric proteins usually consist of the N-terminal transcriptional activation domain of this protein fused to the C-terminal DNA-binding domain of the transcription factor protein. Mutations in this gene, specifically a t(11;22)(q24;q12) translocation, are known to cause Ewing sarcoma as well as neuroectodermal and various other tumors. Alternative splicing of this gene results in multiple transcript variants. Related pseudogenes have been identified on chromosomes 1 and 14. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for EWSR1 Antibody (NBP1-28798)(1)

Publication using NBP1-28798

• Toretsky JA, Erkizan V, Levenson A et al. Oncoprotein EWS-FLI1 activity is enhanced by RNA helicase A. Cancer Res 2006-06-01 [PMID: 16740692]

Bioinformatics

• Gene Symbol: EWSR1
• Entrez:
  • 2130(Human)
  • 14030(Mouse)
  • 289752(Rat)
• Uniprot:
  • NP_053733.1(Human)