EVI-1 Overexpression Lysate (Native)

Images

 
Western Blot: EVI1 Overexpression Lysate (Adult Normal) [NBL1-10364] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for EVI1.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

EVI-1 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC221770 and based on accession number NM_005241. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens ecotropic viral integration site 1 (EVI1), transcript variant 2, mRNA.
Gene
MECOM

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
118.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for EVI-1

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for EVI-1 Overexpression Lysate (Native)

  • AML1-EVI-1 fusion protein
  • AML1-EVI-1
  • ecotropic viral integration site 1
  • Ecotropic virus integration site 1 protein homolog
  • EVI1
  • EVI-1
  • EVI1MDS1-EVI1
  • MDS1 and EVI1 complex locus protein EVI1
  • MDS1 and EVI1 complex locus protein MDS1
  • MDS1 and EVI1 complex locus
  • MDS1
  • MECOM
  • MGC163392
  • MGC97004
  • Myelodysplasia syndrome 1 protein
  • myelodysplasia syndrome 1
  • Myelodysplasia syndrome-associated protein 1
  • oncogene EVI1
  • PRDM3
  • zinc finger protein Evi1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP1-89105
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
AF6295
Species: Hu, Mu
Applications: WB, IHC
NBP1-92025
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
H00027130-P02
Species: Hu
NBP1-19097
Species: Hu
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP, PLA
AF5414
Species: Hu
Applications: WB, Simple Western
NBP1-80695
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P, IF
NB600-1071
Species: Mu, Rt
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP
NBP1-06069
Species: Hu
Applications: WB, IHC, IHC-P, PEP-ELISA
H00171023-M05
Species: Hu
Applications: WB, ELISA
MAB17791
Species: Hu, Mu
Applications: WB
NBP1-87769
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-89827
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P, IF
NB100-55256
Species: Hu
Applications: WB, IHC-P, IP
NBP1-89644
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NBP1-88707
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-01294
Species: Hu, Ca, Pm
Applications: WB, Flow, ICC/IF, IHC, IHC-P
NBP2-37576
Species: Hu, Mu, Pm
Applications: WB, ELISA, Flow, ICC/IF, IHC
NBP2-02023
Species: Hu
Applications: WB, ICC/IF
NBL1-10364
Species: Hu
Applications: WB

Publications for EVI-1 Lysate (NBL1-10364) (0)

There are no publications for EVI-1 Lysate (NBL1-10364).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for EVI-1 Lysate (NBL1-10364) (0)

There are no reviews for EVI-1 Lysate (NBL1-10364). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for EVI-1 Lysate (NBL1-10364) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional EVI-1 Products

Bioinformatics Tool for EVI-1 Lysate (NBL1-10364)

Discover related pathways, diseases and genes to EVI-1 Lysate (NBL1-10364). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for EVI-1 Lysate (NBL1-10364)

Discover more about diseases related to EVI-1 Lysate (NBL1-10364).
 

Pathways for EVI-1 Lysate (NBL1-10364)

View related products by pathway.

PTMs for EVI-1 Lysate (NBL1-10364)

Learn more about PTMs related to EVI-1 Lysate (NBL1-10364).

Blogs on EVI-1

There are no specific blogs for EVI-1, but you can read our latest blog posts.

Customers Who Bought This Also Bought

EVI-1 Antibody
28100002-0.1mg

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our EVI-1 Overexpression Lysate (Native) and receive a gift card or discount.

Bioinformatics

Gene Symbol MECOM
Entrez