ETV5/ERM Antibody

Images

 
Western Blot: ETV5/ERM Antibody [NBP2-14950] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with ERM antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody ...read more
Immunocytochemistry/ Immunofluorescence: ETV5/ERM Antibody [NBP2-14950] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: ERM protein stained by ERM antibody diluted at 1:500. Red: Phalloidin, a ...read more
Immunohistochemistry-Paraffin: ETV5/ERM Antibody [NBP2-14950] - Mouse colon. ERM antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Western Blot: ETV5/ERM Antibody [NBP2-14950] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with ERM antibody diluted at 1:5000. ...read more
Immunohistochemistry-Paraffin: ETV5/ERM Antibody [NBP2-14950] - PC14 xenograft, using ERM antibody at 1:100 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, BvSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

ETV5/ERM Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human ERM. The exact sequence is proprietary.
Predicted Species
Rat (95%), Porcine (98%), Bovine (98%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ETV5
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:1000-1:10000
Application Notes
IHC-Fr Assay dependent
Theoretical MW
58 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using
NBP2-14950 in the following applications:

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Chicken (88%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7), 20% Glycerol
Preservative
0.025% Proclin 300
Purity
Immunogen affinity purified

Alternate Names for ETV5/ERM Antibody

  • ERM
  • ERMEts-related protein ERM
  • ETS translocation variant 5
  • ets variant 5
  • ets variant gene 5 (ets-related molecule)
  • ets-related molecule
  • ETV5

Background

Binds to DNA sequences containing the consensus nucleotide core sequence GGAA.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for ETV5/ERM Antibody (NBP2-14950)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: IF/IHC.


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IF/IHC
(1)
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Filter By Species
Mouse
(1)
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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol ETV5
Uniprot