Kilon/NEGR1 Antibody - BSA Free

Immunocytochemistry/Immunofluorescence: Kilon/NEGR1 Antibody [NBP1-83116] - Staining of human cell line U-2 OS shows localization to actin filaments. Antibody staining is shown in green.

Immunohistochemistry-Paraffin: Kilon/NEGR1 Antibody [NBP1-83116] - Staining of human tonsil shows no positivity in germinal center cells as expected.

Immunohistochemistry-Paraffin: Kilon/NEGR1 Antibody [NBP1-83116] - Staining of human cerebellum shows weak to moderate cytoplasmic positivity in cells in molecular layer.

Immunohistochemistry-Paraffin: Kilon/NEGR1 Antibody [NBP1-83116] - Staining of human cerebral cortex shows moderate positivity in neuropil.

Immunohistochemistry-Paraffin: Kilon/NEGR1 Antibody [NBP1-83116] - Staining of human prostate shows moderate cytoplasmic positivity in smooth muscle cells.

• Reactivity: Hu, Mu, Rt
• Applications: ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Kilon/NEGR1 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit Kilon/NEGR1 Antibody - BSA Free (NBP1-83116) is a polyclonal antibody validated for use in IHC and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: AGGDKWSVDPRVSISTLNKRDYSLQIQNVDVTDDGPYTCSVQTQHTPRTMQVHLTVQVPPKIYDISNDMTVNEGTNVTLTCLATGKPEPSISWRHISPSAKPFENGQYLDIYGITRDQAGEYECSA
• Predicted Species: Mouse (98%), Rat (98%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NEGR1
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200-1:500
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
• Control Peptide:

  Kilon/NEGR1 Protein (NBP1-83116PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for Kilon/NEGR1 Antibody - BSA Free

• a kindred of IgLON
• IgLON family member 4
• IGLON4DMML2433
• Kilon
• KILONMGC46680
• NEGR1
• neuronal growth regulator 1
• Neurotractin
• Ntra

Background

NEGR1 may be involved in cell-adhesion. May function as a trans-neural growth-promoting factor in regenerative axonsprouting in the mammalian brain

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NEGR1
• Entrez:
  • 257194(Human)
• Uniprot:
  • Q7Z3B1(Human)