NL007) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." alt="ETV5 was detected in immersion fixed BT-20 human breast cancer cell line using Mouse Anti-Human ETV5 Monoclonal Antibody (Catalog # MAB7107) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red, upper panel; Catalog # NL007) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB7107-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-17656-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-55689-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-98876-50ul | |||
![Western Blot: ETV5/ERM Antibody (7C10) [H00002119-M02] - ETV5 monoclonal antibody (M02), clone 7C10. Analysis of ETV5 expression in NIH/3T3.](http://images.novusbio.com/fullsize/ETV5-ERM-Antibody-7C10-Western-Blot-H00002119-M02-img0010.jpg "Western Blot: ETV5/ERM Antibody (7C10) [H00002119-M02] - ETV5 monoclonal antibody (M02), clone 7C10. Analysis of ETV5 expression in NIH/3T3.")
![Western Blot: ETV5/ERM Antibody (7C10) [H00002119-M02] - ETV5 monoclonal antibody (M02), clone 7C10. Analysis of ETV5 expression in PC-12.](http://images.novusbio.com/fullsize/ETV5-ERM-Antibody-7C10-Western-Blot-H00002119-M02-img0007.jpg)
![Western Blot: ETV5/ERM Antibody (7C10) [H00002119-M02] - ETV5 monoclonal antibody (M02), clone 7C10 Analysis of ETV5 expression in Hela S3 NE.](http://images.novusbio.com/fullsize/ETV5-ERM-Antibody-7C10-Western-Blot-H00002119-M02-img0008.jpg)
![Western Blot: ETV5/ERM Antibody (7C10) [H00002119-M02] - ETV5 monoclonal antibody (M02), clone 7C10. Analysis of ETV5 expression in Raw 264.7.](http://images.novusbio.com/fullsize/ETV5-ERM-Antibody-7C10-Western-Blot-H00002119-M02-img0009.jpg)
![ELISA: ETV5/ERM Antibody (7C10) [H00002119-M02] - Detection limit for recombinant GST tagged ETV5 is approximately 0.03ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/ETV5-ERM-Antibody-7C10-ELISA-H00002119-M02-img0011.jpg)
![Western Blot: ETV5/ERM Antibody (PSH02-64) [NBP3-32318] - Western blot analysis of ETV5/ERM on different lysates with Rabbit anti-ETV5/ERM antibody (NBP3-32318) at 1/1,000 dilution. Lane 1: PC-3M cell lysate Lane 2: SK-MEL-28 cell lysate Lane 3: HT-29 cell lysate Lane 4: SH-SY5Y cell lysate Lane 5: A549 cell lysate Lane 6: HEK-293 cell lysate Lane 7: Hela cell lysate Lane 8: HCT116 cell lysate Lane 9: Neuro-2a cell lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 58 kDa Observed band size: 70 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32318) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32318_rabbit-etv5-erm-mab-psh02-64-278202416285265.jpg "Western Blot: ETV5/ERM Antibody (PSH02-64) [NBP3-32318] - Western blot analysis of ETV5/ERM on different lysates with Rabbit anti-ETV5/ERM antibody (NBP3-32318) at 1/1,000 dilution. Lane 1: PC-3M cell lysate Lane 2: SK-MEL-28 cell lysate Lane 3: HT-29 cell lysate Lane 4: SH-SY5Y cell lysate Lane 5: A549 cell lysate Lane 6: HEK-293 cell lysate Lane 7: Hela cell lysate Lane 8: HCT116 cell lysate Lane 9: Neuro-2a cell lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 58 kDa Observed band size: 70 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32318) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: ETV5/ERM Antibody (PSH02-64) [NBP3-32318] - Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-ETV5/ERM antibody (NBP3-32318) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32318) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32318_rabbit-etv5-erm-mab-psh02-64-298202414262628.jpg)
![Immunocytochemistry/ Immunofluorescence: ETV5/ERM Antibody (PSH02-64) [NBP3-32318] - Immunocytochemistry analysis of SK-MEL-28 cells labeling ETV5/ERM with Rabbit anti-ETV5/ERM antibody (NBP3-32318) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-ETV5/ERM antibody (NBP3-32318) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32318_rabbit-etv5-erm-mab-psh02-64-29202410334078.jpg)
![Flow Cytometry: ETV5/ERM Antibody (PSH02-64) [NBP3-32318] - Flow cytometric analysis of SK-MEL-28 cells labeling ETV5/ERM. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32318, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32318_rabbit-etv5-erm-mab-psh02-64-29202416372177.jpg)
![Western Blot: ETV5/ERM Antibody (3B10) [H00002119-M01] - ETV5 monoclonal antibody (M01), clone 3B10 Analysis of ETV5 expression in Hela S3 NE.](http://images.novusbio.com/fullsize/ETV5-ERM-Antibody-3B10-Western-Blot-H00002119-M01-img0004.jpg "Western Blot: ETV5/ERM Antibody (3B10) [H00002119-M01] - ETV5 monoclonal antibody (M01), clone 3B10 Analysis of ETV5 expression in Hela S3 NE.")
![Sandwich ELISA: ETV5/ERM Antibody (3B10) [H00002119-M01] - Detection limit for recombinant GST tagged ETV5 is approximately 0.3ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/ETV5-ERM-Antibody-3B10-Sandwich-ELISA-H00002119-M01-img0003.jpg)
![Immunohistochemistry-Paraffin: ETV5/ERM Antibody [NBP3-17656] - Staining of human cerebral cortex shows strong nuclear positivity in neuronal cells and glial cells.](http://images.novusbio.com/fullsize/ETV5-ERM-Antibody-Immunohistochemistry-Paraffin-NBP3-17656-img0001.jpg "Immunohistochemistry-Paraffin: ETV5/ERM Antibody [NBP3-17656] - Staining of human cerebral cortex shows strong nuclear positivity in neuronal cells and glial cells.")
![Western Blot: ETV5/ERM Antibody [NBP2-55689] - Analysis in human cell line RT-4.](http://images.novusbio.com/fullsize/ETV5-ERM-Antibody-Western-Blot-NBP2-55689-img0002.jpg "Western Blot: ETV5/ERM Antibody [NBP2-55689] - Analysis in human cell line RT-4.")
![Immunocytochemistry/Immunofluorescence: ETV5/ERM Antibody [NBP2-55689] - Staining of human cell line SK-MEL-30 shows localization to nucleoplasm.](http://images.novusbio.com/fullsize/ETV5-ERM-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-55689-img0001.jpg)
![Immunohistochemistry-Paraffin: ETV5/ERM Antibody [NBP2-98876] - Immunochemical staining of human ETV5/ERM in human testis with rabbit polyclonal antibody (1:10000, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/ETV5-ERM-Antibody-Immunohistochemistry-Paraffin-NBP2-98876-img0002.jpg "Immunohistochemistry-Paraffin: ETV5/ERM Antibody [NBP2-98876] - Immunochemical staining of human ETV5/ERM in human testis with rabbit polyclonal antibody (1:10000, formalin-fixed paraffin embedded sections).")
![Immunohistochemistry-Paraffin: ETV5/ERM Antibody [NBP2-98876] - Immunochemical staining of human ETV5/ERM in human spleen with rabbit polyclonal antibody (1:10000, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/ETV5-ERM-Antibody-Immunohistochemistry-Paraffin-NBP2-98876-img0001.jpg)
![Western Blot: ERM/Etv5 Overexpression Lysate (Adult Normal) [NBL1-10361] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for ERM/Etv5.](http://images.novusbio.com/fullsize/ERM-Etv5-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-10361-img0002.jpg "Western Blot: ERM/Etv5 Overexpression Lysate (Adult Normal) [NBL1-10361] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for ERM/Etv5.")
