SH2B1 Antibody

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Western blot shows lysates of LNCaP human prostate cancer cell line, Neuro‑2A mouse neuroblastoma cell line, and Rat‑2 rat embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Goat ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

SH2B1 Antibody Summary

Immunogen
E. coli-derived recombinant human SH2B1
Pro317-Leu467
Accession # Q9NRF2
Specificity
Detects human, mouse, and rat SH2B1 in Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
SH2B1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Sterile PBS to a final concentration of 0.2 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for SH2B1 Antibody

  • FLJ30542
  • KIAA1299
  • Pro-rich, PH and SH2 domain-containing signaling mediator
  • PSM
  • SH2 domain-containing protein 1B
  • SH2 domain-containing putative adapter SH2-B
  • SH2B adapter protein 1
  • SH2B adaptor protein 1
  • SH2-B signaling protein
  • SH2B1
  • SH2BDKFZp547G1110

Background

SH2B1 (Src-Homology 2 Domain-Containing protein B1/1B; also SH2B1 alpha, PSM and SH2B adaptor protein 1) is a member of the SH2B adapter family of proteins. Although its predicted MW is 80 kDa, it runs anomalously at 90-102 kDa in SDS-Page. SH2B1 is a nucleocytoplasmic protein that is widely expressed in cells such as skeletal muscle cells, neurons, and adipocytes. It serves as a linker between Jak2 and multiple downstream molecules such as Rac and IRS1. It does this following phosphorylation on a number of potential sites. It also promotes the enzymatic activity of associated receptor kinases, thus potentiating ligand-receptor interactions. SH2B1 appears to bind to nonphosphorylated Jaks as a monomer, while Jak phosphorylation induces SH2B1 dimerization. SH2B1 is further reported to form a homopentamer, and to oligomerize with SH2B2. Human SH2B1( alpha ) is 756 amino acids amino acids (aa) in length. It contains one dimerization segment (aa 24-85), an NLS (aa 224-233), one pleckstrin homology region (aa 249-378) and an SH2 domain (aa 521-625). At least three utilized phosphorylation sites exist. There are at least two splice variants for SH2B1. The 90-92 kDa SH2B1 beta isoform shows a 39 aa substitution for aa 633-756, while the SH2B1 gamma isoform possesses a 50 aa substitution for the same aa range (i.e., aa 633-756). Over aa 317-467, human SH2B1 shares 92% aa identity with mouse SH2B1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol SH2B1
Entrez
Uniprot